Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,237,875 T→C 5.2% M102T (ATG→ACG)  dadA → D‑amino acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,237,8750TC 5.2% 239.8 / 2.8 76M102T (ATG→ACG) dadAD‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (36/36);  new base C (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGA  >  NC_000913/1237727‑1238021
                                                                                                                                                    |                                                                                                                                                  
gcACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACAt                                                                                                                                                    >  8:351830/1‑149 (MQ=255)
 cACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACAt                                                                                                                                                    <  1:192593/148‑1 (MQ=255)
  aCCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATg                                                                                                                                                   >  5:116106/1‑148 (MQ=255)
      tGGGCGACACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGaaa                                                                                                                                               >  3:214538/1‑148 (MQ=255)
          cggcACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACaaa                                                                                                                                          <  5:322682/149‑1 (MQ=255)
          cggcACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACaaa                                                                                                                                          <  2:96798/149‑1 (MQ=255)
          cgacACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACaaa                                                                                                                                          <  4:214538/146‑1 (MQ=255)
             cACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAggg                                                                                                                                       <  3:369122/149‑1 (MQ=255)
                 aGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGa                                                                                                                                   <  5:345041/149‑1 (MQ=255)
                   gtgtGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATg                                                                                                                                 <  6:116106/149‑1 (MQ=255)
                   gtgtGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATg                                                                                                                                 <  3:179158/149‑1 (MQ=255)
                      tgCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTg                                                                                                                              >  2:379249/1‑149 (MQ=255)
                         cTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACCCCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGt                                                                                                                           >  3:295611/1‑149 (MQ=255)
                          tttAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTc                                                                                                                          >  8:235986/1‑149 (MQ=255)
                          tttAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTc                                                                                                                          >  3:337537/1‑149 (MQ=255)
                              aaaGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGc                                                                                                                      >  8:89278/1‑149 (MQ=255)
                               aaGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCg                                                                                                                     >  4:10765/1‑149 (MQ=255)
                                  cGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGaa                                                                                                                  <  7:89278/149‑1 (MQ=255)
                                    aTTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATa                                                                                                                >  6:213403/1‑149 (MQ=255)
                                        aaTGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAAc                                                                                                                                             >  6:82238/1‑116 (MQ=255)
                                         ctGGATGTTCCAGCGCCATGCTCCGCTGGCGGTTCGTCTCGACGGTACGAAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGcc                                                                                                           <  4:262290/148‑1 (MQ=255)
                                           ggATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGt                                                                                                         >  5:396379/1‑149 (MQ=255)
                                             aggTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCGCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGa                                                                                                       <  3:129969/147‑1 (MQ=255)
                                              tGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACACGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAg                                                                                                             >  8:5093/1‑142 (MQ=255)
                                              tGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACACGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAg                                                                                                             <  7:11396/142‑1 (MQ=255)
                                              tGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACACGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAg                                                                                                             <  7:5093/142‑1 (MQ=255)
                                              tGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACACGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAg                                                                                                             >  8:11396/1‑142 (MQ=255)
                                                  ccAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGc                                                                                                   <  2:396661/148‑1 (MQ=255)
                                                   cAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGcc                                                                                                  <  4:337537/148‑1 (MQ=255)
                                                   cAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCt                                                                                                 >  5:294319/1‑149 (MQ=255)
                                                     gcgcCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGaaaa                                                                                                                                              >  8:15923/1‑102 (MQ=255)
                                                     gcgcCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGaaaa                                                                                                                                              <  7:15923/102‑1 (MQ=255)
                                                     gcgcCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTCGCAAATGTTACGTTACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACCGCCGTGATTGCCGGa                                                                                               >  1:236835/1‑149 (MQ=255)
                                                      cgcCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGaa                                                                                              <  8:388706/149‑1 (MQ=255)
                                                         cATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAg                                                                                            >  4:364910/1‑148 (MQ=255)
                                                          aTGCGCCGCTGGCGGTTCGTCTCGCCGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTCGCGGAATACAGCCGTGATAGCCTGACAGCa                                                                                          >  1:369219/1‑149 (MQ=255)
                                                             cggctcTGGCGGTTGGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTTCGTAACTGCGACACCAGCCACTACATGGAAAACAATGGGCGTATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCAtt                                                                                        <  6:294319/143‑1 (MQ=255)
                                                             cgccgcTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCAtt                                                                                        <  3:33663/148‑1 (MQ=255)
                                                             cgccgcTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTg                                                                                       <  3:60729/149‑1 (MQ=255)
                                                                       ggTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGaaa                                                                             <  3:25461/149‑1 (MQ=255)
                                                                       ggTTCGTCTCGACGGTACGCAGTTCCAGTGGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGaaa                                                                             <  1:379249/149‑1 (MQ=255)
                                                                        gTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAAc                                                                            <  7:351830/149‑1 (MQ=255)
                                                                               tcGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACCCCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATAtt                                                                     >  5:93165/1‑149 (MQ=255)
                                                                                 gACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATAtt                                                                     <  8:151179/147‑1 (MQ=255)
                                                                                 gACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCa                                                                   <  5:213403/149‑1 (MQ=255)
                                                                                 gACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCa                                                                   <  6:182560/149‑1 (MQ=255)
                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAg                                                                                                                                         >  5:10391/1‑71 (MQ=255)
                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAg                                                                                                                                         <  6:10391/71‑1 (MQ=255)
                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGTCGAAACCAATATTCAGTa                                                                <  4:227920/144‑1 (MQ=255)
                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGTCGAAACCAATATTCAGTa                                                                >  3:227920/1‑144 (MQ=255)
                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAg                                                           <  5:346975/149‑1 (MQ=255)
                                                                                          cAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACACAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAgg                                                          <  5:176051/149‑1 (MQ=255)
                                                                                             ttCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCg                                                       >  8:95337/1‑149 (MQ=255)
                                                                                                cAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGt                                                      >  3:132691/1‑147 (MQ=255)
                                                                                                 aGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAg                                                   <  7:95337/149‑1 (MQ=255)
                                                                                                  gTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGGCAgg                                                  >  4:292286/1‑149 (MQ=255)
                                                                                                     gAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTg                                               >  1:396546/1‑149 (MQ=255)
                                                                                                            aTGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCt                                        <  3:364910/149‑1 (MQ=255)
                                                                                                                ggCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCaa                                    >  8:222998/1‑149 (MQ=255)
                                                                                                                 gCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAACCCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAAc                                   >  2:304810/1‑149 (MQ=255)
                                                                                                                 gCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAAc                                   >  6:409907/1‑149 (MQ=255)
                                                                                                                      tGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGaa                                                            >  1:235375/1‑119 (MQ=255)
                                                                                                                      tGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGaa                                                            <  2:235375/119‑1 (MQ=255)
                                                                                                                              aaCTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCg                       >  7:290789/1‑148 (MQ=255)
                                                                                                                               aCTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaa                     <  8:290789/149‑1 (MQ=255)
                                                                                                                                 tGCGACACCAGCCACTACATGGAAAACAAAGGGCGGTTGGTGCGTCTGGCGGAATTCAGCCGTGATTGCGTGATAGCTTTGCGCGGCGAAACGAAGATTGTGTATGATGGGCGGCAGGGTGGGGGGGTGCGTGTGTTCCGTagggatct                   >  6:324829/1‑142 (MQ=255)
                                                                                                                                 tGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaac                    >  2:70554/1‑148 (MQ=255)
                                                                                                                                  gCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGt                                                                 >  5:382090/1‑102 (MQ=255)
                                                                                                                                  gCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGt                                                                 <  6:382090/102‑1 (MQ=255)
                                                                                                                                         cAGCCACTACATCGAAACCAAAGGGCGGATGGTGCGTCTGCCGGAATACAGCCGTGATAGCCAGACAGCATGGCGCGCCGAAACAAAAATTCAGTAAGAAGGGCTTCAGGGTGGGACGCAGCAACTGTTCCGTACCGcacacaagt              >  8:381525/1‑141 (MQ=255)
                                                                                                                                           gCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAACCCAATATTCAGTATGAAGGGCGTCAGGGTGGGCCGCTGCAACTGTTCCGTACCGAACACCAGTATGa          >  3:315906/1‑148 (MQ=255)
                                                                                                                                            ccACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGaa         >  4:272945/1‑148 (MQ=255)
                                                                                                                                              aCTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTa                                                                >  5:289724/1‑91 (MQ=255)
                                                                                                                                              aCTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTa                                                                <  6:289724/91‑1 (MQ=255)
                                                                                                                                              aCTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt      <  4:132691/149‑1 (MQ=255)
                                                                                                                                                  cATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAACGCATTGCGCGCCGCAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCgc  >  3:86482/1‑148 (MQ=255)
                                                                                                                                                  cATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGa  <  3:10765/149‑1 (MQ=255)
                                                                                                                                                  cATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCGGCAACTGTTCCGTACCGAACAACAGTATGAAAATGc    <  6:396379/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGA  >  NC_000913/1237727‑1238021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: