New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 42776227 (0.900)6 (0.210) 6/208 2.3 16.6% coding (116/1848 nt) secD SecYEG protein translocase auxillary subunit
?NC_000913 = 427779 35 (1.240)coding (133/1848 nt) secD SecYEG protein translocase auxillary subunit
Rejected: Frequency below/above cutoff threshold.

TAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAA  >  NC_000913/427634‑427913
                                                                                                                                                 |                                                                                                                                      
tAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGc                                                                                                                                       <  4:69892‑M2/147‑2 (MQ=255)
                                                       gtggtgATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACtcc                                                                              <  3:69892/149‑3 (MQ=255)
                                                        tggtgATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAg                                                                                                                 >  1:54652/1‑113 (MQ=255)
                                                        tggtgATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAg                                                                                                                 <  2:54652/113‑1 (MQ=255)
                                                                       gTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGt                                                                                                    <  4:19706/111‑1 (MQ=255)
                                                                       gTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGt                                                                                                    >  3:19706/1‑111 (MQ=255)
                                                                        tCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTa                                                                                                  >  1:117094/1‑112 (MQ=255)
                                                                        tCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTa                                                                                                  <  2:117094/112‑1 (MQ=255)
                                                                             tgTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAagag                                                        >  2:133363/1‑149 (MQ=255)
                                                                                    gcTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGtt                                                                                                   >  1:115601/1‑99 (MQ=255)
                                                                                    gcTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGtt                                                                                                   <  2:115601/99‑1 (MQ=255)
                                                                                    gcTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACaagaag                                                                                           <  3:45379/107‑1 (MQ=255)
                                                                                    gcTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACaagaag                                                                                           >  4:45379/1‑107 (MQ=255)
                                                                                     cTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAAc                                                                                 <  8:42296/116‑1 (MQ=255)
                                                                                     cTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAAc                                                                                 >  7:42296/1‑116 (MQ=255)
                                                                                        cccAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGaaaa                                                                                       >  8:17506/1‑107 (MQ=255)
                                                                                        cccAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGaaaa                                                                                       <  7:17506/107‑1 (MQ=255)
                                                                                          cAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTg                                           >  1:93280/1‑149 (MQ=255)
                                                                                            aCCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAg                                                                                                                 >  6:92108/1‑77 (MQ=255)
                                                                                            aCCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAg                                                                                                                 <  5:92108/77‑1 (MQ=255)
                                                                                               tGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGc                                                                              <  8:72148/109‑1 (MQ=255)
                                                                                               tGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGc                                                                              >  7:72148/1‑109 (MQ=255)
                                                                                                     gTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGa                                 <  1:133363/148‑1 (MQ=255)
                                                                                                                       aGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGtt               <  2:93280/148‑1 (MQ=255)
                                                                                                                        gATCACTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagcagaccgatgacaatcaccacgatcagcatga                                                   >  2:115116‑M2/1‑26 (MQ=255)
                                                                                                                        gATCACTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagcagaccgatgacaatcaccacgatcagcatga                                                   <  1:115116‑M2/111‑86 (MQ=255)
                                                                                                                         aTCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCtt                                    >  5:148148/1‑125 (MQ=255)
                                                                                                                         aTCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCtt                                    <  6:148148/125‑1 (MQ=255)
                                                                                                                          tCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGtt                                                                                                   >  5:100895/1‑61 (MQ=255)
                                                                                                                          tCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGtt                                                                                                   <  6:100895/61‑1 (MQ=255)
                                                                                                                            aCTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagcagaccgatgacaatcaccacgatcagcatgacgtacttccacaaa                                     <  4:136392‑M2/121‑100 (MQ=255)
                                                                                                                            aCTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagcagaccgatgacaatcaccacgatcagcatgacgtacttccacaaa                                     >  3:136392‑M2/1‑22 (MQ=255)
                                                                                                                              tGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagca                                                                                 >  2:137480‑M2/1‑20 (MQ=255)
                                                                                                                              tGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagca                                                                                 <  1:137480‑M2/75‑56 (MQ=255)
                                                                                                                                 tGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGa                                 >  1:20353/1‑120 (MQ=255)
                                                                                                                                 tGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGa                                 <  2:20353/120‑1 (MQ=255)
                                                                                                                                              gccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTgc                                          >  3:116362/1‑98 (MQ=255)
                                                                                                                                              gccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTgc                                          <  4:116362/98‑1 (MQ=255)
                                                                                                                                              gccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGaa  >  7:14613/1‑138 (MQ=255)
                                                                                                                                              gccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGaa  <  8:14613/138‑1 (MQ=255)
                                                                                                                                               ccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTgc             <  1:155134/126‑1 (MQ=255)
                                                                                                                                               ccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTgc             >  2:155134/1‑126 (MQ=255)
                                                                                                                                                 |                                                                                                                                      
TAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAA  >  NC_000913/427634‑427913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.