Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,548,5710GT31.2% 15.7 / 10.5 16T500T (ACC→ACAmalQ4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base G (10/1);  new base T (0/5);  total (10/6)
Fisher's exact test for biased strand distribution p-value = 1.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAA  >  NC_000913/3548445‑3548706
                                                                                                                              |                                                                                                                                       
cGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCAc                                                                                                                         <  7:137890/143‑1 (MQ=255)
cGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCAc                                                                                                                         >  8:137890/1‑143 (MQ=255)
                        tttCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCAc                                                                                                                         >  6:161613/1‑119 (MQ=255)
                                                gTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAAACACCATACAGCGATGACGTTTACTTTCGAGTGCCAGCATCGAGagaaga                                                                    >  7:55063/1‑148 (MQ=255)
                                                    tCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGATGCGCAGCTTACCGACAATCTCTACCGGTACGGTAACGAGATCTTCACCAATCACCATACAGCGATGCAGTTTACTTTCGAGTGCCAGAATCTAGAGAAGatcagg                                                               >  7:262/1‑147 (MQ=255)
                                                               aTAGAGCACTTTGTAAGAGTACACACCTCTGCTGCGCAGATTTTTGTTTTTATTTATAGGGAGTGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGt                                                    <  8:55063/149‑1 (MQ=255)
                                                                        tttGTAAGAGTACACACCGCTGCTGCGCAGCTTATTTTTAATATTTATAGGTAGTGTACCGAGATCTTCACCAATCAc                                                                                                                  <  2:69639/78‑1 (MQ=255)
                                                                        tttGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCAc                                                                                                                  >  1:69639/1‑78 (MQ=255)
                                                                             aaGAGTACACACCGCTGCTGCGCAGCTTATTTTTTTTATTTATAGGTAGTGTACCGAGATCTTCACCAATCACCATACAGCg                                                                                                         <  2:28962/82‑1 (MQ=255)
                                                                             aaGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCg                                                                                                         >  1:28962/1‑82 (MQ=255)
                                                                                     acacCGCTGCTGCGCAGCTTATTTTTAATATTTATAGGTAGTGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGGCCTGATCTGc                              <  4:10851/149‑1 (MQ=255)
                                                                                     acacCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTAAACAATCACAATACAGAGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATACACCGGATAGTGAACATACGCTCCCTGATCAGc                              >  3:10851/1‑149 (MQ=255)
                                                                                          gctgctGCGCAGCTTATTTATTATATTTATAGGTAGTGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCAc                                                            <  8:124489/114‑1 (MQ=255)
                                                                                          gctgctGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCAc                                                            >  7:124489/1‑114 (MQ=255)
                                                                                                                 tctcTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACaaa  >  2:84326/1‑149 (MQ=255)
                                                                                                                     tACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTc                                                                                          >  5:133805/1‑57 (MQ=255)
                                                                                                                              |                                                                                                                                       
CGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTACGGTACCGAGATCTTCACCAATCACCATACAGCGATGACGTTTACTTTCGAGTGCCAGAATCGAGAGCAGATCATCCACCGGATAGTGAACATACGCGCCCTGATCTGCCGTCTCGCCATACGGTATCCACCACAAA  >  NC_000913/3548445‑3548706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: