Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,231,052 G→A 5.5% V96M (GTG→ATG)  umuD → translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,231,0520GA 5.5% 228.5 / 2.5 73V96M (GTG→ATG) umuDtranslesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease
Reads supporting (aligned to +/- strand):  ref base G (28/41);  new base A (2/2);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

TCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTG  >  NC_000913/1230907‑1231192
                                                                                                                                                 |                                                                                                                                            
tCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAGGGTGGAATTAGTGACGGTGATTTACGGATTGTCGATAGCGCTATTACCGCCCGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTg                                                                                                                                            >  3:327218/1‑148 (MQ=255)
tCCAAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGACGATAGCGCTATTACCGCCAGAAATGGTGATATAGTCATCGATGCTGATGACGGCGAGTTTACGGTg                                                                                                                                            >  7:418090/1‑148 (MQ=255)
     gcgcGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGaaaaaa                                                                                                                                      <  8:415836/149‑1 (MQ=255)
       gcgACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAtt                                                                                                                                    <  7:102778/149‑1 (MQ=255)
       gcgACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAtt                                                                                                                                    <  4:209672/149‑1 (MQ=255)
       gcgACATACTTCTTCAAAGCCAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACGGCCAGCCATGGTTATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAAtt                                                                                                                                    <  4:95203/149‑1 (MQ=255)
          acttacttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCa                                                                                                                                 <  8:186587/149‑1 (MQ=255)
           cttacttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCaa                                                                                                                                <  4:159853/149‑1 (MQ=255)
              acttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTa                                                                                                                             >  7:76453/1‑149 (MQ=255)
               cttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTAc                                                                                                                            >  8:68595/1‑149 (MQ=255)
               cttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTAc                                                                                                                            <  8:379768/149‑1 (MQ=255)
                ttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACg                                                                                                                           >  8:173772/1‑149 (MQ=255)
                ttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGATGAAAAAATTGCAACTACg                                                                                                                           >  8:311801/1‑149 (MQ=255)
                ttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGATGAAAAAATTGCAACTACg                                                                                                                           >  8:305997/1‑149 (MQ=255)
                  cGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGcc                                                                                                                         >  3:206129/1‑149 (MQ=255)
                   gTCAAAGCAAGTGGTGAGTCTATGATTGCTGGTGGACTTAGTGACGGTGAATTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCGGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACCACGccc                                                                                                                        <  4:359/149‑1 (MQ=255)
                      aaaGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGa                                                                                                                      <  7:238927/148‑1 (MQ=255)
                      aaaGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGAc                                                                                                                     >  3:360590/1‑149 (MQ=255)
                        aGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACgg                                                                                                                   >  1:107269/1‑149 (MQ=255)
                         cctactggtgTTTCTTTTATTGTTGGTGGAATTAGTGACGGTGTTTTAGTGTTTGGCGATAGGGCTATTACTCCCAGCCATGGTGAAATTGTCAGCGCTGCTGTTGACGGCGAGTTTTCGGTGAAAAAATTGTTACTTCGCCCGACgg                                                                                                                   <  2:154334/143‑1 (MQ=255)
                          cAAGTGGTTATTATATGATTGATGGTGGAATTAGTGACGGTGATTTACTTATTGTCGATAGCGCTATTACCGCCAGCCTTGGTGATATTGTCTTCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTa                                                                                                                 <  2:107269/149‑1 (MQ=255)
                           aaGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCTTCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTAc                                                                                                                <  1:330343/149‑1 (MQ=255)
                              tggttATTCTATGATTGATGGTGGAATTAGTGAGGGTGTTTTACTGATTGTCGATAGGGCTTTTACCGCCAGCCATGGTTATATTGTCATCGCTGCTGTTGAGGGCGAGTTTACGGTGAAAAATTTGCAACTACGCCCGACGGTACAg                                                                                                              <  8:76453/148‑1 (MQ=255)
                              tggtgATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACTGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGc                                                                                                             >  3:243732/1‑149 (MQ=255)
                                gtgATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACTGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCt                                                                                                            >  3:105712/1‑148 (MQ=255)
                                  gATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAt                                                                                                         >  4:329265/1‑149 (MQ=255)
                                   aTTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTAtt                                                                                                        <  6:271192/149‑1 (MQ=255)
                                    ttCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACAGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTc                                                                                                       >  8:110029/1‑149 (MQ=255)
                                    ttCTATGATTGATGGGGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTc                                                                                                       <  1:42630/149‑1 (MQ=255)
                                     tCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGGTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTcc                                                                                                      >  7:137895/1‑149 (MQ=255)
                                      cTATGATTGTTGGTGGATTTAGTGACGGTGATTTACTGATTGTCGTAAGCTCTTTTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGAAACTACGCCCGACGGTACAGCTTATTcc                                                                                                      <  4:206129/148‑1 (MQ=255)
                                       tATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa                                                                                                    >  7:364828/1‑149 (MQ=255)
                                        aTGTTTGATGGTGGATTTAGTGAGTGTGATTTAGTTATTGTCGATAGCGTTTTTACCGCCAGCGATGGTGTTATTGTCATCGATGCGGTTGCCGGCGAGTTTACGGTGAAAAAATTGCAACTACCCCCGACGGTACAGCTTATTCCCa                                                                                                    <  8:418090/148‑1 (MQ=255)
                                         tgattgatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCa                                                                                                    <  2:250654/147‑1 (MQ=255)
                                              gatGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTTACGGCGATTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACa                                                                                              <  5:347582/148‑1 (MQ=255)
                                                     caaTTAGTGACGGTGATTTACTGATTGTCTATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTTAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTa                                                                                       <  6:153406/147‑1 (MQ=255)
                                                        ttAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCg                                                                                   <  7:181234/149‑1 (MQ=255)
                                                           gTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGcc                                                                                 >  3:77084/1‑148 (MQ=255)
                                                            tGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCa                                                                               >  1:245809/1‑149 (MQ=255)
                                                              aCGGTGATTTACTGATTGTCGATAGCTCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGAAGGCGAGTTTACGGTGAAAAAATTGCAAATACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCAt                                                                              >  6:181564/1‑148 (MQ=255)
                                                              aCGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTTAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCAtt                                                                             <  4:360590/149‑1 (MQ=255)
                                                                         cTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCagtag                                                                  >  1:51587/1‑149 (MQ=255)
                                                                         cTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCagta                                                                   <  4:77084/148‑1 (MQ=255)
                                                                          tGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCAATGAACAGCGCGTACTCGCCCATTACCATCagtagt                                                                 >  6:353301/1‑149 (MQ=255)
                                                                          tGATTGTCGATAGCGCTATTACCCCCAGCCATGGTGATATTGTCATCGCTGCTGTTGCCGGCGAGTTAACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACACCGCGTACTCGCCCATAACCATCataagt                                                                 >  2:145172/1‑144 (MQ=255)
                                                                            aTTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTg                                                                >  4:359436/1‑148 (MQ=255)
                                                                                      gcgcTATTACCGCCAGCCATGGTGATATTGGCATCGCTGCTGTTGACGGGGAGTTTACGGTGAAAAAATTGCAAGTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCt                                                      <  4:158799/148‑1 (MQ=255)
                                                                                       cgcTATAACGGCCTGAAATGGTGATATTGTCATCGCTGCTGTTGACGGCTATTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTgg                                                    <  2:245809/149‑1 (MQ=255)
                                                                                               ccgtaatccaTGTTGATATTGTCATCGCTTCTGTTGACGGATAGTTTACGGTGAAAAAATTGTAACTAAGCCCGACGTTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCttt                                            <  6:197515/142‑1 (MQ=255)
                                                                                                       atttgtgtTATTGTATTCGCTGCTTTTTATTGGGTGTTTACGGTTAAAAATTTGCATCTACTCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTtggtgtggt                                    <  4:155908/145‑1 (MQ=255)
                                                                                                           gTGATATTGTCAGCGATGCTGTTGACGGCGAGTTTACGGTTAAAAAATTGAAAATCCGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATc                                <  4:105712/149‑1 (MQ=255)
                                                                                                            tGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATc                                >  6:275112/1‑148 (MQ=255)
                                                                                                                 ttGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCAcgtc                          >  5:64978/1‑149 (MQ=255)
                                                                                                                  tGTCATGGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCAcgtc                          <  7:110029/148‑1 (MQ=255)
                                                                                                                    tCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCAt                                                                        >  3:20583/1‑100 (MQ=255)
                                                                                                                    tCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCAt                                                                        <  4:20583/100‑1 (MQ=255)
                                                                                                                    tCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCAcgtcgt                        <  6:64978/148‑1 (MQ=255)
                                                                                                                    tCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGtt                       <  3:329265/149‑1 (MQ=255)
                                                                                                                    tCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGtt                       <  5:275112/149‑1 (MQ=255)
                                                                                                                      aTCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTa                      <  3:359436/148‑1 (MQ=255)
                                                                                                                        cGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAgg                   <  2:51587/149‑1 (MQ=255)
                                                                                                                             ctgTTGACGGCTAGTTTACGATGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGa                <  7:311801/147‑1 (MQ=255)
                                                                                                                             ctgTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATg              >  8:280173/1‑149 (MQ=255)
                                                                                                                             ctgTTGACGGCGAGTTTACGATCAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGa                <  7:305997/147‑1 (MQ=255)
                                                                                                                              tgTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATgc             >  5:160414/1‑149 (MQ=255)
                                                                                                                                ttGACGGCGAGTTTACGGTGAAAAATTTGCAACTCCGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATgcgc           <  1:370522/149‑1 (MQ=255)
                                                                                                                                    cGGCTAGTTTACGGTGAAAAAATTGCACGTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGAt       <  7:173772/149‑1 (MQ=255)
                                                                                                                                      gCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCTATGCGCTGATGt     >  5:261001/1‑149 (MQ=255)
                                                                                                                                       cGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGtt    >  8:204640/1‑149 (MQ=255)
                                                                                                                                       cGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGtt    <  4:243732/149‑1 (MQ=255)
                                                                                                                                       agAGTTTAGGGTTAAAACATTCCACCTACGCCCGACTGTACAGCTTATTCCCATGAACATCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTAGATGTCTTTGGTGTGGGGATCCACGTCGTTAAGGCGATGCGCTGATGtt    <  2:201681/148‑1 (MQ=255)
                                                                                                                                        gAGTTTACGGTGAAAAAATTGCAACTACCCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGttt   <  8:364828/149‑1 (MQ=255)
                                                                                                                                          gTTTACGGTTAAAAAATTCCAACTACGCCCGACTGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTg  <  6:261001/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATGGTGATATTGTCATCGCTGCTGTTGACGGCGAGTTTACGGTGAAAAAATTGCAACTACGCCCGACGGTACAGCTTATTCCCATGAACAGCGCGTACTCGCCCATTACCATCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTG  >  NC_000913/1230907‑1231192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑