Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,799,636 C→T 7.5% intergenic (+23/+14) alaE → / ← ygaC alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,799,6360CT 7.5% 243.7 / 7.3 80intergenic (+23/+14)alaE/ygaCalanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (39/34);  new base T (2/4);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAG  >  NC_000913/2799489‑2799783
                                                                                                                                                   |                                                                                                                                                   
aGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCg                                                                                                                                                    <  7:12683‑M2/149‑2 (MQ=255)
    tATGGCGGCGGGCCGTTCAAACATCGTGGTTTCGATGTTGATGGGGGCGGGTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTGTAAAGTCTGCCGTTACCAGCAGGTAAAAGCCTGACTCTTATTTTCGCGACTGGCATCgcca                                                                                                                                                 >  2:240326‑M2/1‑144 (MQ=255)
      tGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCgccagtc                                                                                                                                              >  4:145084‑M2/1‑142 (MQ=255)
              gTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCttt                                                                                                                                      <  1:211512/149‑1 (MQ=255)
                 aGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCTGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTaa                                                                                                                                    <  8:265235/148‑1 (MQ=255)
                     cAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGCCTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGCCTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGc                                                                                                                               >  8:164600/1‑149 (MQ=255)
                      aaaCATCGTTGTTTCGATGTTTATGGGGGCGGTTTATGGCTACTTCCTCCATTATTGCCGCCGACTGTTTAAAGACAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCt                                                                                                                              >  7:275541/1‑149 (MQ=255)
                          aTCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGc                                                                                                                          >  3:10582/1‑149 (MQ=255)
                             gttgttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCa                                                                                                                        >  3:83615/1‑148 (MQ=255)
                                  ttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  >  3:260408/1‑149 (MQ=255)
                                   tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  <  8:56894/148‑1 (MQ=255)
                                    cGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                 <  3:276771/148‑1 (MQ=255)
                                     gATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCa                                                                                                               >  2:27052/1‑149 (MQ=255)
                                              gggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATa                                                                                                       <  8:12683/148‑1 (MQ=255)
                                                gggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACg                                                                                                    >  6:55796/1‑149 (MQ=255)
                                                 ggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATACACCATTCAAATAACGt                                                                                                   >  7:159762/1‑149 (MQ=255)
                                                    ggTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTAAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCa                                                                                                <  6:61027/149‑1 (MQ=255)
                                                     gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                               <  4:10582/149‑1 (MQ=255)
                                                     gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                               <  3:311445/149‑1 (MQ=255)
                                                     gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                               <  7:315170/149‑1 (MQ=255)
                                                      tttATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCTGCAGGTAAAAGCCTGACTCTTATTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGGTTCGCCAAATACACAATTCAAATAACGTTCAAg                                                                                              >  2:221976/1‑149 (MQ=255)
                                                          tGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCTGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAgcgc                                                                                           <  4:83615/148‑1 (MQ=255)
                                                              tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTAAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTATTTAACTGGCTTCGCCAAATAAAACATTCAAATAACGATCAAGCGCAacac                                                                                      >  8:228186/1‑149 (MQ=255)
                                                                 ttCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcgc                                                                                    >  7:177646/1‑148 (MQ=255)
                                                                  tCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGATgccagtcgcgaaaagaagagtcaggcttttacctgctg                                                                                                               >  3:147257‑M2/1‑82 (MQ=255)
                                                                  tCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGATgccagtcgcgaaaagaagagtcaggcttttacctgctg                                                                                                               <  4:147257‑M2/120‑39 (MQ=255)
                                                                     tCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAAc                                                                                >  1:166119/1‑148 (MQ=255)
                                                                        attattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa                                                                            <  5:55796/149‑1 (MQ=255)
                                                                          tattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATGCAAATAACGTTCAAGCGCAACACGCGAACTAAAGc                                                                          >  5:79250/1‑149 (MQ=255)
                                                                            ttGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCa                                                                        <  3:288061/149‑1 (MQ=255)
                                                                               ccgccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGc                                                                     >  1:325923/1‑149 (MQ=255)
                                                                                 gccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGACTGACTCTTCTTTTCGCGACTTTCATAGCAAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAACATTCAAATAAAGTTCAAGCGCAACACGCGAACTAAAGCCATGctg                                                                   <  8:206147/149‑3 (MQ=255)
                                                                                   cgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                 <  1:266220/149‑1 (MQ=255)
                                                                                    gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                 >  2:243494/1‑148 (MQ=255)
                                                                                    gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                <  4:235275/149‑1 (MQ=255)
                                                                                    gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                <  4:239429/149‑1 (MQ=255)
                                                                                       tGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAcc                                                             >  3:152617/1‑149 (MQ=255)
                                                                                        gTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc                                                                                                     >  7:259571/1‑108 (MQ=255)
                                                                                        gTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc                                                                                                     <  8:259571/108‑1 (MQ=255)
                                                                                        gTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCg                                                            >  4:136941/1‑149 (MQ=255)
                                                                                         tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCg                                                            >  7:222168/1‑148 (MQ=255)
                                                                                         tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCg                                                            >  3:120142/1‑148 (MQ=255)
                                                                                           ttgaacgttatttgaatggtttatttggcgaagccagttaaagaCGCGACTGGCGATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg                                                                    >  1:244240‑M1/45‑138 (MQ=255)
                                                                                           ttgaacgttatttgaatggtttatttggcgaagccagttaaagaCGCGACTGGCGATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg                                                                    <  2:244240‑M1/94‑1 (MQ=255)
                                                                                               gTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCt                                                     >  2:171268/1‑149 (MQ=255)
                                                                                                   gCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgtg                                                 >  4:256030/1‑149 (MQ=255)
                                                                                                   gCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGAGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGTGATACCGTAATGCTCgtg                                                 <  8:275541/149‑1 (MQ=255)
                                                                                                    ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACt                                                                                                                                  <  8:222595/67‑1 (MQ=255)
                                                                                                    ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACt                                                                                                                                  >  7:222595/1‑67 (MQ=255)
                                                                                                    ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                <  4:270697/133‑1 (MQ=255)
                                                                                                    ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                >  3:270697/1‑133 (MQ=255)
                                                                                                          ccAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                          <  4:267334/133‑1 (MQ=255)
                                                                                                          ccAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                          >  3:267334/1‑133 (MQ=255)
                                                                                                                 gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGATgccagtcgcgaaaagaagagtcaggcttttacctgctggtaacggctgactttaaacagtcggcggcaataatcga                                                                         <  7:315585‑M2/111‑77 (MQ=255)
                                                                                                                 gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCt                                    >  4:180922/1‑148 (MQ=255)
                                                                                                                 gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTACAAGCGCAac                                                                                        >  1:142461/1‑96 (MQ=255)
                                                                                                                 gTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTACAAGCGCAac                                                                                        <  2:142461/96‑1 (MQ=255)
                                                                                                                  tAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTa                                   >  3:93702/1‑148 (MQ=255)
                                                                                                                         cTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGc                           >  4:44996/1‑149 (MQ=255)
                                                                                                                            aCTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAc                                                              <  8:134834/111‑1 (MQ=255)
                                                                                                                            aCTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAc                                                              >  7:134834/1‑111 (MQ=255)
                                                                                                                               cttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAt                     >  3:80835/1‑149 (MQ=255)
                                                                                                                                ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  >  3:21503/1‑55 (MQ=255)
                                                                                                                                ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                  <  4:21503/55‑1 (MQ=255)
                                                                                                                                ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt                    >  5:245145/1‑149 (MQ=255)
                                                                                                                                 tcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAATAAGGCGGAAATTGc                  >  8:160545/1‑149 (MQ=255)
                                                                                                                                     ttCGAGACTGTCATCGCGAGTCGCGTCTTTTACTGGCTTCGCCAAATATACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc                  <  6:91316/146‑1 (MQ=255)
                                                                                                                                      tCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTgg               <  4:93702/148‑1 (MQ=255)
                                                                                                                                        GCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa            >  4:260408‑M1/1‑149 (MQ=255)
                                                                                                                                        GCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa            <  8:16623‑M1/149‑1 (MQ=255)
                                                                                                                                        GCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa            <  6:252540‑M1/149‑1 (MQ=255)
                                                                                                                                         CGACTGGCGATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAAt           <  3:263767‑M1/149‑1 (MQ=255)
                                                                                                                                           ACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGa         <  1:142277‑M1/149‑1 (MQ=255)
                                                                                                                                            atGGCATCTCCAGTCGCGTCTTTAACTGGGTTCGCCAAATTATCAATTCAAATAAAGTTTAAGCTCAACACGCTAACTAAATCTATTCTTGATACATTAATGATCGTTTCTCTCTCCTGCTAAATAGAGCTGAAATTGCTGGTAATGAt        <  7:228186/148‑1 (MQ=255)
                                                                                                                                            CTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGAt        <  6:252651‑M1/149‑1 (MQ=255)
                                                                                                                                             TGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc       >  5:252651‑M1/1‑149 (MQ=255)
                                                                                                                                               GCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCg      <  1:46771‑M1/148‑1 (MQ=255)
                                                                                                                                                 aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa   <  2:166119/149‑1 (MQ=255)
                                                                                                                                                 aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa   <  1:243494/149‑1 (MQ=255)
                                                                                                                                                  tCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTAAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGAACGCAAg  >  7:44686/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAG  >  NC_000913/2799489‑2799783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: