Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 574,486:1 +C 6.0% intergenic (+147/+43) quuD → / ← nmpC DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913574,4861.C 6.0% 350.0 / 18.3 83intergenic (+147/+43)quuD/nmpCDLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
Reads supporting (aligned to +/- strand):  ref base . (40/38);  new base C (3/2);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGT  >  NC_000913/574339‑574634
                                                                                                                                                    |                                                                                                                                                    
atatGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTgg                                                                                                                                                     >  5:369166/1‑149 (MQ=255)
   tGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATAAGTTGGGGa                                                                                                                                                  <  2:156752/149‑1 (MQ=255)
         ttGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAAc                                                                                                                                            <  6:289369/149‑1 (MQ=255)
            tttACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTGCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCaaaggc                                                                                                                                         >  2:272480/1‑145 (MQ=255)
                cTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTccc                                                                                                                                     >  6:101852/1‑149 (MQ=255)
                 tAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAAAAGAAGGTTTATATAGTTAGAAGAAATGTTGTGATTAGATAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCg                                                                                                                                    >  7:293064/1‑149 (MQ=255)
                           taAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCt                                                                                                                          >  2:233051/1‑149 (MQ=255)
                            aaaaaTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCtt                                                                                                                         >  2:201879/1‑149 (MQ=255)
                            aaaaaTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCt                                                                                                                          <  8:333015/148‑1 (MQ=255)
                              aaaTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCtttt                                                                                                                       >  1:314867/1‑149 (MQ=255)
                               aaTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCttttt                                                                                                                      <  1:201879/149‑1 (MQ=255)
                               aaTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCttttt                                                                                                                      >  6:267067/1‑149 (MQ=255)
                                attgggCGCTGAGACGCCCACAAAAATAAAGGGTAATATATAACAGAAGGTTTATTTAGTTAGAACCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTc                                                                                                                     <  8:293064/146‑1 (MQ=255)
                                aTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTTAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTc                                                                                                                     <  1:284664/149‑1 (MQ=255)
                                aTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTc                                                                                                                     <  1:233051/149‑1 (MQ=255)
                                aTGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTGGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTc                                                                                                                     <  1:284666/149‑1 (MQ=255)
                                   gggCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGTCAACGCCTCCCGCAACATATCTTTTTCGt                                                                                                                   >  4:213424/1‑148 (MQ=255)
                                    ggCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTaa                                                                                                                 >  2:40628/1‑149 (MQ=255)
                                     gCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAAt                                                                                                                <  6:369166/149‑1 (MQ=255)
                                      cGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCACGGGTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATc                                                                                                               <  5:85050/149‑1 (MQ=255)
                                        ttGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCAGCAACATATCTTTTTCGTAAt                                                                                                                >  1:98527/1‑146 (MQ=255)
                                         tGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTg                                                                                                                                                       <  4:71880/107‑1 (MQ=255)
                                         tGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTg                                                                                                                                                       >  3:71880/1‑107 (MQ=255)
                                           agaCGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGAt                                                                                                           <  1:284945/148‑1 (MQ=255)
                                               gCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGaa                                                                                                      >  3:182795/1‑149 (MQ=255)
                                               gCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGaa                                                                                                      <  4:327467/149‑1 (MQ=255)
                                               gCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGaa                                                                                                      <  8:36359/149‑1 (MQ=255)
                                                 ccccAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACt                                                                                                    <  2:314867/149‑1 (MQ=255)
                                                    cAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTgg                                                                                                  >  3:59152/1‑148 (MQ=255)
                                                     aaaaaTAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTTGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGt                                                                                                 >  5:238431/1‑148 (MQ=255)
                                                            aaGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGa                                                                                         <  7:94071/149‑1 (MQ=255)
                                                            aaGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGa                                                                                         <  4:177960/149‑1 (MQ=255)
                                                              gggTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGATTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGAcc                                                                                       <  3:213424/149‑1 (MQ=255)
                                                                   atatatAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACa                                                                                   <  3:230805/148‑1 (MQ=255)
                                                                      tataACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGTCTTGAGGGAGACACTTATATGTTGGGGAGGCAACGCCTACAGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCaa                                                                               >  3:244752/1‑149 (MQ=255)
                                                                      tataACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCaa                                                                               <  4:12983/149‑1 (MQ=255)
                                                                            aGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCa                                                                                <  1:303007/142‑1 (MQ=255)
                                                                            aGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCa                                                                                >  2:303007/1‑142 (MQ=255)
                                                                            aGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGt                                                                         >  4:136495/1‑149 (MQ=255)
                                                                             gAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGtc                                                                        <  5:267067/149‑1 (MQ=255)
                                                                                 gTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGtcatca                                                                    <  8:46146/149‑1 (MQ=255)
                                                                                 gTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGtcat                                                                      >  5:152340/1‑147 (MQ=255)
                                                                                 gTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGtcat                                                                      >  6:181935/1‑147 (MQ=255)
                                                                                     atataGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTg                                                                 <  3:136495/148‑1 (MQ=255)
                                                                                      tataGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCACCATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCt                                                               >  2:264343/1‑149 (MQ=255)
                                                                                       ataGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCt                                                               >  5:61824/1‑148 (MQ=255)
                                                                                          gTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTaca                                                           >  8:110498/1‑149 (MQ=255)
                                                                                           ttAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTacac                                                          >  2:326317/1‑149 (MQ=255)
                                                                                           ttAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGAATCGATGTCATAAGTGCTTagac                                                          >  5:22772/1‑149 (MQ=255)
                                                                                              gAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACa                                                                                   >  4:151667/1‑121 (MQ=255)
                                                                                              gAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACAcc                                                         >  3:393211/1‑147 (MQ=255)
                                                                                               aaGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGCAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACAGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGac                                                      >  7:73053/1‑148 (MQ=255)
                                                                                                  cAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTa                                                                                                         <  3:129804/95‑1 (MQ=255)
                                                                                                  cAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTa                                                                                                         >  4:129804/1‑95 (MQ=255)
                                                                                                       ttGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAg                                              <  4:182795/149‑1 (MQ=255)
                                                                                                         gtgCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCGACGATGTCATCAGTGCTTACACCGAGTGCTTTAgg                                             >  5:338112/1‑148 (MQ=255)
                                                                                                               cTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGAt                                                                           <  5:167435/112‑1 (MQ=255)
                                                                                                               cTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGAt                                                                           >  6:167435/1‑112 (MQ=255)
                                                                                                               cTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGt                                       <  5:101852/148‑1 (MQ=255)
                                                                                                                 aaaGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGt                                                                  <  3:111698/119‑1 (MQ=255)
                                                                                                                 aaaGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGt                                                                  >  4:111698/1‑119 (MQ=255)
                                                                                                                   aGGAAGTGTCTTGAGGGAGCAACTTATATGTTGGGGAGGCAACGACGACCGCAACATATCTTTTTCGTAATCATATTATAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGaa                                  <  6:22772/149‑1 (MQ=255)
                                                                                                                         tGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGa                                                                            <  8:170602/101‑1 (MQ=255)
                                                                                                                         tGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGa                                                                            >  7:170602/1‑101 (MQ=255)
                                                                                                                           gCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATa                                >  4:321923/1‑143 (MQ=255)
                                                                                                                           gCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATa                                <  3:321923/143‑1 (MQ=255)
                                                                                                                           gCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgg                           <  5:181935/148‑1 (MQ=255)
                                                                                                                           gCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgg                           <  1:326317/148‑1 (MQ=255)
                                                                                                                                aGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAAt                     <  7:110498/149‑1 (MQ=255)
                                                                                                                                     gCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAACCCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAAttcttc                >  5:235134/1‑149 (MQ=255)
                                                                                                                                     gCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACATCAACGATGTCATCAGTGCTTACACCGAGGGCTTTAGGGAAGGTGCGAATAAGCGGGGAAAttcttc                <  4:182729/149‑1 (MQ=255)
                                                                                                                                      aaaaagatatGTTGCGGGAGGCGTTGCCTCCCCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGaaa                      >  2:372052/7‑142 (MQ=38)
                                                                                                                                      aaaaagatatGTTGCGGGAGGCGTTGCCTCCCCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGaaa                      <  1:372052/136‑1 (MQ=38)
                                                                                                                                       aaaagatatGTTGCGGGAGGCGTTGCCTCCCCAACATATCTTTTTCGTAATCAGATGAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGTGAAGGTGCGAATAAGCTGGGAAATTATtctc              >  8:310382/6‑149 (MQ=37)
                                                                                                                                        aCTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg             >  6:269180/1‑149 (MQ=255)
                                                                                                                                         cTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgg                           <  3:232434/134‑1 (MQ=255)
                                                                                                                                         cTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgg                           >  4:232434/1‑134 (MQ=255)
                                                                                                                                          ttATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc           >  7:374460/1‑149 (MQ=255)
                                                                                                                                           gatatGTTGCGGGAGGCGTTGCCTCCCCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTtctc              >  7:129903/2‑145 (MQ=38)
                                                                                                                                           gatatGTTGCGGGAGGCGTTGCCTCCCCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTtctc              <  8:129903/144‑1 (MQ=38)
                                                                                                                                            atatGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc           <  4:393211/147‑1 (MQ=255)
                                                                                                                                            atatGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt          >  6:298804/1‑148 (MQ=255)
                                                                                                                                             tatGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGa        >  6:44545/1‑149 (MQ=255)
                                                                                                                                               tGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACt      <  3:137172/149‑1 (MQ=255)
                                                                                                                                                gTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACt      >  2:256582/1‑148 (MQ=255)
                                                                                                                                                gTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGATGACt      >  6:126189/1‑148 (MQ=255)
                                                                                                                                                   ggggAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGt  >  3:225882/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTGGGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGT  >  NC_000913/574339‑574634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: