Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,857 C→T 5.7% intergenic (‑99/+16) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,8570CT 5.7% 337.5 / 5.9 105intergenic (‑99/+16)yecJ/azuCDUF2766 family protein/acid‑inducible small membrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (52/47);  new base T (4/2);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CAACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGC  >  NC_000913/1987710‑1988005
                                                                                                                                                    |                                                                                                                                                    
caaCCACATCAGAAACCAATTCCTGATCGGAATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                     <  4:84927/149‑1 (MQ=255)
 aaCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                     >  4:344616/1‑148 (MQ=255)
  aCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                     >  7:127125/1‑147 (MQ=255)
  aCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCg                                                                                                                                                     >  1:263058/1‑147 (MQ=255)
   ccACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAg                                                                                                                                                  >  5:383584/1‑149 (MQ=255)
      caTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAg                                                                                                                                               <  2:328597/149‑1 (MQ=255)
      caTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAg                                                                                                                                               >  7:331704/1‑149 (MQ=255)
            aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttttt                                                                                                                                         <  7:424339/149‑1 (MQ=255)
             aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                        <  1:57445/149‑1 (MQ=255)
               ccAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAACCTGCTCCGGCAGGtttttttgt                                                                                                                                     <  6:238048/149‑4 (MQ=255)
                cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                        <  2:43777/146‑1 (MQ=255)
                cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGtttttt                                                                                                                                        >  1:43777/1‑146 (MQ=255)
                cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGtt                                                                                                                                     <  8:127125/149‑1 (MQ=255)
                cAATTCCTGATCGGCATTCAGCGGCTGCGACATCGTAAACTCCTGATAGCATTTTGAAAGCAGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGt                                                                                                                                      >  1:327093/1‑148 (MQ=255)
                   ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGcc                                                                                                                                                      <  8:271454/129‑1 (MQ=255)
                     ccTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCg                                                                                                                                 <  8:143231/148‑1 (MQ=255)
                         aTCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGaa                                                                                                                              <  5:89372/147‑1 (MQ=255)
                         aTCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAAc                                                                                                                             <  7:52581/148‑1 (MQ=255)
                          tCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCTGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACa                                                                                                                            >  7:55194/1‑148 (MQ=255)
                          tCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACat                                                                                                                           >  3:237163/1‑149 (MQ=255)
                           cGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACata                                                                                                                          >  6:116861/1‑149 (MQ=255)
                                ttCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTTATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGcc                                                                                                                     >  4:76451/1‑149 (MQ=255)
                                 tCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCt                                                                                                                    >  7:96850/1‑149 (MQ=255)
                                   aGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTgg                                                                                                                  <  5:116861/149‑1 (MQ=255)
                                   aGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTgg                                                                                                                  >  7:419963/1‑149 (MQ=255)
                                       gCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGt                                                                                                              <  3:344616/149‑1 (MQ=255)
                                         tGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                            >  4:225689/1‑149 (MQ=255)
                                          gggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATAtt                                                                                                                        >  8:408153/1‑136 (MQ=255)
                                          gggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATAtt                                                                                                                        <  7:408153/136‑1 (MQ=255)
                                              cATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCtt                                                                                                       <  7:375144/149‑1 (MQ=255)
                                                   tAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                                                  <  1:140266/149‑1 (MQ=255)
                                                    aaaCTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACg                                                                                                 >  1:213335/1‑149 (MQ=255)
                                                       cTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                                                  >  3:113667/1‑145 (MQ=255)
                                                       cTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                                                  <  4:113667/145‑1 (MQ=255)
                                                        tcatGATAGCATATTGAAAGCCGTAATAGTAGCTACTTCACATATTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACATTCCGGAGCAGGTTTTTTTTTAACGGAACATATTGCCTTGCGGTACGTCTTTGAACGTCtt                                                                                             >  4:147439/4‑149 (MQ=255)
                                                        tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGTGCAGGTTTTTTGTTATCGGAACATTTTGCCTGGCGGTACGTCTTTGCACGTCtt                                                                                             >  1:255737/1‑149 (MQ=255)
                                                        tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCtt                                                                                             >  6:329122/1‑149 (MQ=255)
                                                        tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCtt                                                                                             >  3:87101/1‑149 (MQ=255)
                                                         ccTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttt                                                                                                                                           <  4:215614/102‑1 (MQ=255)
                                                         ccTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGttt                                                                                                                                           >  3:215614/1‑102 (MQ=255)
                                                          cTGATAGCATTTTGAAAGCTGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg                                                                                            <  8:55194/148‑1 (MQ=255)
                                                          cTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                                  >  7:296236/1‑110 (MQ=255)
                                                          cTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATc                                                                                                                                  <  8:296236/110‑1 (MQ=255)
                                                             aTAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCaa                                                                                         >  1:123065/1‑149 (MQ=255)
                                                              tAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa                                                                                       <  7:55589/149‑1 (MQ=255)
                                                                   ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                                  >  7:290123/1‑149 (MQ=255)
                                                                   ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                                  >  2:407821/1‑149 (MQ=255)
                                                                     ttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGGCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAtt                                                                                 <  4:326521/148‑1 (MQ=255)
                                                                       gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                                               <  5:158834/132‑1 (MQ=255)
                                                                       gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                                               >  6:158834/1‑132 (MQ=255)
                                                                        aaaGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAAc                                                                             <  2:283819/149‑1 (MQ=255)
                                                                         acgCCGTTATAGTAGCGACTTCACATCTTGAGCGTTAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACa                                                                            <  5:246940/147‑1 (MQ=255)
                                                                          aGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACa                                                                            <  2:123065/149‑1 (MQ=255)
                                                                          aGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTTAACGTCTTGCAATAGTTATTGAACAt                                                                           <  2:327093/149‑1 (MQ=255)
                                                                             cGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCt                                                                                              <  6:32616/127‑1 (MQ=255)
                                                                             cGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCt                                                                                              >  5:32616/1‑127 (MQ=255)
                                                                                 ataGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTTATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACtttt                                                                     <  3:76451/148‑1 (MQ=255)
                                                                                 ataGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGaa                                                                                                                              >  2:339286/1‑91 (MQ=255)
                                                                                 ataGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGaa                                                                                                                              <  1:339286/91‑1 (MQ=255)
                                                                                         gACTTCACATCTTCAGCGATAGTCACATCCACCGTCTTCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt                                                            <  2:150542/149‑1 (MQ=255)
                                                                                               acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAg                                                      <  6:383584/149‑1 (MQ=255)
                                                                                               acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAg                                                      >  1:6773/1‑149 (MQ=255)
                                                                                               acaTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAg                                                      >  4:347611/1‑149 (MQ=255)
                                                                                                 aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttttttt                                                                                                                                       >  5:231964/1‑65 (MQ=25)
                                                                                                 aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttttttt                                                                                                                                       <  6:231964/66‑2 (MQ=25)
                                                                                                 aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                    >  4:185099/1‑149 (MQ=255)
                                                                                                          gATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCgg                                           <  4:237163/149‑1 (MQ=255)
                                                                                                            tAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGAAGTTTCATCGCGGCa                                         >  5:7397/1‑149 (MQ=255)
                                                                                                               tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGCACGTCTTGCAATAGTTATTGAACAAACTTTTCAGGATTTTGCGCAGTATCATCGCGGCACTc                                      >  1:100757/1‑149 (MQ=255)
                                                                                                               tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTc                                      >  6:81905/1‑149 (MQ=255)
                                                                                                               tCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTc                                      <  3:185099/149‑1 (MQ=255)
                                                                                                                    tCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACata                                                                                                                          <  8:181765/60‑1 (MQ=255)
                                                                                                                    tCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACata                                                                                                                          >  7:181765/1‑60 (MQ=255)
                                                                                                                    tCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc                                  >  3:271732/1‑148 (MQ=255)
                                                                                                                      cACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAt                               >  6:118946/1‑149 (MQ=255)
                                                                                                                        ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTTTTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt                             <  6:2175/149‑1 (MQ=255)
                                                                                                                          gTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt                                                            >  1:327525/1‑116 (MQ=255)
                                                                                                                          gTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt                                                            <  2:327525/116‑1 (MQ=255)
                                                                                                                          gTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGt                           <  3:225689/149‑1 (MQ=255)
                                                                                                                           tcatcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                          <  2:263058/149‑1 (MQ=255)
                                                                                                                             atcaCGACACAAAACAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAAAAGTTATTGAACATACTTTTCAGTATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTta                         <  2:255737/148‑1 (MQ=255)
                                                                                                                              tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtata                       >  1:77100/1‑149 (MQ=255)
                                                                                                                              tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCAACGCGGCACTCCGACCATTTGTtat                        >  2:39528/1‑148 (MQ=255)
                                                                                                                              tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCAACGCGGCACTCCGACCATTTGTtat                        <  1:39528/148‑1 (MQ=255)
                                                                                                                                aGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACa                     <  3:347611/149‑1 (MQ=255)
                                                                                                                                  gACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                          >  2:317466/1‑142 (MQ=255)
                                                                                                                                  gACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                          <  1:317466/142‑1 (MQ=255)
                                                                                                                                   acacAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgt                  <  2:213335/149‑1 (MQ=255)
                                                                                                                                     acaAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAGCATTTGTTATACAGgtct                >  2:360926/1‑147 (MQ=255)
                                                                                                                                        aaaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGGGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa              >  6:346132/1‑149 (MQ=255)
                                                                                                                                        aaaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa              >  5:72461/1‑149 (MQ=255)
                                                                                                                                        aaaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa              >  8:209488/1‑149 (MQ=255)
                                                                                                                                         aaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCCCTCCGACCATTTGTTATACAGGTGTTAt             >  6:96030/1‑149 (MQ=255)
                                                                                                                                         aaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt             >  7:67958/1‑149 (MQ=255)
                                                                                                                                         aaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt             >  7:261190/1‑149 (MQ=255)
                                                                                                                                          aaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt            >  8:411326/1‑149 (MQ=255)
                                                                                                                                            aaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTg           <  1:40122/148‑1 (MQ=255)
                                                                                                                                            aaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTg           >  2:40122/1‑148 (MQ=255)
                                                                                                                                                   ccGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTg     <  8:419963/147‑1 (MQ=255)
                                                                                                                                                    cGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTgcgc  >  1:292927/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGC  >  NC_000913/1987710‑1988005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: