Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,080,621 T→G 7.8% Q203P (CAA→CCA)  fdhE ← formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,6210TG 7.8% 275.2 / 12.2 90Q203P (CAA→CCA) fdhEformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base T (45/38);  new base G (4/3);  total (49/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTATC  >  NC_000913/4080475‑4080766
                                                                                                                                                  |                                                                                                                                                 
cTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGc                                                                                                                                                 <  7:132199/149‑1 (MQ=255)
 tttAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa                                                                                                                                                >  3:59699/1‑149 (MQ=255)
 tttAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCa                                                                                                                                                >  6:6570/1‑149 (MQ=255)
   tAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCAc                                                                                                                                               <  6:5088/148‑1 (MQ=255)
    aaTCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCAcc                                                                                                                                              <  3:294597/148‑1 (MQ=255)
      tCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATg                                                                                                                                           >  2:423524/1‑149 (MQ=255)
      tCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCact                                                                                                                                           >  2:37212/1‑147 (MQ=255)
      tCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCacg                                                                                                                                           >  6:48680/1‑147 (MQ=255)
         cgGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactcag                                                                                                                                        <  2:367371/149‑6 (MQ=255)
           gCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactcaggg                                                                                                                                      <  1:37212/149‑8 (MQ=255)
           gCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCAAATTGGCACCactcaggg                                                                                                                                      <  5:48680/149‑8 (MQ=255)
              tGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGacac                                                                                                                                   >  1:197323/1‑149 (MQ=255)
                      aTCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATa                                                                                                                            <  8:316785/148‑1 (MQ=255)
                       tCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGCCACCGGCATAGa                                                                                                                          >  8:408689/1‑149 (MQ=255)
                       tCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAg                                                                                                                           >  5:2173/1‑148 (MQ=255)
                          aGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGa                                                                                                                          <  2:32541/146‑1 (MQ=255)
                          aGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGa                                                                                                                          >  1:32541/1‑146 (MQ=255)
                          aGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAAc                                                                                                                        >  5:352336/1‑148 (MQ=255)
                              aCCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACcaca                                                                                                                    >  6:244387/1‑148 (MQ=255)
                                     aTGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa                                                                                                                         >  5:76384/1‑136 (MQ=255)
                                     aTGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGaa                                                                                                                         <  6:76384/136‑1 (MQ=255)
                                        cAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCaa                                                                                                          >  5:148678/1‑148 (MQ=255)
                                        cAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCaa                                                                                                          <  5:244387/148‑1 (MQ=255)
                                         aGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGAGCACTACGTTCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAata                                                                                                        <  5:261482/149‑1 (MQ=255)
                                           tttGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCCCCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATAtt                                                                                                      >  5:179533/1‑149 (MQ=255)
                                           tttGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAACCCGGGCAATAtt                                                                                                      >  8:170135/1‑149 (MQ=255)
                                           tttGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATAtt                                                                                                      >  2:183212/1‑149 (MQ=255)
                                                 gCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGtt                                                                                                >  3:74230/1‑149 (MQ=255)
                                                  ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAAcc                                                                                                                >  1:411536/1‑132 (MQ=255)
                                                  ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAAcc                                                                                                                <  2:411536/132‑1 (MQ=255)
                                                  ctctGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTg                                                                                               >  6:169721/1‑149 (MQ=255)
                                                     tGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTc                                                                                            >  6:400615/1‑149 (MQ=255)
                                                       cTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTc                                                                                            <  2:51084/147‑1 (MQ=255)
                                                        tCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGcc                                                                                         >  8:61753/1‑149 (MQ=255)
                                                          gCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGcc                                                                                         <  1:183212/147‑1 (MQ=255)
                                                          gCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGt                                                                                       <  1:423524/149‑1 (MQ=255)
                                                                  tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTa                                                                                      >  6:42304/1‑142 (MQ=255)
                                                                  tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTa                                                                                      <  5:42304/142‑1 (MQ=255)
                                                                  tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                               >  2:179008/1‑149 (MQ=255)
                                                                  tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAgcg                                                                               >  3:104962/1‑149 (MQ=255)
                                                                  tgcATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGCATTCAgcg                                                                               >  5:378420/1‑149 (MQ=255)
                                                                      ttttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTa                                                                                      >  3:74207/1‑138 (MQ=255)
                                                                      ttttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTa                                                                                      <  4:74207/138‑1 (MQ=255)
                                                                       tttACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACAATGCTGGACACCGGCATAGAACAACAACCCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGc                                                                          >  6:285657/1‑149 (MQ=255)
                                                                                aCTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg                                                                 <  1:179008/149‑1 (MQ=255)
                                                                                 cTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCggg                                                                 >  5:138472/1‑148 (MQ=255)
                                                                                  tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAata                                                                                                        <  4:289622/108‑1 (MQ=255)
                                                                                  tACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAata                                                                                                        >  3:289622/1‑108 (MQ=255)
                                                                                    cGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTg                                                                      >  3:120478/1‑140 (MQ=255)
                                                                                    cGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTg                                                                      <  4:120478/140‑1 (MQ=255)
                                                                                       gCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTAt                                                                                     <  1:191377/122‑1 (MQ=255)
                                                                                        ccATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGa                                                          <  3:174235/148‑1 (MQ=255)
                                                                                        ccATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGa                                                          <  4:104962/148‑1 (MQ=255)
                                                                                        ccATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGa                                                          <  5:318943/148‑1 (MQ=255)
                                                                                        ccATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAt                                                         <  5:6570/149‑1 (MQ=255)
                                                                                         cATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGAtt                                                        >  7:166199/1‑149 (MQ=255)
                                                                                           ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg                                                      >  6:83839/1‑149 (MQ=255)
                                                                                           ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg                                                      <  6:148678/149‑1 (MQ=255)
                                                                                           ttCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg                                                      >  7:274044/1‑149 (MQ=255)
                                                                                                  tCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTg                                               >  1:79571/1‑149 (MQ=255)
                                                                                                   cacacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgc                                              >  5:49075/1‑149 (MQ=255)
                                                                                                    acacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgcg                                             <  3:262490/149‑1 (MQ=255)
                                                                                                    acacaGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTgc                                              <  2:197323/148‑1 (MQ=255)
                                                                                                        aGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg                                                      >  5:393376/1‑136 (MQ=255)
                                                                                                        aGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTgg                                                      <  6:393376/136‑1 (MQ=255)
                                                                                                         ggTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg                                                       <  3:29675/134‑1 (MQ=255)
                                                                                                         ggTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTg                                                       >  4:29675/1‑134 (MQ=255)
                                                                                                           ttGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTa                                      <  5:400615/149‑1 (MQ=255)
                                                                                                              cagtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTagag                                   <  6:2173/149‑1 (MQ=255)
                                                                                                              cagtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGCATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTaga                                    <  6:378420/148‑1 (MQ=255)
                                                                                                                gtgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGc                                  >  2:317404/1‑148 (MQ=255)
                                                                                                                 tgcagGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGa                                >  8:56686/1‑149 (MQ=255)
                                                                                                                    agGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAg                             >  8:357802/1‑149 (MQ=255)
                                                                                                                      gTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTg                           >  4:62529/1‑149 (MQ=255)
                                                                                                                       tAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTg                           >  5:86722/1‑148 (MQ=255)
                                                                                                                         aCGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCa                         <  7:56686/148‑1 (MQ=255)
                                                                                                                         aCGTAGCCCCTGAGGGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCa                         <  5:367737/148‑1 (MQ=255)
                                                                                                                           gTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGAc                                                                                                   <  1:154248/72‑1 (MQ=255)
                                                                                                                           gTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGAc                                                                                                   >  2:154248/1‑72 (MQ=255)
                                                                                                                           gTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGcc                      >  6:124271/1‑149 (MQ=255)
                                                                                                                              gCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCa                    <  5:83839/148‑1 (MQ=255)
                                                                                                                                tccagcatggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGa                  >  2:273337/8‑148 (MQ=255)
                                                                                                                                tccagcatggtgCAAATTGGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGa                  >  1:16518/8‑148 (MQ=255)
                                                                                                                                cccTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGAt                 <  6:352336/149‑1 (MQ=255)
                                                                                                                                   tGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATaaa              >  5:333972/1‑149 (MQ=255)
                                                                                                                                     aGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAAACGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCACAGATAAACg            >  4:37729/1‑149 (MQ=255)
                                                                                                                                          tgCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATAATGACGTTGCTCGCCGTAATCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCAACTGCGCCCAGTAGAGCGACAGTGCACCCCAGATAAACGGCGCt       <  3:37729/149‑1 (MQ=255)
                                                                                                                                              aaTTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTAt   <  6:333972/149‑1 (MQ=255)
                                                                                                                                              aaTTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTAt   <  8:166199/149‑1 (MQ=255)
                                                                                                                                               aTTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTATc  >  3:342663/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTTAATCGCGGCCTGTTCGTCATCCAGCGACCAGTAATGCAGTTTGCCGCTCTGCTCGCAGTTGCTGCATTTTACGCGCACTACGTGCCATTCAGTTTCACACAGGTTGCAGTGCAGGTAACGTAGCCCCTGAGTGGTGCCAATTTGCACCATGCTGGACACCGGCATAGAACCACAAACCGGGCAATATTGACGTTGCTCGCCGTATTCAGCGCGGGCTTTGCCGGGGATCAGATTGGCCATCTGCGCCCAGTAGAGCGACAGTGCAGCCCAGATAAACGGCGCTTTATC  >  NC_000913/4080475‑4080766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: