Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,353,935 G→A 5.9% R430H (CGT→CAT)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,353,9350GA 5.9% 214.7 / 2.4 67R430H (CGT→CAT) glpAanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base A (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

TGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGCCGC  >  NC_000913/2353789‑2354080
                                                                                                                                                  |                                                                                                                                                 
tGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCg                                                                                                                                                   >  4:325347/1‑147 (MQ=255)
  ccGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGc                                                                                                                                               >  2:416124/1‑149 (MQ=255)
    gATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCt                                                                                                                                             >  1:130588/1‑149 (MQ=255)
        tGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTa                                                                                                                                         >  7:128915/1‑149 (MQ=255)
           cACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTAtgt                                                                                                                                      <  5:330350/149‑1 (MQ=255)
           cACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTAtgt                                                                                                                                      <  7:313592/149‑1 (MQ=255)
            aCTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTAtgt                                                                                                                                      >  3:370067/1‑148 (MQ=255)
             cTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGa                                                                                                                                    <  8:71495/149‑1 (MQ=255)
              tGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAg                                                                                                                                   >  7:150973/1‑149 (MQ=255)
               gCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGa                                                                                                                                    <  7:30724/147‑1 (MQ=255)
               gCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGa                                                                                                                                    >  8:30724/1‑147 (MQ=255)
                ccTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTa                                                                                                                                                 <  7:46276/133‑1 (MQ=255)
                ccTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTa                                                                                                                                                 >  8:46276/1‑133 (MQ=255)
                    gTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGaa                                                                                                                             <  8:389697/149‑1 (MQ=255)
                     ttCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAg                                                                                                                            <  6:7913/149‑1 (MQ=255)
                         caAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGt                                                                                                                        <  3:18920/149‑1 (MQ=255)
                         caAGAACCCGCCGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCgg                                                                                                                         >  8:176172/1‑148 (MQ=255)
                          aaGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGt                                                                                                                        >  8:121473/1‑148 (MQ=255)
                               cccGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGc                                                                                                                  <  4:370067/149‑1 (MQ=255)
                               cccGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGc                                                                                                                  >  2:9509/1‑149 (MQ=255)
                                ccGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCg                                                                                                                 <  5:187136/149‑1 (MQ=255)
                                 cGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCCCCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCgg                                                                                                                >  4:141727/1‑149 (MQ=255)
                                 cGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCgg                                                                                                                >  2:54427/1‑149 (MQ=255)
                                 cGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCgg                                                                                                                <  8:128915/149‑1 (MQ=255)
                                 cGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCACGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCt                                                                                                                                             >  1:224514/1‑120 (MQ=255)
                                 cGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCACGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCt                                                                                                                                             <  2:224514/120‑1 (MQ=255)
                                         ttACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTg                                                                                                        <  7:176172/149‑1 (MQ=255)
                                         ttACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTg                                                                                                        <  2:130588/149‑1 (MQ=255)
                                         ttACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTg                                                                                                        <  1:416124/149‑1 (MQ=255)
                                         ttACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTg                                                                                                        >  2:372092/1‑149 (MQ=255)
                                                   tAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCgg                                                                                              <  8:125826/149‑1 (MQ=255)
                                                   tAAAGTCAACTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCgg                                                                                              >  2:36771/1‑149 (MQ=255)
                                                        tCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGa                                                                                          >  2:236082/1‑148 (MQ=255)
                                                             tcCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCg                                                                                                                          >  7:290496/1‑111 (MQ=255)
                                                             tcCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCg                                                                                                                          <  8:290496/111‑1 (MQ=255)
                                                               cctgcctgCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTa                                                                                   <  3:325347/148‑1 (MQ=255)
                                                               cctgcctgCCCCGCTGCGCGGTTCTGCGGTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTaaa                                                                                 <  4:200677/149‑1 (MQ=255)
                                                                  gcctgccCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACg                                                                               >  2:263663/1‑149 (MQ=255)
                                                                   cctgccCCGCTGCGCGGTTCTGCGGTTTTTCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGGATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGt                                                                              >  3:209514/1‑149 (MQ=255)
                                                                       ccccGCTGCGCGGTTCTGCGGTTTATCGTCATGTCGATCGCACGCCTGACTGGCTGAGCGAAGGCCGTATGCAACGTAGCCTGTTATGTTAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAAt                                                                          <  3:370948/149‑1 (MQ=255)
                                                                                gcgGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCctgctg                                                                 >  1:382705/1‑149 (MQ=255)
                                                                                   gTTCTGCGGTTTATCGTCATGGCGATCGCTCGCCTGCCTGGCTGAGCGTAGGCCGTCTGCACCGTAGCGTGGTATGTGAGTGGGAAGCGGTAAGTGCGGGTTAAGTTGAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGAt                                                              <  1:36771/149‑1 (MQ=255)
                                                                                      cTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGAttt                                                            <  7:47224/148‑1 (MQ=255)
                                                                                       tGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCATAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCctgct                                                                  <  4:240770/141‑1 (MQ=255)
                                                                                       tGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCATAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCctgct                                                                  >  3:240770/1‑141 (MQ=255)
                                                                                       tGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCATAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCctgct                                                                  <  2:344705/141‑1 (MQ=255)
                                                                                       tGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCATAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCctgct                                                                  >  1:344705/1‑141 (MQ=255)
                                                                                         cGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGc                                                        >  4:132891/1‑149 (MQ=255)
                                                                                           gTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCcg                                                      <  1:372092/149‑1 (MQ=255)
                                                                                                tCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTa                                                 >  4:286538/1‑149 (MQ=255)
                                                                                                  gTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTAcc                                               >  8:41826/1‑149 (MQ=255)
                                                                                                   tCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAAc                                                                                >  1:317753/1‑115 (MQ=255)
                                                                                                   tCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAAc                                                                                <  2:317753/115‑1 (MQ=255)
                                                                                                   tCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTAccc                                              >  6:303515/1‑149 (MQ=255)
                                                                                                     aTGGCGATCGCACGCCTGCCTGGCTGAGCGACGGCCGTCTGCACCGTAGCCTGGGATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCgt                                            >  2:354376/1‑149 (MQ=255)
                                                                                                              gCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATg                                   >  8:159736/1‑149 (MQ=255)
                                                                                                              gCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATg                                   <  5:303515/149‑1 (MQ=255)
                                                                                                                aCGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATggg                                 <  7:121473/149‑1 (MQ=255)
                                                                                                                  gcctgcctGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCa                               >  1:323581/1‑149 (MQ=255)
                                                                                                                          ggCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGATAATTTAAACGGTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAg                       >  3:377878/1‑149 (MQ=255)
                                                                                                                           gCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCa                        <  8:150973/147‑1 (MQ=255)
                                                                                                                           gCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAgg                      <  7:89038/149‑1 (MQ=255)
                                                                                                                           gCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAgg                      <  3:132891/149‑1 (MQ=255)
                                                                                                                                gCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGaa                 <  2:323581/149‑1 (MQ=255)
                                                                                                                                      gCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTgc           <  4:369200/149‑1 (MQ=255)
                                                                                                                                      gCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTgc           >  3:369200/1‑149 (MQ=255)
                                                                                                                                          tCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCt       >  6:268613/1‑149 (MQ=255)
                                                                                                                                               aCCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGCACTCTGCGCCTGCcgc  >  6:236583/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGCCGC  >  NC_000913/2353789‑2354080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: