Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,299,323:1 +A 5.4% coding (350/519 nt) yhbO → stress‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,299,3231.A 5.4% 291.4 / 12.4 74coding (350/519 nt)yhbOstress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base . (40/30);  new base A (2/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

GCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAG  >  NC_000913/3299175‑3299468
                                                                                                                                                     |                                                                                                                                                 
gCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCCCCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAGGTGATTCg                                                                                                                                                    >  7:148798/1‑149 (MQ=255)
 cccTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCgg                                                                                                                                                  <  8:217518/149‑1 (MQ=255)
   ctgctgCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCgggg                                                                                                                                                <  6:215184/149‑1 (MQ=255)
   ctgctgCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACACGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAAGTGAATCgggg                                                                                                                                                >  6:83008/1‑149 (MQ=255)
            ccGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCaaa                                                                                                                                         <  1:32165/147‑1 (MQ=255)
               ggcggcCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                                                                    <  1:134342/149‑1 (MQ=255)
                      aTTCACCGGATTATCTGCGTGGGGACAACCGGTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAACCTGACCGCAGt                                                                                                                              >  6:302321/1‑148 (MQ=255)
                          aCCGGATTATCTGCGTGGGGACCACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCCCGGCCCGCAGGTGCTGATCAGCGCCGATGGGATTCGGGGGCGCAACCTGCCCGCAGTTacac                                                                                                                         >  6:242196/1‑149 (MQ=255)
                            cGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAAcc                                                                                                                        >  2:191787/1‑148 (MQ=255)
                               aTTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATc                                                                                                                    <  3:248956/149‑1 (MQ=255)
                                   tCTGCTTGGGGACAACCGTTTTGTCACCTTTACCAGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCatta                                                                                                                >  3:289512/1‑149 (MQ=255)
                                   tCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTCTGAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCatt                                                                                                                 <  4:278838/148‑1 (MQ=255)
                                    cTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCattat                                                                                                               >  5:158427/1‑149 (MQ=255)
                                       cGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGa                                                                                                            <  3:132944/149‑1 (MQ=255)
                                        gTGGGGACAACCGTTTTGTCACCTTTACCGGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGAt                                                                                                           >  7:215178/1‑149 (MQ=255)
                                        gTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCTGTGTTTGCCATCTGTCACGGGCCGGAGTTCCTGATCAGCTCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGAt                                                                                                           <  5:83008/149‑1 (MQ=255)
                                        gTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGAt                                                                                                           <  3:93717/149‑1 (MQ=255)
                                        gTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGAt                                                                                                           <  6:37494/149‑1 (MQ=255)
                                        gTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATg                                                                                                          >  2:322072/1‑149 (MQ=255)
                                                 aCCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAAt                                                                                                  <  6:158427/149‑1 (MQ=255)
                                                   cGTTTTGTCACCTTTACACGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGc                                                                                                >  4:193719/1‑149 (MQ=255)
                                                    gTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGAGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCg                                                                                               <  8:119900/149‑1 (MQ=255)
                                                     ttttGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCgg                                                                                              <  2:60579/149‑1 (MQ=255)
                                                          tCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCgg                                                                                              <  5:63289/144‑1 (MQ=255)
                                                          tCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCgg                                                                                              >  6:63289/1‑144 (MQ=255)
                                                          tCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGcgcg                                                                                         >  6:191953/1‑149 (MQ=255)
                                                            aCCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCat                                                                                                                  <  8:105385/122‑1 (MQ=255)
                                                            aCCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCat                                                                                                                  >  7:105385/1‑122 (MQ=255)
                                                            aCCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCgg                                                                                        >  4:225127/1‑148 (MQ=255)
                                                                 tACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAAttt                                                                                   <  5:191953/148‑1 (MQ=255)
                                                                  aCCCGTTATTTTGTGAATAGTGGCCAACCGGTGTGTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAAtttt                                                                                  <  8:148798/148‑1 (MQ=255)
                                                                  aCCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTa                                                                                 <  1:322072/148‑1 (MQ=255)
                                                                   cccGTGATTTTGTGAATAGTTGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTa                                                                                 <  4:289512/148‑1 (MQ=255)
                                                                   cccGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTAc                                                                                <  4:178911/149‑1 (MQ=255)
                                                                    ccGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTAc                                                                                >  5:137519/1‑148 (MQ=255)
                                                                      gTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGAGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGa                                                                              >  7:162414/1‑149 (MQ=255)
                                                                      gTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGAGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGa                                                                              >  7:157803/1‑149 (MQ=255)
                                                                      gTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCa                                                                                                                                >  2:115512/1‑98 (MQ=255)
                                                                      gTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCa                                                                                                                                <  1:115512/98‑1 (MQ=255)
                                                                         aTTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAg                                                                          <  1:191787/149‑1 (MQ=255)
                                                                          ttttGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGAGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAg                                                                          <  8:157803/149‑1 (MQ=255)
                                                                          ttttGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGAGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAg                                                                          <  8:162414/149‑1 (MQ=255)
                                                                          ttttGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGCTGTTAAAAATGCGGGCGCGGAATTTTACGATCAg                                                                          >  6:14220/1‑148 (MQ=255)
                                                                           tttGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAgg                                                                         >  2:193733/1‑148 (MQ=255)
                                                                                   tAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTgg                                                                <  1:337815/149‑1 (MQ=255)
                                                                                   tAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATAAGGAAGTCGTgg                                                                >  7:136056/1‑149 (MQ=255)
                                                                                        gCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGCCCGCAGTTAACCCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGa                                                            >  6:48330/1‑148 (MQ=255)
                                                                                        gCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGa                                                            >  6:227171/1‑148 (MQ=255)
                                                                                        gCAAACCGGTGTTTGCCATCGGTCACGGCCCGAAGTTGCTGATCAGCGCCGATGTGATTCTGGGGCGCAAACTGACCGCAGTTAAAACGATCATTATTTATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGa                                                            >  6:227173/1‑148 (MQ=255)
                                                                                          aaaCCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATa                                                          >  6:306051/1‑148 (MQ=255)
                                                                                           aaCCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATa                                                          >  1:19929/1‑147 (MQ=255)
                                                                                              cGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGGTGATAAAGAt                                                     <  5:258690/149‑1 (MQ=255)
                                                                                               ggTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATc                                                    <  1:17973/149‑1 (MQ=255)
                                                                                                     tGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTgg                                              >  5:304063/1‑149 (MQ=255)
                                                                                                        cATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTa                                           <  6:304063/149‑1 (MQ=255)
                                                                                                        cATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTa                                           >  4:137519/1‑149 (MQ=255)
                                                                                                        cATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTa                                           >  7:221254/1‑149 (MQ=255)
                                                                                                         aTCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACTATCAGTAAGTCGTGGt                                                               >  8:288484/1‑128 (MQ=255)
                                                                                                         aTCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTAc                                          >  1:177372/1‑149 (MQ=255)
                                                                                                             gTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCa                                                   <  1:118099/136‑1 (MQ=255)
                                                                                                             gTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCa                                                   >  2:118099/1‑136 (MQ=255)
                                                                                                                aCGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCCGCTGGTTACAAGCCg                                    >  2:266766/1‑148 (MQ=255)
                                                                                                                aCGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCg                                    >  3:61393/1‑148 (MQ=255)
                                                                                                                aCGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCg                                    >  3:276311/1‑148 (MQ=255)
                                                                                                                     ccGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACAcc                              >  7:288878/1‑149 (MQ=255)
                                                                                                                          gTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGAc                          >  4:153053/1‑148 (MQ=255)
                                                                                                                             gCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGAt                       <  1:193733/148‑1 (MQ=255)
                                                                                                                             gCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATc                      >  2:36020/1‑149 (MQ=255)
                                                                                                                                         cGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTa          <  3:153053/149‑1 (MQ=255)
                                                                                                                                             gtgATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGTTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACcg      >  4:863/1‑149 (MQ=255)
                                                                                                                                             gtgATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGACGGACACCGGACGATCTGCCAGCGTTTAACcg      >  8:215744/1‑149 (MQ=255)
                                                                                                                                              tgATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGAt                                                     >  2:64761/1‑101 (MQ=255)
                                                                                                                                              tgATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGAt                                                     <  1:64761/101‑1 (MQ=255)
                                                                                                                                                aTTCGGGGGCGCAAACTGACCGCATTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGTAATTTTACGATCAGGAAGGCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGa   <  3:193719/149‑1 (MQ=255)
                                                                                                                                                aTTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACcgc     >  7:70317/1‑147 (MQ=255)
                                                                                                                                                aTTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACcgc     >  7:215098/1‑147 (MQ=255)
                                                                                                                                                aTTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGa   <  2:19929/149‑1 (MQ=255)
                                                                                                                                                 ttCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAg  >  8:145958/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAG  >  NC_000913/3299175‑3299468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: