Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,234,855 C→T 5.4% N366N (AAC→AAT pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,234,8550CT 5.4% 293.5 / 4.3 93N366N (AAC→AATpgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (43/44);  new base T (2/3);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

GCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGAT  >  NC_000913/4234711‑4235002
                                                                                                                                                |                                                                                                                                                   
gcTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtg                                                                                                                                                 <  5:413347/149‑1 (MQ=255)
 cTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtgt                                                                                                                                                >  1:209895/1‑149 (MQ=255)
     ttGGCATCTGGTACAACAATTTCTTTGGTGCGGATACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCATGGCTATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGAt                                                                                                                                            <  8:285706/149‑1 (MQ=255)
        gCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAATGTTGTGGATTa                                                                                                                                          <  2:194219/148‑1 (MQ=255)
        gCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTAc                                                                                                                                         >  1:115569/1‑149 (MQ=255)
        gCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTAc                                                                                                                                         >  4:350333/1‑149 (MQ=255)
        gCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTAc                                                                                                                                         <  4:270607/149‑1 (MQ=255)
                 acaacaATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGc                                                                                                                                >  8:367488/1‑149 (MQ=255)
                 acaacaATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGc                                                                                                                                >  8:264595/1‑149 (MQ=255)
                  caacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGcc                                                                                                                               <  5:389181/149‑1 (MQ=255)
                   aacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTTGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGccc                                                                                                                              >  6:159468/1‑149 (MQ=255)
                   aacaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGccc                                                                                                                              <  7:162891/149‑1 (MQ=255)
                    acaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGccc                                                                                                                              >  3:243529/1‑148 (MQ=255)
                     caaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGa                                                                                                                            <  6:248838/149‑1 (MQ=255)
                           cTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtgt                                                                                                                                                <  4:252377/123‑1 (MQ=255)
                            tttGGTGAGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACAGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                                                                                                                     >  1:260289/1‑149 (MQ=255)
                              tGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                                                                                                                     <  7:264595/147‑1 (MQ=255)
                              tGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                                                                                                                     <  7:367488/147‑1 (MQ=255)
                              tGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTg                                                                                                                     <  6:180513/147‑1 (MQ=255)
                                   cGGAAACTGCAGCGATTCTGCCGTATGACCAGTATATGCACCGTGTCGCGGCGTACTTCCAGCAGGGCAATCTGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGaa                                                                                                              <  2:292896/149‑1 (MQ=255)
                                   cGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTgggg                                                                                                                  <  8:27631/145‑1 (MQ=255)
                                   cGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACCGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTgggg                                                                                                                  >  7:27631/1‑145 (MQ=255)
                                        aCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAgg                                                                                                         >  8:420461/1‑149 (MQ=255)
                                          tGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCa                                                                                                       >  5:121391/1‑149 (MQ=255)
                                           gAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCAc                                                                                                      >  6:188990/1‑149 (MQ=255)
                                            aaGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCa                                                                                                       <  4:203351/147‑1 (MQ=255)
                                              gCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAATGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTaa                                                                                                   >  8:141321/1‑149 (MQ=255)
                                              gCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAATGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTaa                                                                                                   >  4:14511/1‑149 (MQ=255)
                                                  ttCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGt                                                                                               <  5:188990/149‑1 (MQ=255)
                                                  ttCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGAATATCTGGGGTGAACCAGGCGCTAAAGGt                                                                                               >  6:89422/1‑149 (MQ=255)
                                                    cTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                                              <  3:350333/148‑1 (MQ=255)
                                                    cTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTc                                                                                              <  1:187761/148‑1 (MQ=255)
                                                    cTGCCGGATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTCGGGTGAACCAGGCACTAACGGTc                                                                                              <  8:117119/148‑1 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAATGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGc                                                                                           <  7:141321/148‑1 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAATGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGc                                                                                           <  3:14511/148‑1 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTgggg                                                                                                                  <  6:378311/125‑1 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTgggg                                                                                                                  >  5:378311/1‑125 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAgg                                                                                                         >  5:84536/1‑134 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAgg                                                                                                         <  6:84536/134‑1 (MQ=255)
                                                       ccGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGGGAACCAGGCACTAACGGTCa                                                                                             <  8:409780/146‑1 (MQ=255)
                                                        cGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGTAGTAAAACGGTAAGTATGTTGACCGTAAAGGTAACCTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACTGTCAGAAc                                                                                         >  7:416119/1‑149 (MQ=255)
                                                          tATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAcgc                                                                                       <  4:243529/149‑1 (MQ=255)
                                                               ccAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCt                                                                                  <  2:115569/149‑1 (MQ=255)
                                                                 aGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTAc                                                                                <  6:121391/149‑1 (MQ=255)
                                                                   tataTGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCa                                                                                                           <  6:285682/120‑1 (MQ=255)
                                                                     tatGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCa                                                                              <  2:396435/147‑1 (MQ=255)
                                                                        gCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGATAAGTATGTTGACCGTAACGGTAACCTTGTGGATTACCAGACTGGCCCGCTTATCTGGGGTGCACCAGCCACTAACGGTCAGCACGCGTTCGACCAGCTGa                                                                         >  6:338603/1‑149 (MQ=255)
                                                                         cACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTggg                                                                                                                   <  6:414226/106‑1 (MQ=255)
                                                                         cACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTggg                                                                                                                   >  5:414226/1‑106 (MQ=255)
                                                                         cACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGAt                                                                        >  5:218289/1‑149 (MQ=255)
                                                                         cACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGAt                                                                        >  1:404122/1‑149 (MQ=255)
                                                                          cccGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGCCTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATc                                                                       <  7:392522/148‑1 (MQ=255)
                                                                          aCCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAAAGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGCACCAGGCACTAACGGTCAGCACGCGTTATACCAGCAGAta                                                                       >  4:35498/1‑148 (MQ=255)
                                                                                tCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATccacca                                                                  <  2:260289/148‑1 (MQ=255)
                                                                                 cgcgGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAg                                                                 <  7:420461/148‑1 (MQ=255)
                                                                                    ggcgTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACAGTAACGGTAACGGTGTGTATTTCCAGCCTGGCCCGATTATCTGGGGTGCACCAGGCCCTAACGGTCCGCACGCGTTCTACCAGCTGATCCACCAGGGaa                                                             >  7:291890/1‑149 (MQ=255)
                                                                                          cTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCaaaa                                                       >  1:328437/1‑149 (MQ=255)
                                                                                          cTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCaaaa                                                       >  4:205833/1‑149 (MQ=255)
                                                                                          cTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCaaaa                                                       <  1:65976/149‑1 (MQ=255)
                                                                                           ttCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGCCTGGCCCGATTATCTGGGGAGAACCAGGCACTAACGTTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                                      >  6:83010/1‑149 (MQ=255)
                                                                                           ttCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                                      >  8:326697/1‑149 (MQ=255)
                                                                                           ttCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                                      >  1:326573/1‑149 (MQ=255)
                                                                                           ttCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                                      >  3:328380/1‑149 (MQ=255)
                                                                                                 cagGGCAATATGGAGGCCAACGGTAAGTATGGTGACCGTACCGGTAACGTTGTGGATTACCAGACTGGCCCGACTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTAcc                                                <  5:89422/149‑1 (MQ=255)
                                                                                                           tGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCCCGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGAttt                                       >  6:308445/1‑148 (MQ=255)
                                                                                                            ggAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGAc                                                                                                                                    >  5:353021/1‑54 (MQ=255)
                                                                                                            ggAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGAc                                                                                                                                    <  6:353021/54‑1 (MQ=255)
                                                                                                             gAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCATACTGGCACGATTATCTGGGGTGAACAATGAACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCAt                                    <  1:121172/149‑1 (MQ=255)
                                                                                                             gAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGCCTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCAt                                    <  7:199026/149‑1 (MQ=255)
                                                                                                              aGTCCAACGGTAAGTATGTTGACCGTAACGGTAAAGTTTTGTATTACCATACTTGCACGATTATCTGGGTTTAACCAGGAACTAACGGTCAGAACTCGTTCTAACAGCTGATACACCAGGGAACCAAAATGGTACCGTGCGATTTCATc                                   >  3:246598/1‑149 (MQ=255)
                                                                                                               gTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGAt                                         >  6:106194/1‑142 (MQ=255)
                                                                                                               gTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGAt                                         <  5:106194/142‑1 (MQ=255)
                                                                                                                tCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGc                                 >  4:174893/1‑149 (MQ=255)
                                                                                                                  cAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                                      <  1:216156/126‑1 (MQ=255)
                                                                                                                  cAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAAt                                                      >  2:216156/1‑126 (MQ=255)
                                                                                                                  cAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTc                               <  6:218289/149‑1 (MQ=255)
                                                                                                                  cAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTc                               >  5:260273/1‑149 (MQ=255)
                                                                                                                  cAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTc                               >  7:1159/1‑149 (MQ=255)
                                                                                                                    aCGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGa                                                              >  7:179815/1‑116 (MQ=255)
                                                                                                                    aCGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGa                                                              <  8:179815/116‑1 (MQ=255)
                                                                                                                     cGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGGTCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCgg                            >  1:216002/1‑149 (MQ=255)
                                                                                                                     cGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                                            <  7:61406/117‑1 (MQ=255)
                                                                                                                     cGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAAc                                                            >  8:61406/1‑117 (MQ=255)
                                                                                                                     cGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCgg                            <  2:209895/149‑1 (MQ=255)
                                                                                                                      ggTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTAc                                                                                >  4:229336/1‑96 (MQ=255)
                                                                                                                          aGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt                        >  2:399115/1‑148 (MQ=255)
                                                                                                                           gTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGTGTGAACCAGGCACTAACTGTCAGCACGCGGTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCa                      <  4:246598/149‑1 (MQ=255)
                                                                                                                                ttGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt                        >  5:302085/1‑142 (MQ=255)
                                                                                                                                ttGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt                        <  6:302085/142‑1 (MQ=255)
                                                                                                                                 tGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAcg                                                                                        >  2:95910/1‑77 (MQ=255)
                                                                                                                                 tGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCAcg                                                                                        <  1:95910/77‑1 (MQ=255)
                                                                                                                                   aCCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAg                                                                 >  7:401135/1‑98 (MQ=255)
                                                                                                                                   aCCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAg                                                                 <  8:401135/98‑1 (MQ=255)
                                                                                                                                           ggtaacgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGctcgc      >  3:3984/1‑147 (MQ=255)
                                                                                                                                               acgTTGTGGATGACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGGTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGat  <  8:376277/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGAT  >  NC_000913/4234711‑4235002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: