Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4001645 4001649 5 111 [0] [0] 111 corA magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016440 (0.000)100 (1.100) 81/260 NT 100% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001650 = 0 (0.000)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

Only 100 of 111 total aligned reads displayed.

AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGG  >  NC_000913/4001503‑4001798
                                                                                                                                                   |                                                                                                                                                    
aatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatct                                                                                                                                                     >  3:301272‑M2/148‑149 (MQ=255)
  tgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                                   <  8:362263‑M2/4‑1 (MQ=255)
      ttatggatggatcttgtcgaaacggacgaagacgagcgactgctcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgttgctttgaagacgactacggcctgcatCTCcttct                                                                                                                                               <  3:16159‑M2/8‑1 (MQ=255)
        atggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttc                                                                                                                                             >  4:54256‑M2/140‑149 (MQ=255)
         tggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttc                                                                                                                                             <  8:150996‑M2/10‑1 (MQ=255)
           gattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttc                                                                                                                                          <  6:330344‑M2/13‑1 (MQ=255)
            attgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggacctggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttattct                                                                                                                                         >  5:52053‑M2/136‑149 (MQ=255)
            attgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttct                                                                                                                                         >  8:248776‑M2/136‑149 (MQ=255)
                atcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                                      <  8:196083‑M2/17‑1 (MQ=255)
                 tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                                      >  5:362375‑M2/131‑147 (MQ=255)
                 tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagactggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                                      >  1:239662‑M2/131‑147 (MQ=255)
                   ttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttaaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                                      >  8:40047‑M2/129‑145 (MQ=255)
                     gtcgaaccggacgacgacgagcgcctgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGaa                                                                                                                                    <  5:44888‑M2/19‑1 (MQ=255)
                     gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGa                                                                                                                                     <  1:380962‑M2/18‑1 (MQ=255)
                      tcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                                               <  4:110781‑M2/24‑1 (MQ=255)
                      tcgaaccggacgacgacgagagactgcgcgtacattctgatcttggccagagcctggcaagccgcccggaactggaagacatcgtagcttcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                                               <  2:59057‑M2/24‑1 (MQ=255)
                         aaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCGTCTGTGAAGATGCgg                                                                                                                             >  5:290590‑M2/123‑148 (MQ=255)
                          accggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGTGGaa                                                                                                                           <  3:14485‑M2/28‑1 (MQ=255)
                             ggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAt                                                                                                                        <  4:191919‑M2/31‑1 (MQ=255)
                             ggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAt                                                                                                                        <  1:282617‑M2/31‑1 (MQ=255)
                                  acgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGc                                                                                                                   <  7:207749‑M2/36‑1 (MQ=255)
                                  acgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGc                                                                                                                   <  3:285886‑M2/36‑1 (MQ=255)
                                   cgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaccccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGcc                                                                                                                  >  8:385468‑M2/113‑149 (MQ=255)
                                    gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaccccgcccggaactggaagacatcgcagcatcggcacggttctttgaagccgccgacggcctgagtCTCCTTATTCTTCTTTGAAGATGAGGAAGATCACGCCg                                                                                                                 >  1:347310‑M2/112‑149 (MQ=255)
                                    gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaccccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                                 >  5:8509‑M2/112‑149 (MQ=255)
                                    gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                                 >  5:181557‑M2/112‑149 (MQ=255)
                                         gcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAAc                                                                                                            >  1:285073‑M2/107‑149 (MQ=255)
                                            actgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTc                                                                                                          >  7:289833‑M2/104‑148 (MQ=255)
                                                cgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACt                                                                                                      <  8:289833‑M2/49‑1 (MQ=255)
                                                 gcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgt                                                                                                    <  2:402905‑M2/51‑1 (MQ=255)
                                                  cgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttcaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                                                   <  7:40047‑M2/52‑1 (MQ=255)
                                                   gtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctccatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTgg                                                                                                  >  2:366272‑M2/97‑149 (MQ=255)
                                                     acaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCa                                                                                                >  6:81230‑M2/95‑149 (MQ=255)
                                                       aatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatctgcacgtttcttagaagacgacgacgacaagcatCTCCTTCAACTTCTTTGAAGATGCGCAAGATCAATCCGCTAActccaatgttgaaat                                                                                              >  2:110800‑M2/93‑143 (MQ=255)
                                                               cttggccagagcctggcaacccgcacggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGAGGAAGATCACGCCGGTAACTCCAATGTGGCATTTACCATcc                                                                                      >  6:319709‑M2/85‑149 (MQ=255)
                                                                ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCg                                                                                     >  5:133522‑M2/84‑149 (MQ=255)
                                                                    ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAt                                                                                 >  1:384557‑M2/80‑149 (MQ=255)
                                                                      agagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATgg                                                                               >  2:234920‑M2/78‑149 (MQ=255)
                                                                       gagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgt                                                                              >  4:23143‑M2/77‑149 (MQ=255)
                                                                           ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtcgtc                                                                          >  1:336519‑M2/73‑149 (MQ=255)
                                                                            tggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCACCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCt                                                                         >  8:352197‑M2/72‑149 (MQ=255)
                                                                             ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtc                                                                             >  3:74363‑M2/71‑144 (MQ=255)
                                                                             ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtc                                                                             <  4:74363‑M2/74‑1 (MQ=255)
                                                                               caaccggcccggaactggaagacatcgaagcatcggcacgtttcttgcaagacgacgactgcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                                      <  4:47265‑M2/81‑1 (MQ=255)
                                                                               caacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                                      >  1:5050‑M2/69‑149 (MQ=255)
                                                                                aacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                                      >  2:268675‑M2/68‑148 (MQ=255)
                                                                                  cccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAc                                                                   <  3:188666‑M2/84‑1 (MQ=255)
                                                                                  cccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAc                                                                   <  1:268675‑M2/84‑1 (MQ=255)
                                                                                   ccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAc                                                                   >  2:71447‑M2/65‑148 (MQ=255)
                                                                                    cgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTActc                                                                 <  2:413990‑M2/86‑1 (MQ=255)
                                                                                     gcccggaactggaagacatcgaagcatctgcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGc                                                                                                 <  6:123294‑M2/54‑1 (MQ=255)
                                                                                     gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGc                                                                                                 >  5:123294‑M2/63‑116 (MQ=255)
                                                                                       ccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                                              <  5:319709‑M2/89‑1 (MQ=255)
                                                                                        cggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcg                                                             <  5:81230‑M2/90‑1 (MQ=255)
                                                                                         ggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgt                                                            <  4:301272‑M2/91‑1 (MQ=255)
                                                                                             ctggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCATCTTTGAAGATGCGGAAGATCACGCCGGGAAAACCACTGTGGCATTTACCATCCGTGATGGTCGTATTTTTACTCTgcgtga                                                          >  6:50903‑M2/55‑147 (MQ=255)
                                                                                                  agacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTGCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                                   <  3:110191‑M2/100‑1 (MQ=255)
                                                                                                    acatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                                              <  6:197057‑M2/89‑1 (MQ=255)
                                                                                                    acatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                                              >  5:197057‑M2/48‑136 (MQ=255)
                                                                                                       tcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGcc                                              >  4:250418‑M2/45‑149 (MQ=255)
                                                                                                         gaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGcc                                              <  2:239662‑M2/105‑1 (MQ=255)
                                                                                                          aagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAtt                                                                                              >  3:239067‑M2/42‑98 (MQ=255)
                                                                                                          aagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAtt                                                                                              <  4:239067‑M2/57‑1 (MQ=255)
                                                                                                          aagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGc                                           >  7:41598‑M2/42‑149 (MQ=255)
                                                                                                            gcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCtt                                         >  2:228892‑M2/40‑149 (MQ=255)
                                                                                                              atcggcaggtttcttttaagacgacgacggcctgcatCTCCTTCTTATTCTTTGATGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTa                                                                    <  6:72885‑M2/83‑1 (MQ=255)
                                                                                                              atcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGCTAACTCCACTGTGGCATTTACCATCCCTGATGGTCGTCTGTTTa                                                                    >  5:72885‑M2/38‑120 (MQ=255)
                                                                                                               tcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                                   >  5:306344‑M2/37‑136 (MQ=255)
                                                                                                               tcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                                   <  6:306344‑M2/100‑1 (MQ=255)
                                                                                                               tcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTc                                      <  1:228892‑M2/113‑1 (MQ=255)
                                                                                                               tcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTc                                      <  1:71447‑M2/113‑1 (MQ=255)
                                                                                                                  gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGt                                                                                    <  6:214174‑M2/67‑1 (MQ=255)
                                                                                                                  gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGt                                                                                    >  5:214174‑M2/34‑100 (MQ=255)
                                                                                                                    acgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTg                                 >  5:248636‑M2/32‑149 (MQ=255)
                                                                                                                    acgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTg                                 <  2:285073‑M2/118‑1 (MQ=255)
                                                                                                                      gtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTa                               <  6:133522‑M2/120‑1 (MQ=255)
                                                                                                                      gtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTa                               >  2:102131‑M2/30‑149 (MQ=255)
                                                                                                                       tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                                              >  3:218065‑M2/29‑117 (MQ=255)
                                                                                                                       tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTTTAt                              >  8:398371‑M2/29‑149 (MQ=255)
                                                                                                                       tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACCCCGGTAACTCCACTGTGGCATTTACCATCGGTGATGGTCGTCTGTTTACTCTgc                                                              <  4:218065‑M2/89‑1 (MQ=255)
                                                                                                                         tctttgcagacgacgacggcctgcttCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATc                             <  5:50903‑M2/122‑1 (MQ=255)
                                                                                                                          ctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCg                            <  6:52053‑M2/123‑1 (MQ=255)
                                                                                                                             tgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa                          <  6:362375‑M2/125‑1 (MQ=255)
                                                                                                                             tgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAt                         <  7:398371‑M2/126‑1 (MQ=255)
                                                                                                                               aagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGc                       <  6:8509‑M2/128‑1 (MQ=255)
                                                                                                                                agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGt                     >  7:364449‑M2/20‑149 (MQ=255)
                                                                                                                                    gacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGccc                 >  1:419132‑M2/16‑149 (MQ=255)
                                                                                                                                     acgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCg                <  3:23143‑M2/135‑1 (MQ=255)
                                                                                                                                       gacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCg                >  3:219985‑M2/13‑147 (MQ=255)
                                                                                                                                       gacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTa              <  7:248776‑M2/137‑1 (MQ=255)
                                                                                                                                        acggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAg             <  6:181557‑M2/138‑1 (MQ=255)
                                                                                                                                         cggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGc            >  8:65602‑M2/11‑149 (MQ=255)
                                                                                                                                         cggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCAATGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGc            >  4:390620‑M2/11‑149 (MQ=255)
                                                                                                                                          ggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGcc           <  2:5050‑M2/140‑1 (MQ=255)
                                                                                                                                              tgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTc       >  3:78570‑M2/6‑149 (MQ=255)
                                                                                                                                               gcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCt                                          >  8:129083‑M2/5‑113 (MQ=255)
                                                                                                                                               gcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTCTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTCCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTc       >  5:65241‑M2/5‑148 (MQ=255)
                                                                                                                                                  tCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTTCCCGTAGCCAGTCGATg   >  1:64216‑M2/2‑149 (MQ=255)
                                                                                                                                                   cTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATgg  >  1:148221/1‑149 (MQ=255)
                                                                                                                                                   cTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGGCGATgg  >  5:4763/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGG  >  NC_000913/4001503‑4001798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: