Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,819,614 C→T 5.2% A64A (GCG→GCA chbA ← N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,6140CT 5.2% 249.0 / 2.2 76A64A (GCG→GCAchbAN,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base C (37/35);  new base T (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACTTCTTCCAGCTCT  >  NC_000913/1819467‑1819761
                                                                                                                                                   |                                                                                                                                                   
tttttCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCGGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATgcg                                                                                                                                                    >  4:376437/1‑149 (MQ=255)
      aTGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCttcat                                                                                                                                              >  8:413207/1‑149 (MQ=255)
       tGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCttcatt                                                                                                                                             <  4:96536/149‑1 (MQ=255)
          aGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTTGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCaa                                                                                                                                          <  1:158552/149‑1 (MQ=255)
          aGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCaa                                                                                                                                          >  4:113822/1‑149 (MQ=255)
           gCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAAt                                                                                                                                         <  7:130755/149‑1 (MQ=255)
                  taattCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGCCTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATcc                                                                                                                                  >  8:341822/1‑149 (MQ=255)
                   aattCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATcc                                                                                                                                  <  7:413207/148‑1 (MQ=255)
                       cAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATACCATCCg                                                                                                                                 <  1:242532/145‑1 (MQ=255)
                       cAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATACCATCCg                                                                                                                                 >  2:242532/1‑145 (MQ=255)
                            aTCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGAt                                                                                                                         <  4:212306/148‑1 (MQ=255)
                                 ttCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTTGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatca                                                                                                                   <  3:114097/149‑1 (MQ=255)
                                 ttCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatca                                                                                                                   >  7:40347/1‑149 (MQ=255)
                                    aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATg                                                                                                                 <  3:113822/148‑1 (MQ=255)
                                      gcgcAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCt                                                                                                              >  3:181069/1‑149 (MQ=255)
                                          aaGCATGGACGTCCTTACATGATCCTGAGCGTGGACCAGCACCAGACGCACTTGCATCTTACCTTCGCCCGCATCGCCTTCAATCCGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCCTCCGTGACTGATCCATCATGGCTTTtg                                                                                                          <  3:263439/149‑1 (MQ=255)
                                              aTGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCCATCAGTTTCGTCTGTACCAGATGCGCTTCAGTCAATTCCAACCGTGACTGATCCAACATGGCTTTtgctgc                                                                                                      >  2:274678/1‑149 (MQ=255)
                                                ggACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATgg                                                                                                                <  5:403785/137‑1 (MQ=255)
                                                ggACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATgg                                                                                                                >  6:403785/1‑137 (MQ=255)
                                                ggACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCgg                                                                                                    >  5:188500/1‑149 (MQ=255)
                                                       aTTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATc                                                                                             >  5:411256/1‑149 (MQ=255)
                                                           aaTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCaa                                                                                                                                          >  6:155977/1‑100 (MQ=255)
                                                           aaTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCaa                                                                                                                                          <  5:155977/100‑1 (MQ=255)
                                                           aaTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGccc                                                                                         >  5:100530/1‑149 (MQ=255)
                                                           aaTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGccc                                                                                         >  4:102890/1‑149 (MQ=255)
                                                           aaTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGcc                                                                                          <  8:272608/148‑1 (MQ=255)
                                                            aTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGa                                                                                                                          <  8:57647/115‑1 (MQ=255)
                                                            aTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGa                                                                                                                          >  7:57647/1‑115 (MQ=255)
                                                            aTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCt                                                                                        >  2:281200/1‑149 (MQ=255)
                                                                   tGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGc                                                                                 >  7:425832/1‑149 (MQ=255)
                                                                     aGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGc                                                                                 <  2:208998/147‑1 (MQ=255)
                                                                     aGCGTCGACCAGCACCAGACTCACTTTCAGCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGGCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTGCGCCt                                                                               <  6:322637/149‑1 (MQ=255)
                                                                      gCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTg                                                                              >  2:321347/1‑149 (MQ=255)
                                                                        gTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGtt                                                                            <  3:166948/149‑1 (MQ=255)
                                                                            aCCAGCACCAGACTCACTTTCATCTTACCTTCGCCGGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAg                                                                        <  3:376437/149‑1 (MQ=255)
                                                                              cAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCa                                                                         <  2:292761/146‑1 (MQ=255)
                                                                              cAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCa                                                                         >  1:292761/1‑146 (MQ=255)
                                                                                    cAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCa                                                                 >  1:34075/1‑148 (MQ=255)
                                                                                     aGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATa                                                               <  8:425832/149‑1 (MQ=255)
                                                                                       aCTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCg                                                                                  >  3:233452/1‑128 (MQ=255)
                                                                                       aCTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCg                                                                                  <  4:233452/128‑1 (MQ=255)
                                                                                           aCTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAAt                                                                                                                                         >  4:168441/1‑69 (MQ=255)
                                                                                           aCTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAAt                                                                                                                                         <  3:168441/69‑1 (MQ=255)
                                                                                           aCTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCa                                                          <  7:341822/148‑1 (MQ=255)
                                                                                           aCTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAg                                                         >  4:174239/1‑149 (MQ=255)
                                                                                           aCTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAg                                                         >  4:252048/1‑149 (MQ=255)
                                                                                               tCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTg                                                     >  5:310845/1‑149 (MQ=255)
                                                                                                 aTCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTgc                                                    <  3:102890/148‑1 (MQ=255)
                                                                                                 aTCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTgc                                                    <  8:40347/148‑1 (MQ=255)
                                                                                                   cTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTgcgcg                                                 >  8:350889/1‑149 (MQ=255)
                                                                                                        cTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGTGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAg                                                                        <  5:6778/121‑1 (MQ=255)
                                                                                                        cTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGTGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAg                                                                        >  6:6778/1‑121 (MQ=255)
                                                                                                         ttCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACg                                                             <  8:327946/131‑1 (MQ=255)
                                                                                                         ttCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACg                                                             >  7:327946/1‑131 (MQ=255)
                                                                                                            gCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCg                                         >  6:167148/1‑148 (MQ=255)
                                                                                                                   tCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCg                                         >  8:19087/1‑141 (MQ=255)
                                                                                                                   tCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCg                                         <  7:19087/141‑1 (MQ=255)
                                                                                                                   tCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTtgat                                 >  6:221972/1‑149 (MQ=255)
                                                                                                                   tCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTtgat                                 <  2:34075/149‑1 (MQ=255)
                                                                                                                      ccTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTgatgat                              <  6:100530/149‑1 (MQ=255)
                                                                                                                        ttCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCACTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTgatgat                              >  5:335123/1‑147 (MQ=255)
                                                                                                                          cAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCa                            <  4:181069/147‑1 (MQ=255)
                                                                                                                                 tttCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATca                   >  8:190812/1‑149 (MQ=255)
                                                                                                                                     gcatGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATcaccac               <  7:244272/146‑1 (MQ=255)
                                                                                                                                     gTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATcaccac               <  6:310352/149‑1 (MQ=255)
                                                                                                                                      tCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCa                                                                         <  1:65295/90‑1 (MQ=255)
                                                                                                                                      tCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCa                                                                         >  2:65295/1‑90 (MQ=255)
                                                                                                                                       cTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCActt             >  8:101692/1‑149 (MQ=255)
                                                                                                                                          tACCAGATGTGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGa                                       <  8:17445/120‑1 (MQ=255)
                                                                                                                                          tACCAGATGTGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGa                                       >  7:17445/1‑120 (MQ=255)
                                                                                                                                          tACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCActtctt          <  3:252048/149‑1 (MQ=255)
                                                                                                                                            ccAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACTTCTTcc        <  3:385498/149‑1 (MQ=255)
                                                                                                                                             cAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACttcttc         >  1:129637/1‑147 (MQ=255)
                                                                                                                                             cAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACttcttc         >  3:369928/1‑147 (MQ=255)
                                                                                                                                             cAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACttcttc         >  3:264131/1‑147 (MQ=255)
                                                                                                                                               gATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCGCCACTTCTTCCAGc     >  3:234087/1‑149 (MQ=255)
                                                                                                                                                 tGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACTTCTTCCAGctc   <  7:101692/149‑1 (MQ=255)
                                                                                                                                                  gcgcTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACTTCTTCCAGctct  >  5:32651/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGCCAGGCTGCGCGCTTGTCCGGAGTTGATGATCAGCCCCATCACCACTTCTTCCAGCTCT  >  NC_000913/1819467‑1819761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: