| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,095,453 | 0 | C | G | 20.8% | 55.5 / 10.1 | 24 | intergenic (‑16/‑52) | insE1/ymdE | IS3 transposase A/pseudogene |
| Reads supporting (aligned to +/- strand): ref base C (11/8); new base G (3/2); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGA > NC_000913/1095387‑1095598 | aGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCg > 7:72835/1‑149 (MQ=255) aaTGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAg < 8:72835/149‑1 (MQ=255) cTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCaa < 5:26352/149‑1 (MQ=255) tGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAAt > 1:81677/1‑149 (MQ=255) ttttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGa > 3:31396/1‑116 (MQ=255) ttttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGa < 4:31396/116‑1 (MQ=255) gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGcac < 8:50635/96‑1 (MQ=255) gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGcac > 7:50635/1‑96 (MQ=255) gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt > 2:108261/1‑149 (MQ=255) ttGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGc < 3:116611/88‑1 (MQ=33) ttGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGc > 4:116611/1‑88 (MQ=33) aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGAtt < 5:101397/130‑1 (MQ=255) aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGAtt > 6:101397/1‑130 (MQ=255) gTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGc > 7:117097/1‑149 (MQ=255) tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAg > 2:197539/1‑113 (MQ=255) tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAg < 1:197539/113‑1 (MQ=255) ttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTc < 8:117097/148‑1 (MQ=255) ggctaagtgAGTAAACTCTCAGTCAGAGGTGACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGc > 6:39942/7‑149 (MQ=37) ggctaagtgAGTAAACTCTCAGTCAGAGGTGACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGc < 5:39942/143‑1 (MQ=37) ctaagtgAGTAAACTCTCAGTCAGAGGTGACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCt > 6:151670/5‑124 (MQ=25) ctaagtgAGTAAACTCTCAGTCAGAGGTGACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCt < 5:151670/120‑1 (MQ=25) agtgAGTAAACTCTCAGTCAGAGGTGACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCagag > 5:43783/2‑149 (MQ=38) aCCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGt > 2:198517/1‑149 (MQ=255) aCCTCTGACTGAGAGTTTACTCACTTAGACGCGAGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATGATCTTGCCGGACTGGATATCGCTTCGCCAGAGAATCAGGt > 7:53452/1‑149 (MQ=255) actgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCtga < 1:108261/147‑1 (MQ=255) | AGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGA > NC_000913/1095387‑1095598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |