Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,799,635 T→A 7.0% intergenic (+22/+15) alaE → / ← ygaC alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,799,6350TA 7.0% 246.1 / 3.1 79intergenic (+22/+15)alaE/ygaCalanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base A (2/4);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAG  >  NC_000913/2799487‑2799783
                                                                                                                                                    |                                                                                                                                                    
tCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCAt                                                                                                                                                      >  4:288061/1‑149 (MQ=255)
tCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCAt                                                                                                                                                      >  3:235275/1‑149 (MQ=255)
tCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGAATGGCAt                                                                                                                                                      >  3:239429/1‑149 (MQ=255)
  aGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCg                                                                                                                                                    <  7:12683‑M2/149‑2 (MQ=255)
      tATGGCGGCGGGCCGTTCAAACATCGTGGTTTCGATGTTGATGGGGGCGGGTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTGTAAAGTCTGCCGTTACCAGCAGGTAAAAGCCTGACTCTTATTTTCGCGACTGGCATCgcca                                                                                                                                                 >  2:240326‑M2/1‑144 (MQ=255)
        tGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCgccagtc                                                                                                                                              >  4:145084‑M2/1‑142 (MQ=255)
                gTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCttt                                                                                                                                      <  1:211512/149‑1 (MQ=255)
                   aGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCTGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTaa                                                                                                                                    <  8:265235/148‑1 (MQ=255)
                       cAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGCCTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGCCTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGc                                                                                                                               >  8:164600/1‑149 (MQ=255)
                        aaaCATCGTTGTTTCGATGTTTATGGGGGCGGTTTATGGCTACTTCCTCCATTATTGCCGCCGACTGTTTAAAGACAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCt                                                                                                                              >  7:275541/1‑149 (MQ=255)
                            aTCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGc                                                                                                                          >  3:10582/1‑149 (MQ=255)
                               gttgttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCa                                                                                                                        >  3:83615/1‑148 (MQ=255)
                                    ttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  >  3:260408/1‑149 (MQ=255)
                                     tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  <  8:56894/148‑1 (MQ=255)
                                      cGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                 <  3:276771/148‑1 (MQ=255)
                                       gATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCa                                                                                                               >  2:27052/1‑149 (MQ=255)
                                                gggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATa                                                                                                       <  8:12683/148‑1 (MQ=255)
                                                  gggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACg                                                                                                    >  6:55796/1‑149 (MQ=255)
                                                   ggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATACACCATTCAAATAACGt                                                                                                   >  7:159762/1‑149 (MQ=255)
                                                      ggTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTAAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCa                                                                                                <  6:61027/149‑1 (MQ=255)
                                                       gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                               <  7:315170/149‑1 (MQ=255)
                                                       gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                               <  4:10582/149‑1 (MQ=255)
                                                       gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                               <  3:311445/149‑1 (MQ=255)
                                                        tttATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCTGCAGGTAAAAGCCTGACTCTTATTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGGTTCGCCAAATACACAATTCAAATAACGTTCAAg                                                                                              >  2:221976/1‑149 (MQ=255)
                                                            tGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCTGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAgcgc                                                                                           <  4:83615/148‑1 (MQ=255)
                                                                tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTAAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTATTTAACTGGCTTCGCCAAATAAAACATTCAAATAACGATCAAGCGCAacac                                                                                      >  8:228186/1‑149 (MQ=255)
                                                                   ttCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcgc                                                                                    >  7:177646/1‑148 (MQ=255)
                                                                    tCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGATgccagtcgcgaaaagaagagtcaggcttttacctgctg                                                                                                               <  4:147257‑M2/120‑39 (MQ=255)
                                                                    tCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGATgccagtcgcgaaaagaagagtcaggcttttacctgctg                                                                                                               >  3:147257‑M2/1‑82 (MQ=255)
                                                                       tCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAAc                                                                                >  1:166119/1‑148 (MQ=255)
                                                                          attattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa                                                                            <  5:55796/149‑1 (MQ=255)
                                                                            tattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATGCAAATAACGTTCAAGCGCAACACGCGAACTAAAGc                                                                          >  5:79250/1‑149 (MQ=255)
                                                                              ttGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCa                                                                        <  3:288061/149‑1 (MQ=255)
                                                                                 ccgccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGc                                                                     >  1:325923/1‑149 (MQ=255)
                                                                                   gccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGACTGACTCTTCTTTTCGCGACTTTCATAGCAAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAACATTCAAATAAAGTTCAAGCGCAACACGCGAACTAAAGCCATGctg                                                                   <  8:206147/149‑3 (MQ=255)
                                                                                     cgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                 <  1:266220/149‑1 (MQ=255)
                                                                                      gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                 >  2:243494/1‑148 (MQ=255)
                                                                                      gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                <  4:239429/149‑1 (MQ=255)
                                                                                      gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                <  4:235275/149‑1 (MQ=255)
                                                                                         tGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAcc                                                             >  3:152617/1‑149 (MQ=255)
                                                                                          gTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc                                                                                                     <  8:259571/108‑1 (MQ=255)
                                                                                          gTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc                                                                                                     >  7:259571/1‑108 (MQ=255)
                                                                                          gTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCg                                                            >  4:136941/1‑149 (MQ=255)
                                                                                           tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCg                                                            >  3:120142/1‑148 (MQ=255)
                                                                                           tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCg                                                            >  7:222168/1‑148 (MQ=255)
                                                                                             ttgaacgttatttgaatggtttatttggcgaagccagttaaagaCGCGACTGGCGATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg                                                                    >  1:244240‑M1/45‑138 (MQ=255)
                                                                                             ttgaacgttatttgaatggtttatttggcgaagccagttaaagaCGCGACTGGCGATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg                                                                    <  2:244240‑M1/94‑1 (MQ=255)
                                                                                                 gTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCt                                                     >  2:171268/1‑149 (MQ=255)
                                                                                                     gCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgtg                                                 >  4:256030/1‑149 (MQ=255)
                                                                                                     gCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGAGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGTGATACCGTAATGCTCgtg                                                 <  8:275541/149‑1 (MQ=255)
                                                                                                      ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACt                                                                                                                                  <  8:222595/67‑1 (MQ=255)
                                                                                                      ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACt                                                                                                                                  >  7:222595/1‑67 (MQ=255)
                                                                                                      ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                >  3:270697/1‑133 (MQ=255)
                                                                                                      ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                <  4:270697/133‑1 (MQ=255)
                                                                                                            ccAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                          <  4:267334/133‑1 (MQ=255)
                                                                                                            ccAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                          >  3:267334/1‑133 (MQ=255)
                                                                                                                   gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGATgccagtcgcgaaaagaagagtcaggcttttacctgctggtaacggctgactttaaacagtcggcggcaataatcga                                                                         <  7:315585‑M2/111‑77 (MQ=255)
                                                                                                                   gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCt                                    >  4:180922/1‑148 (MQ=255)
                                                                                                                   gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTACAAGCGCAac                                                                                        >  1:142461/1‑96 (MQ=255)
                                                                                                                   gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTACAAGCGCAac                                                                                        <  2:142461/96‑1 (MQ=255)
                                                                                                                    tAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTa                                   >  3:93702/1‑148 (MQ=255)
                                                                                                                           cTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGc                           >  4:44996/1‑149 (MQ=255)
                                                                                                                              aCTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAc                                                              >  7:134834/1‑111 (MQ=255)
                                                                                                                              aCTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAc                                                              <  8:134834/111‑1 (MQ=255)
                                                                                                                                 cttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAt                     >  3:80835/1‑149 (MQ=255)
                                                                                                                                  ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  >  3:21503/1‑55 (MQ=255)
                                                                                                                                  ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  <  4:21503/55‑1 (MQ=255)
                                                                                                                                  ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt                    >  5:245145/1‑149 (MQ=255)
                                                                                                                                   tcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAATAAGGCGGAAATTGc                  >  8:160545/1‑149 (MQ=255)
                                                                                                                                       ttCGAGACTGTCATCGCGAGTCGCGTCTTTTACTGGCTTCGCCAAATATACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc                  <  6:91316/146‑1 (MQ=255)
                                                                                                                                        tCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTgg               <  4:93702/148‑1 (MQ=255)
                                                                                                                                          GCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa            <  6:252540‑M1/149‑1 (MQ=255)
                                                                                                                                          GCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa            <  8:16623‑M1/149‑1 (MQ=255)
                                                                                                                                          GCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa            >  4:260408‑M1/1‑149 (MQ=255)
                                                                                                                                           CGACTGGCGATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAAt           <  3:263767‑M1/149‑1 (MQ=255)
                                                                                                                                             ACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGa         <  1:142277‑M1/149‑1 (MQ=255)
                                                                                                                                              atGGCATCTCCAGTCGCGTCTTTAACTGGGTTCGCCAAATTATCAATTCAAATAAAGTTTAAGCTCAACACGCTAACTAAATCTATTCTTGATACATTAATGATCGTTTCTCTCTCCTGCTAAATAGAGCTGAAATTGCTGGTAATGAt        <  7:228186/148‑1 (MQ=255)
                                                                                                                                              CTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGAt        <  6:252651‑M1/149‑1 (MQ=255)
                                                                                                                                               TGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc       >  5:252651‑M1/1‑149 (MQ=255)
                                                                                                                                                 GCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCg      <  1:46771‑M1/148‑1 (MQ=255)
                                                                                                                                                   aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa   <  2:166119/149‑1 (MQ=255)
                                                                                                                                                   aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa   <  1:243494/149‑1 (MQ=255)
                                                                                                                                                    tCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTAAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGAACGCAAg  >  7:44686/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAG  >  NC_000913/2799487‑2799783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: