mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L001_R1_001.good.fq | 229,781 | 29,475,874 | 100.0% | 128.3 bases | 149 bases | 95.5% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L001_R2_001.good.fq | 229,781 | 29,189,991 | 100.0% | 127.0 bases | 149 bases | 97.9% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L002_R1_001.good.fq | 186,356 | 23,835,426 | 100.0% | 127.9 bases | 149 bases | 95.2% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L002_R2_001.good.fq | 186,356 | 23,601,766 | 100.0% | 126.6 bases | 149 bases | 97.5% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L003_R1_001.good.fq | 211,466 | 27,051,402 | 100.0% | 127.9 bases | 149 bases | 95.4% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L003_R2_001.good.fq | 211,466 | 26,778,520 | 100.0% | 126.6 bases | 149 bases | 97.8% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L004_R1_001.good.fq | 183,491 | 23,441,868 | 100.0% | 127.8 bases | 149 bases | 95.1% |
| errors | Plate-2-BOP-27-PFKA-ALE-2-flask-11_S67_L004_R2_001.good.fq | 183,491 | 23,205,449 | 100.0% | 126.5 bases | 149 bases | 97.4% |
| total | 1,622,188 | 206,580,296 | 100.0% | 127.3 bases | 149 bases | 96.5% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 43.1 | 3.5 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 20299 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 184 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.012 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.85392 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 14:29:17 11 Sep 2020 | 14:29:41 11 Sep 2020 | 24 seconds |
| Read alignment to reference genome | 14:29:41 11 Sep 2020 | 14:32:34 11 Sep 2020 | 2 minutes 53 seconds |
| Preprocessing alignments for candidate junction identification | 14:32:34 11 Sep 2020 | 14:32:59 11 Sep 2020 | 25 seconds |
| Preliminary analysis of coverage distribution | 14:32:59 11 Sep 2020 | 14:33:41 11 Sep 2020 | 42 seconds |
| Identifying junction candidates | 14:33:41 11 Sep 2020 | 14:34:21 11 Sep 2020 | 40 seconds |
| Re-alignment to junction candidates | 14:34:21 11 Sep 2020 | 14:35:16 11 Sep 2020 | 55 seconds |
| Resolving best read alignments | 14:35:16 11 Sep 2020 | 14:35:53 11 Sep 2020 | 37 seconds |
| Creating BAM files | 14:35:53 11 Sep 2020 | 14:36:31 11 Sep 2020 | 38 seconds |
| Tabulating error counts | 14:36:31 11 Sep 2020 | 14:36:47 11 Sep 2020 | 16 seconds |
| Re-calibrating base error rates | 14:36:47 11 Sep 2020 | 14:36:49 11 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 14:36:49 11 Sep 2020 | 14:40:01 11 Sep 2020 | 3 minutes 12 seconds |
| Polymorphism statistics | 14:40:01 11 Sep 2020 | 14:40:02 11 Sep 2020 | 1 second |
| Output | 14:40:02 11 Sep 2020 | 14:40:17 11 Sep 2020 | 15 seconds |
| Total | 11 minutes 0 seconds | ||
