Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,340	C→T	intergenic (‑257/‑30)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,340	0	C	T	100.0%	116.5 / NA	34	intergenic (‑257/‑30)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

GCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGG  >  NC_000913/1806201‑1806483                                                                                                                                            |                                                                                                                                                gCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGaa                                                                                                                                         <  7:103919/148‑1 (MQ=255)      gTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                                   >  4:54372/1‑149 (MQ=255)       tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGcc                                                                                                                                  >  2:140195/1‑149 (MQ=255)       tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                                   <  3:54372/148‑1 (MQ=255)           cTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCtat                                                                                                                               <  1:140195/148‑1 (MQ=255)               tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATaggag                                                                                                                          >  3:109334/1‑149 (MQ=255)                     tttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAAt                                                                                                                                       <  4:87724/130‑1 (MQ=255)                          gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                                <  2:22288/148‑1 (MQ=255)                           aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                                               <  4:109334/148‑1 (MQ=255)                                        cGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                                   >  1:157026/1‑115 (MQ=255)                                        cGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                                   <  2:157026/115‑1 (MQ=255)                                               aGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCTCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACt                                                                                          >  6:12526/1‑149 (MQ=255)                                                        tttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctc                                                                                 >  3:153466/1‑149 (MQ=255)                                                          tatatataTTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                       <  6:40031/141‑1 (MQ=255)                                                          tatatataTTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                                       >  5:40031/1‑141 (MQ=255)                                                                         gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                                                  <  5:13605/99‑1 (MQ=255)                                                                         gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                                                  >  6:13605/1‑99 (MQ=255)                                                                         gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                                              >  2:46335/1‑135 (MQ=255)                                                                         gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                                              <  1:46335/135‑1 (MQ=255)                                                                              aTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACt                                                                                                                                            >  7:131685/1‑68 (MQ=255)                                                                              aTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACt                                                                                                                                            <  8:131685/68‑1 (MQ=255)                                                                                     ataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCaaa                                                    <  4:153466/149‑1 (MQ=255)                                                                                      taaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                   <  5:130141/149‑1 (MQ=255)                                                                                         aGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                   >  7:18148/1‑146 (MQ=255)                                                                                         aGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                   <  8:18148/146‑1 (MQ=255)                                                                                          gCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                                               >  6:70125/1‑149 (MQ=255)                                                                                            tCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                   <  4:117079/143‑1 (MQ=255)                                                                                            tCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                   >  3:117079/1‑143 (MQ=255)                                                                                              cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgc                                                                       >  4:68983/1‑121 (MQ=255)                                                                                              cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAgc                                                                       <  3:68983/121‑1 (MQ=255)                                                                                                                        aCTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCg                 >  1:122225/1‑149 (MQ=255)                                                                                                                                       ggTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGTAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCggg  <  5:12526/149‑1 (MQ=255)                                                                                                                                       ggTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGa                                      >  5:140881/1‑113 (MQ=255)                                                                                                                                       ggTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGa                                      <  6:140881/113‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                GCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGG  >  NC_000913/1806201‑1806483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑