| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,260,901 | 0 | C | T | 30.4% | 26.2 / 14.1 | 24 | intergenic (‑98/+27) | dauA/prs | C4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (9/7); new base T (4/3); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCT > NC_000913/1260760‑1261044 | agagCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCgg > 1:137959/1‑149 (MQ=255) gCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCgg < 2:137959/149‑1 (MQ=255) aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt > 3:41201/1‑92 (MQ=255) aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt < 4:41201/92‑1 (MQ=255) aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacga < 8:20387/137‑1 (MQ=255) aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacga > 7:20387/1‑137 (MQ=255) gCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa > 1:78634/1‑88 (MQ=255) gCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa < 2:78634/88‑1 (MQ=255) aTAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg > 1:17271/1‑130 (MQ=255) aTAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg < 2:17271/130‑1 (MQ=255) aTAGGTCATACAAATGGATATTACAGACAAAAAAACACGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATg > 5:96287/1‑83 (MQ=255) aTAGGTAATACAAATGGATATTACAGACAAAAAATCCCGCGGGAGCGGGTCTTTGAGGCGGGTTCGATTAGTGTTCGTACATg < 6:96287/83‑1 (MQ=255) aCAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt > 3:87053/1‑136 (MQ=255) aCAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt < 4:87053/136‑1 (MQ=255) atatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAg > 3:135337/1‑149 (MQ=255) tatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGt > 3:45924/1‑149 (MQ=255) tcaaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc > 4:103111/3‑118 (MQ=255) tcaaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc < 3:103111/116‑1 (MQ=255) aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt < 4:135337/149‑1 (MQ=255) caaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttga > 1:117707/2‑145 (MQ=255) caaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttga < 2:117707/144‑1 (MQ=255) aaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa < 7:55380/117‑1 (MQ=255) aaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa > 8:55380/1‑117 (MQ=255) aaagaCCCGCTGCGGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa > 3:96826/1‑149 (MQ=255) ccgccgCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCt > 7:43564/1‑148 (MQ=255) | AGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCT > NC_000913/1260760‑1261044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |