New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1333679	46 (1.240)	6 (0.170)	6/244	3.6	11.9%	intergenic (+34/‑176)	topA/cysB	DNA topoisomerase I, omega subunit/N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
	?	NC_000913	= 1333691	45 (1.280)					intergenic (+46/‑164)	topA/cysB	DNA topoisomerase I, omega subunit/N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTT  >  NC_000913/1333537‑1333826                                                                                                                                               |                                                                                                                                                    cTTTAGACGTAACACCAAACAGCAATATGTCTCATCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATATAAGGTTGCTGGCAAATGGGAGGAAGGAAAAAAATAACCGTGAATTCTGGCAGGAGTATATAAACAAAgggacccg                                                                                                                                                >  6:142417/1‑143 (MQ=255)        cGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGc                                                                                                                                        <  6:170626/149‑1 (MQ=255)         gTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGcc                                                                                                                                       >  1:96984/1‑149 (MQ=255)              aCCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGGTTTTATAAACAAAGGGTCGCGAAAGCGGCCCtttt                                                                                                                                  <  7:156015/149‑1 (MQ=255)                  aaCAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTa                                                                                                                               <  4:128040/148‑1 (MQ=255)                  aaCAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTa                                                                                                                               >  3:128040/1‑148 (MQ=255)                      gcattctGTCTCTTCGGAAAACGACGGAAAGGCGACTGGCTGGTCAGCTTTTTATGTTGTTGGCATATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCTGGTTTTTATAAACAAAGGGTCGCGATAGCGGCCCTTTTTTATTGCa                                                                                                                          <  5:142417/143‑1 (MQ=255)                           atGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCg                                                                                                                                          <  8:36072/128‑1 (MQ=255)                           atGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCg                                                                                                                                          >  7:36072/1‑128 (MQ=255)                                  ttCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttc                                                                                                              >  5:58570/1‑149 (MQ=255)                                    cGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttc                                                                                                              <  2:96984/147‑1 (MQ=255)                                         aaaGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCCGGTTTTTCTAAACAAAGCGCCGCGAAAGCGGCCCTTTTTTAATGCATATTATTTTTTCCTCacac                                                                                                       <  4:21807/149‑1 (MQ=255)                                         aaaGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac                                                                                                       >  2:175961/1‑149 (MQ=255)                                         aaaGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac                                                                                                       >  8:126490/1‑149 (MQ=255)                                         aaaGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac                                                                                                       <  7:126490/149‑1 (MQ=255)                                         aaaGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac                                                                                                       >  1:180711/1‑149 (MQ=255)                                                     gCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAg                                                                                           >  2:196087/1‑149 (MQ=255)                                                       gACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGc                                                                                         >  3:135446/1‑149 (MQ=255)                                                             ctggTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATaaa                                                                                   <  1:196087/149‑1 (MQ=255)                                                                gTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATg                                                                                 <  4:58824/148‑1 (MQ=255)                                                                   aGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAtt                                                                                                                             <  7:62571/101‑1 (MQ=255)                                                                   aGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAtt                                                                                                                             >  8:62571/1‑101 (MQ=255)                                                                              ttGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAg                                                                  <  6:58570/149‑1 (MQ=255)                                                                                gATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTg                                                                         >  3:97558/1‑140 (MQ=255)                                                                                gATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTg                                                                         <  4:97558/140‑1 (MQ=255)                                                                                 aTGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAAt                                                                                  >  6:136701/1‑130 (MQ=255)                                                                                 aTGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAAt                                                                                  <  5:136701/130‑1 (MQ=255)                                                                                             ttGAAGGAAAAAAATAACCTTTAATTCTGTCATGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTtta                                                   <  3:932/149‑1 (MQ=255)                                                                                             ttGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAgg                                                                                          <  1:31821/110‑1 (MQ=255)                                                                                             ttGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAgg                                                                                          >  2:31821/1‑110 (MQ=255)                                                                                                 aGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTttattat                                               <  4:135446/149‑1 (MQ=255)                                                                                                                tttAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTa                                      >  2:143718/1‑143 (MQ=255)                                                                                                                tttAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTa                                      <  1:143718/143‑1 (MQ=255)                                                                                                                    aTTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCAcc                             <  2:180711/148‑1 (MQ=255)                                                                                                                          tCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTa                                                               >  1:148221/1‑108 (MQ=255)                                                                                                                          tCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTa                                                               <  2:148221/108‑1 (MQ=255)                                                                                                                           cAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCAAGgt                     >  4:9361/1‑149 (MQ=255)                                                                                                                            aGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATc                                         <  7:136397/128‑1 (MQ=255)                                                                                                                            aGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATc                                         >  8:136397/1‑128 (MQ=255)                                                                                                                              gTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGgtgt                   >  1:188565/1‑148 (MQ=255)                                                                                                                               tttttATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa                 >  2:98924/1‑149 (MQ=255)                                                                                                                                        aCAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTAttt                                                                                                                  >  2:65637/1‑43 (MQ=255)                                                                                                                                        aCAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTAttt                                                                                                                  <  1:65637/43‑1 (MQ=255)                                                                                                                                        aCAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAAc        >  7:69616/1‑149 (MQ=255)                                                                                                                                          aaaGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGa      <  8:69616/149‑1 (MQ=255)                                                                                                                                              ggTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGtt  <  1:98924/149‑1 (MQ=255)                                                                                                                                              ggTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGtt  <  1:175961/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    CTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTT  >  NC_000913/1333537‑1333826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.