Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	1,806,703	Δ3 bp	8.1%	coding (334‑336/930 nt)	pfkB →	6‑phosphofructokinase II

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1806702	69 (0.760)	6 (0.070)	6/260	NT	8.1%	coding (333/930 nt)	pfkB	6‑phosphofructokinase II
	?	NC_000913	1806706 =	68 (0.750)					coding (337/930 nt)	pfkB	6‑phosphofructokinase II

GACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAAGGGATCCG  >  NC_000913/1806561‑1806854                                                                                                                                                  |                                                                                                                                                     gACCGGCGAACACCTGGTTTCACTGTTGGCGGAGGAAAATGTCCCCGTCGGTACTGTAGAAGCCAAAGACTGGACCCGGCATAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTaa                                                                                                                                                   <  7:158433/149‑1 (MQ=255)  aCCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTaaa                                                                                                                                                  <  2:12269/149‑1 (MQ=255)    cGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAAt                                                                                                                                                 >  8:244195/1‑148 (MQ=255)    cGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATg                                                                                                                                                <  7:244195/149‑1 (MQ=255)     ggCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGa                                                                                                                                               <  5:92199/149‑1 (MQ=255)      gCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGTATTTACACTTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGaa                                                                                                                                              <  6:371346/149‑1 (MQ=255)      gCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGaa                                                                                                                                              <  8:403502/149‑1 (MQ=255)         aaCAGCTGGTTTCTATGTTGGCGGATGAAAATGTGCGCGTCGGTACTGTAGATGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGAt                                                                                                                                           <  6:2809/149‑1 (MQ=255)           cacCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGAt                                                                                                                                           >  2:336621/1‑147 (MQ=255)            acCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAg                                                                                                                                        >  5:408801/1‑149 (MQ=255)             ccTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCACGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGt                                                                                                                                       <  8:319598/149‑1 (MQ=255)              cTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGATACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCTGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATTCAGATGAGtt                                                                                                                                      >  5:167338/1‑149 (MQ=255)                    tCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCTTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGcc                                                                                                                                 <  4:87598/148‑1 (MQ=255)                      aCTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGc                                                                                                                              <  1:41963/149‑1 (MQ=255)                       cTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCt                                                                                                                             <  2:417926/149‑1 (MQ=255)                        tGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCTCGGCATTAAATGAAGATGAGTTTCGCCAGCtt                                                                                                                            >  3:288660/1‑149 (MQ=255)                        tGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCtt                                                                                                                            >  8:58934/1‑149 (MQ=255)                          ttGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCtt                                                                                                                            <  8:64194/147‑1 (MQ=255)                          ttGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGa                                                                                                                          <  7:58934/149‑1 (MQ=255)                             gCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGaa                                                                                                                         >  5:80645/1‑147 (MQ=255)                             gCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAaga                                                                                                                       <  8:65023/149‑1 (MQ=255)                               ggATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGc                                                                                                                     <  2:207581/149‑1 (MQ=255)                                gATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGc                                                                                                                     >  2:62425/1‑148 (MQ=255)                                gATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGc                                                                                                                     >  6:280668/1‑148 (MQ=255)                                   gAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGt                                                                                                                 >  7:254126/1‑149 (MQ=255)                                     aaaTGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTc                                                                                                               <  5:313934/149‑1 (MQ=255)                                       aTGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTg                                                                                                             <  8:64731/149‑1 (MQ=255)                                             ccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATTCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAtt                                                                                                       <  1:275766/149‑1 (MQ=255)                                             ccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAtt                                                                                                       <  1:62425/149‑1 (MQ=255)                                             ccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAtt                                                                                                       <  5:280668/149‑1 (MQ=255)                                                   cTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATcc                                                                                                 >  7:326741/1‑149 (MQ=255)                                                    tACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCg                                                                                                >  6:174823/1‑149 (MQ=255)                                                      cTGTAGAAGCCAATGAGTGGACCAGGCAGAATTTACAAGTACTTGTGGAAGCTAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTGCTGGAAATTGAATCCg                                                                                                <  6:167338/147‑1 (MQ=255)                                                      cTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCgg                                                                                               <  8:326741/148‑1 (MQ=255)                                                       tGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCgggg                                                                                             >  8:110870/1‑149 (MQ=255)                                                           gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGATGAGCAAGTTCTGGAAATTGAATCCGGGGCCAt                                                                                         >  1:398429/1‑149 (MQ=255)                                                           gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCg                                                                                                >  3:101874/1‑142 (MQ=255)                                                           gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCg                                                                                                <  4:101874/142‑1 (MQ=255)                                                                cAAAGACTGCACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTgg                                                                                    >  2:369479/1‑149 (MQ=255)                                                                 aaaGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGt                                                                                   >  3:232504/1‑149 (MQ=255)                                                                 aaaGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGt                                                                                   >  1:224196/1‑149 (MQ=255)                                                                  aaGACTGGACCCGGCAGAATTTACACGTACATGGGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTc                                                                                  <  2:133066/149‑1 (MQ=255)                                                                   aGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTc                                                                                  >  2:423908/1‑148 (MQ=255)                                                                       tGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCAGGTCATAAg                                                                             >  5:206580/1‑149 (MQ=255)                                                                            ccGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGGATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCgg                                                                          >  3:241457/1‑147 (MQ=255)                                                                             cGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAg                                                                       <  6:80645/149‑1 (MQ=255)                                                                             acccggcagaatttacacgtacatgtggaagcaagcggtgagcagtatcgttttgttatgccaggcgcgTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAg                                                                       <  2:370153‑M2/80‑1 (MQ=255)                                                                              ggCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGGTTTGTTATGCCAGGCGCGGCATTAAATGAAGCTGAGTTTCGCCAGCTGGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGc                                                                      >  3:111984/1‑149 (MQ=255)                                                                               gCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGcc                                                                     >  2:197637/1‑149 (MQ=255)                                                                               ccggcagaatttacacgtacatgtggaagcaagcggtgagcagtatcgttttgttatgccaggcgcgTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGcc                                                                     >  7:228029‑M2/68‑149 (MQ=255)                                                                                cAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCt                                                                    <  8:254126/149‑1 (MQ=255)                                                                                          acGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGgt                                                          >  6:178968/1‑149 (MQ=255)                                                                                             tACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGgtgt                                                        >  8:246505/1‑148 (MQ=255)                                                                                               cATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGa                                                      <  7:246505/148‑1 (MQ=255)                                                                                                 tgtgGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGc                                                   >  7:319188/1‑149 (MQ=255)                                                                                                  gtgGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCt                                                  >  2:214469/1‑149 (MQ=255)                                                                                                    tgtggaagcaagcggtgagcagtatcgttttgttatgccaggcgcgTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTg                                                 >  6:227129‑M2/47‑148 (MQ=255)                                                                                                     gAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTgg                                                >  4:379047/1‑148 (MQ=255)                                                                                                        gaagcaagcggtgagcagtatcgttttgttatgccaggcgcgTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGaaa                                             <  5:227129‑M2/106‑1 (MQ=255)                                                                                                             cGGTCAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGGCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTaa                                       <  8:417451/149‑1 (MQ=255)                                                                                                                tGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAAcc                                     >  5:67654/1‑148 (MQ=255)                                                                                                                   gCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAAc                                 <  7:110870/149‑1 (MQ=255)                                                                                                                     aGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACt                                >  2:417869/1‑148 (MQ=255)                                                                                                                         tCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGCAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGATGGAAAAATTAACCCAACTGAttt                           >  8:422593/1‑149 (MQ=255)                                                                                                                          tatcgttttgttatgccaggcgcgTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTg                                                                                     <  7:306222‑M2/66‑1 (MQ=255)                                                                                                                          tatcgttttgttatgccaggcgcgTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTg                                                                                     >  8:306222‑M2/25‑90 (MQ=255)                                                                                                                          cGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTc                          >  1:355113/1‑149 (MQ=255)                                                                                                                           gTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGc                                                                                                                                  <  2:398236/44‑1 (MQ=255)                                                                                                                           gTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGc                                                                                                                                  >  1:398236/1‑44 (MQ=255)                                                                                                                           gTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTcc                         >  2:212369/1‑149 (MQ=255)                                                                                                                             tttGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGc                       >  5:98947/1‑149 (MQ=255)                                                                                                                                   aTGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTgcgc                  >  4:171215/1‑148 (MQ=255)                                                                                                                                       cAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCaaaaa             >  8:171230/1‑149 (MQ=255)                                                                                                                                       cAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCaaaaa             >  4:229912/1‑149 (MQ=255)                                                                                                                                       cAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCaaaaa             >  5:391204/1‑149 (MQ=255)                                                                                                                                           cgcgGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACaat         >  4:288044/1‑148 (MQ=255)                                                                                                                                               gCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAAGGGAt     <  7:171230/149‑1 (MQ=255)                                                                                                                                                 aTTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAAGGGATcc   >  7:347103/1‑149 (MQ=255)                                                                                                                                                  ttAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAAGGGATCCg  <  1:197637/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAAGGGATCCG  >  NC_000913/1806561‑1806854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.