Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,400,366 A→G 6.0% Y470H (TAC→CAC)  abgT ← p‑aminobenzoyl‑glutamate transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,400,3660AG 6.0% 202.1 / 3.0 66Y470H (TAC→CAC) abgTp‑aminobenzoyl‑glutamate transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base G (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGTGAAAGCCAAG  >  NC_000913/1400221‑1400507
                                                                                                                                                 |                                                                                                                                             
cAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagtaa                                                                                                                                              <  4:180554/147‑1 (MQ=255)
 aaGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagtaagt                                                                                                                                            <  7:54968/148‑1 (MQ=255)
   gTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAGAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTAcc                                                                                                                                         >  5:13335/1‑149 (MQ=255)
         tcAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagtaagta                                                                                                                                           >  6:265498/1‑141 (MQ=255)
         tcAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagtaagta                                                                                                                                           <  5:265498/141‑1 (MQ=255)
         tcAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagtaa                                                                                                                                              <  8:96767/138‑1 (MQ=255)
         tcAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagtaa                                                                                                                                              >  7:96767/1‑138 (MQ=255)
           aGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTcg                                                                                                                                 >  5:325673/1‑149 (MQ=255)
                  gTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTgg                                                                                                                          <  2:252367/149‑1 (MQ=255)
                     ctctctTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGtt                                                                                                                        <  4:401790/148‑1 (MQ=255)
                      tctctTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGCATAGTAAGTACCCAGTTTCGCGTCTGGTTTg                                                                                                                      >  4:264205/1‑149 (MQ=255)
                      tctctTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTg                                                                                                                      >  8:45329/1‑149 (MQ=255)
                      tctctTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTg                                                                                                                      >  8:299575/1‑149 (MQ=255)
                          ttAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAGAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTa                                                                                                                    <  6:13335/147‑1 (MQ=255)
                          ttAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTa                                                                                                                    <  8:278605/147‑1 (MQ=255)
                          ttAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAAGAGTAAGTACCCCGTTTCGCGTCGGGTTTGTAGc                                                                                                                  >  3:320339/1‑149 (MQ=255)
                            aaGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAg                                                                                                                   <  4:310600/146‑1 (MQ=255)
                             aGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGtt                                                                                                               <  6:85758/149‑1 (MQ=255)
                             aGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGt                                                                                                                <  6:102128/148‑1 (MQ=255)
                             aGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGt                                                                                                                <  8:235712/148‑1 (MQ=255)
                               aCAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGc                                                                                                             <  5:407028/149‑1 (MQ=255)
                                cAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCa                                                                                                            <  7:45329/149‑1 (MQ=255)
                                cAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCa                                                                                                            <  4:249623/149‑1 (MQ=255)
                                   aggTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCGATACCACCAAATAGATAAGTGGATAGGGCAAGTCTAACGAATAGTAAGTAGCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGa                                                                                                         <  3:113539/147‑1 (MQ=255)
                                   aCGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGGTTCGCGTCTGGGTTGTAGCGTTGCAgg                                                                                                          >  3:250360/1‑148 (MQ=255)
                                    cGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGaa                                                                                                        <  6:325673/149‑1 (MQ=255)
                                     gTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTGAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAAt                                                                                                       >  6:164332/1‑149 (MQ=255)
                                     gTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAAt                                                                                                       >  4:136513/1‑149 (MQ=255)
                                         gTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATcc                                                                                                     >  7:387313/1‑147 (MQ=255)
                                            aaTACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGa                                                                                                 <  1:18470/148‑1 (MQ=255)
                                            aaTACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGa                                                                                                 <  7:299575/148‑1 (MQ=255)
                                                 ccGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAACAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGt                                                                                           <  6:279379/149‑1 (MQ=255)
                                                 ccGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGt                                                                                           >  1:423812/1‑149 (MQ=255)
                                                  cGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTGAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTg                                                                                          <  5:164332/149‑1 (MQ=255)
                                                      ccTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAAc                                                                                      <  7:162407/149‑1 (MQ=255)
                                                         aTCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACaa                                                                                    >  5:158041/1‑148 (MQ=255)
                                                            ggCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAgg                                                                                <  3:136513/149‑1 (MQ=255)
                                                                    gACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTACCGTTGCAGGAATCCAAGAAAAAGTGGAACAAAAGTAGATACCg                                                                        >  1:184245/1‑149 (MQ=255)
                                                                       aaGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGgcg                                                                     >  7:33595/1‑149 (MQ=255)
                                                                           tACCACGCCAGCAAGATAAGCAGCCATACCACCAAAAATATAAGTGGATAGGTCAAGACTAACTAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAATAAACa                                                                                             <  2:19972/121‑1 (MQ=255)
                                                                           tACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACa                                                                                             >  1:19972/1‑121 (MQ=255)
                                                                             ccACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGgaga                                                                             <  4:122548/135‑1 (MQ=255)
                                                                             ccACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGgaga                                                                             >  3:122548/1‑135 (MQ=255)
                                                                             ccACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAg                                                               <  8:284852/149‑1 (MQ=255)
                                                                             ccACGACAGCTACATCATCATGCATACCTCCATAAAGATAATTGTATAGGGCAAGACTAACGAAAAGTAAGTTCGTAGTTTCGCGTATGGTTTGTAGCGTTGCAGGAATCCAAGAAACATTGGAACAAAAGGAGATACCTGCGCTaaat                                                               <  6:99546/149‑2 (MQ=255)
                                                                              cACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAgg                                                              >  5:213130/1‑149 (MQ=255)
                                                                              cACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAgg                                                              >  2:395295/1‑149 (MQ=255)
                                                                               aCGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGAGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAgg                                                              >  3:231618/1‑148 (MQ=255)
                                                                                      ccacctCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGCCTACCGAAGAGTAACTACCCCGTTGCGCGGCTGGTTGGTAGCGTGGCAGGACGCCAAGCACCAGGGGACCAACAGGCGCTCCCGGCGCTAACGGCCAGACg                                                       >  4:12010/6‑148 (MQ=255)
                                                                                      cAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACgg                                                      <  8:387313/149‑1 (MQ=255)
                                                                                             agcagcCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGt                                                <  2:423812/148‑1 (MQ=255)
                                                                                                 gcCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGc                                            >  5:174984/1‑148 (MQ=255)
                                                                                                    aTACAACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTAGTAGCGTTGCAGGAATCCAAGAAACATTGGAACAAAAGGACATACAGGCGCTAAAGGCAATACGGAGTAGTAGGCAAt                                         >  2:83377/1‑148 (MQ=255)
                                                                                                     tACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATAc                                       >  8:84619/1‑149 (MQ=255)
                                                                                                        caccaAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGaa                                    <  6:213130/149‑1 (MQ=255)
                                                                                                        caccaAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGa                                     >  5:155442/1‑148 (MQ=255)
                                                                                                        caccaAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGa                                     >  7:380839/1‑148 (MQ=255)
                                                                                                         accaAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGa                                     >  5:396291/1‑147 (MQ=255)
                                                                                                           caAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAACAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGaaa                                   >  3:337164/1‑147 (MQ=255)
                                                                                                            aaaaaGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAagag                                >  3:109241/1‑149 (MQ=255)
                                                                                                            aaaaaGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAagag                                >  1:350263/1‑149 (MQ=255)
                                                                                                                   tAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTgc                         <  8:33595/149‑1 (MQ=255)
                                                                                                                    aaGTGGATAGGGCAAGACTAACGAATAGTGAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACa                                                                                             <  7:331328/80‑1 (MQ=255)
                                                                                                                    aaGTGGATAGGGCAAGACTAACGAATAGTGAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACa                                                                                             >  8:331328/1‑80 (MQ=255)
                                                                                                                            aGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGcc                >  5:264186/1‑149 (MQ=255)
                                                                                                                              ggCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCgg              <  2:30757/149‑1 (MQ=255)
                                                                                                                               gCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGAGGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCggg             <  4:71450/149‑1 (MQ=255)
                                                                                                                                cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGt            >  8:256463/1‑149 (MQ=255)
                                                                                                                                   gACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGTGaa         <  6:158041/149‑1 (MQ=255)
                                                                                                                                    aCTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGTGa          >  4:390994/1‑147 (MQ=255)
                                                                                                                                      tAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGTGAAAGc      >  4:199421/1‑149 (MQ=255)
                                                                                                                                          gAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGTGAAAGCCAAg  >  7:140318/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CAAGTAATCTCAGCATCCGTTCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCGCAAATGCCGGGTGAAAGCCAAG  >  NC_000913/1400221‑1400507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑