Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,340	C→T	intergenic (‑257/‑30)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,340	0	C	T	100.0%	147.3 / NA	42	intergenic (‑257/‑30)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/22);  total (20/22)

TGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC  >  NC_000913/1806205‑1806480                                                                                                                                        |                                                                                                                                             tGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAg                                                                                                                                 <  7:48621/149‑1 (MQ=255)    aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGa                                                                                                                                           >  6:64130/1‑136 (MQ=255)    aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGa                                                                                                                                           <  5:64130/136‑1 (MQ=255)       cTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCta                                                                                                                             >  1:115700/1‑147 (MQ=255)           tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATaggag                                                                                                                       >  2:176138/1‑149 (MQ=255)                   tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                                               >  1:193950/1‑149 (MQ=255)                   tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                                                <  2:202537/148‑1 (MQ=255)                     tGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCGTATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                                              <  2:182130/148‑1 (MQ=255)                       aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                            <  6:14852/148‑1 (MQ=255)                       aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                                           <  6:50716/149‑1 (MQ=255)                        aTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                                          <  2:115700/149‑1 (MQ=255)                            gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                                      >  2:145382/1‑149 (MQ=255)                              aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATag                                                                                                                          <  8:170083/127‑1 (MQ=255)                              aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATag                                                                                                                          >  7:170083/1‑127 (MQ=255)                              aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATc                                                                                                     >  8:110736/1‑148 (MQ=255)                              aTGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCATATAGGAGGAAATGATGGTACGTATc                                                                                                     >  6:118215/1‑148 (MQ=255)                                      aaaaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTg                                                                                            <  7:110736/149‑1 (MQ=255)                                                      tatatataTTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                                            >  2:51435/1‑149 (MQ=255)                                                        tatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                                          <  2:193950/149‑1 (MQ=255)                                                            tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGAt                                                                       <  5:118215/148‑1 (MQ=255)                                                              tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGATCGACTGAACTCAGCCTAGAGGATTAAATGATGGTACGTATCTCTACTATGACCCTTGAGCACTCTCTCGATAgc                                                                    >  4:123654/1‑149 (MQ=255)                                                                      cAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                                >  4:150213/1‑81 (MQ=255)                                                                        aaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                          <  1:176138/149‑1 (MQ=255)                                                                           ttttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAccc                                                       <  1:51435/149‑1 (MQ=255)                                                                              taaataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                                      <  4:140209/131‑1 (MQ=255)                                                                              taaataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                                      >  3:140209/1‑131 (MQ=255)                                                                                aataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCa                                                   >  5:119999/1‑148 (MQ=255)                                                                                   aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                             >  4:14920/1‑103 (MQ=255)                                                                                   aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                             <  3:14920/103‑1 (MQ=255)                                                                                     aGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                            >  4:152020/1‑86 (MQ=255)                                                                                     aGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                            <  3:152020/86‑1 (MQ=255)                                                                                     aGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTa                                             >  5:8912/1‑149 (MQ=255)                                                                                      gCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                                            <  3:123654/149‑1 (MQ=255)                                                                                          cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATcc                                          <  6:119999/147‑1 (MQ=255)                                                                                                         ttAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                                      >  1:68986/1‑72 (MQ=255)                                                                                                         ttAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                                                      <  2:68986/72‑1 (MQ=255)                                                                                                                     cTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCgg             >  5:5641/1‑149 (MQ=255)                                                                                                                      tttCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                    <  1:124844/77‑1 (MQ=255)                                                                                                                      tttCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                    >  2:124844/1‑77 (MQ=255)                                                                                                                           gCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                          <  7:97651/98‑1 (MQ=255)                                                                                                                           gCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                          >  8:97651/1‑98 (MQ=255)                                                                                                                                ttCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAAccc  <  6:8912/149‑1 (MQ=255)                                                                                                                                        |                                                                                                                                             TGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC  >  NC_000913/1806205‑1806480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑