Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,335,762	IS5 (–) +4 bp	intergenic (+304/‑66)	yciX → / → acnA	uncharacterized protein/aconitate hydratase 1; aconitase A

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1335762 =	0 (0.000)	17 (0.460)	17/256	1.4	100%	intergenic (+304/‑69)	yciX/acnA	uncharacterized protein/aconitate hydratase 1; aconitase A
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1335765	0 (0.000)	50 (1.350)	39/256	0.1	100%	intergenic (+307/‑66)	yciX/acnA	uncharacterized protein/aconitate hydratase 1; aconitase A
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

GCTGTAGTAGTGGTAAGTTTTATCTTTGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1335902‑1335762 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT  <  NC_000913/2290113‑2289978                                                                                                                                                                                                                                                                                       GCTGTAGTAGTGGTAAGTTTTATCTTTGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTG                                                                                                                                  <  7:91279/149‑1       GTAGTGGTAAGTTTTATCTTTGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGATTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAAC                                                                                                                             <  4:44297/148‑1                  TTTTATCTTTGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATA                                                                                                                 >  2:85298/1‑149                         TTTGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGAT                                                                                                           >  1:197309/1‑148                           TGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATC                                                                                                          <  1:85298/147‑1                                       TGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTG                                                                                            >  5:157884/1‑149                                                          TAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTC                                                                         <  6:157884/149‑1                                                                      CATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCAT                                                             <  5:148974/149‑1                                                                                 CTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATAT                                                                                                                >  4:26987/1‑87                                                                                 CTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATAT                                                                                                                <  3:26987/87‑1                                                                                                 CGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTC                                  <  2:197309/149‑1                                                                                                  GAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAT                                                                       >  7:8586/1‑111                                                                                                  GAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAT                                                                       <  8:8586/111‑1                                                                                                        CTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCAGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGT                            >  1:110044/1‑148                                                                                                                     GGGTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCAGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGAC              <  2:110044/149‑1                                                                                                                       GTGAATATTAAAGATAACACAAAGGAAGGTGCGAACAAGTCCCTGAT                                                                                                                  <  4:154962/47‑1                                                                                                                                 AAGATAACACAAAGGAAGGGGCGAACAAGTCCCGGATATGAGATCATGTTTGTCATCTGGAGCCATAGCACAGGGTTCATCATGAGACATCAACTTACCGTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT  >  7:125121/1‑149                                                                                                                                                                                                                                                                                       GCTGTAGTAGTGGTAAGTTTTATCTTTGGCCTGCAACGTGTCCTTACTGGCTTCTCGTAGGGTTGACGACATAGCTCCTCCTTAATGACAGGGTTGCGAAGCCCTGATTCTCTTCAGGGTGAATATTAAAGATAACACAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1335902‑1335762 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT  <  NC_000913/2290113‑2289978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.