New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 421984745 (1.050)6 (0.150) 6/218 3.8 13.4% coding (1252/1737 nt) aceK isocitrate dehydrogenase kinase/phosphatase
?NC_000913 = 4219854 37 (0.960)coding (1259/1737 nt) aceK isocitrate dehydrogenase kinase/phosphatase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCA  >  NC_000913/4219706‑4219991
                                                                                                                                                    |                                                                                                                                         
gACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGt                                                                                                                                           <  1:187749/149‑1 (MQ=255)
 acacCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTg                                                                                                                                          <  2:200799/149‑1 (MQ=255)
     ccAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCg                                                                                                                                       <  6:119532/148‑1 (MQ=255)
     ccAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCg                                                                                                                                       >  5:119532/1‑148 (MQ=255)
     ccAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGc                                                                                                                                      >  2:40141/1‑149 (MQ=255)
     ccAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGc                                                                                                                                      >  4:28550/1‑149 (MQ=255)
               gAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctg                                                                                                                            >  4:24010/1‑149 (MQ=255)
               gAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATct                                                                                                                             <  4:109108/148‑1 (MQ=255)
                 aaaCTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggc                                                                                                                          >  5:122674/1‑149 (MQ=255)
                  aaCTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggct                                                                                                                         <  3:28550/149‑1 (MQ=255)
                        gtgCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAAc                                                                                                                    <  6:122674/148‑1 (MQ=255)
                         tgCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACaa                                                                                                                  <  1:40141/149‑1 (MQ=255)
                                 aaGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAg                                                                                                           <  4:26499/148‑1 (MQ=255)
                                           tttCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCTATCTTTATATTGAGCGGCGGATGGTGCCGCTCaa                                                                                                                                  >  7:181679/1‑115 (MQ=255)
                                           tttCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCTATCTTTATATTGAGCGGCGGATGGTGCCGCGCaa                                                                                                                                  <  8:181679/115‑1 (MQ=255)
                                             tCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggc                                                                                                                          >  6:52043/1‑121 (MQ=255)
                                             tCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggc                                                                                                                          <  5:52043/121‑1 (MQ=255)
                                                         aTGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTga                                                                                  <  3:24010/149‑1 (MQ=255)
                                                          tGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATAGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaa                                                                                 <  5:129532/149‑1 (MQ=255)
                                                            gAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaag                                                                                >  7:155728/1‑148 (MQ=255)
                                                                 aCTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATAc                                                                           <  8:155728/148‑1 (MQ=255)
                                                                   tGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaa                                                                                 <  8:170900/140‑1 (MQ=255)
                                                                   tGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaa                                                                                 >  7:170900/1‑140 (MQ=255)
                                                                           gAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGc                                                                                        >  6:21646/1‑125 (MQ=255)
                                                                           gAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGc                                                                                        <  5:21646/125‑1 (MQ=255)
                                                                              gcagcGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGt                                                                                                         >  3:121728/1‑105 (MQ=255)
                                                                              gcagcGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGt                                                                                                         <  4:121728/105‑1 (MQ=255)
                                                                                 gcGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTaa                                                                      <  5:162593/137‑1 (MQ=255)
                                                                                 gcGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTaa                                                                      >  6:162593/1‑137 (MQ=255)
                                                                                      aaaaaTCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTg                                                     <  5:213676/149‑1 (MQ=255)
                                                                                                       gCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAAt                                                                             <  3:114495/108‑1 (MQ=255)
                                                                                                       gCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAAt                                                                             >  4:114495/1‑108 (MQ=255)
                                                                                                                     aTTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGGACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaa                                                                                 <  8:80536/90‑1 (MQ=255)
                                                                                                                     aTTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGGACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaa                                                                                 >  7:80536/1‑90 (MQ=255)
                                                                                                                     aTTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGc                              <  2:234416/141‑1 (MQ=255)
                                                                                                                     aTTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGc                              >  1:234416/1‑141 (MQ=255)
                                                                                                                     aTTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCt                      >  3:46610/1‑149 (MQ=255)
                                                                                                                         gCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTTGCTCGAACAAGTGGATGGTCAGCAGTTGCGCGACGACATTCAAGAAGACGGTAACGCTATACTCCAGCTTGCCGCTGCTAACATTATCCCTGGCGACATGCAGtt                   >  6:198163/1‑148 (MQ=255)
                                                                                                                              cTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTa                                            <  7:185626/118‑1 (MQ=255)
                                                                                                                              cTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTa                                            >  8:185626/1‑118 (MQ=255)
                                                                                                                                   tatTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCg        <  4:46610/149‑1 (MQ=255)
                                                                                                                                     ttGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCgg       <  4:177081/148‑1 (MQ=255)
                                                                                                                                     ttGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGgt      >  3:177081/1‑149 (MQ=255)
                                                                                                                                                    tGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCa  >  6:131307/1‑138 (MQ=255)
                                                                                                                                                    tGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCa  <  5:131307/138‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
GACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCA  >  NC_000913/4219706‑4219991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.