Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 594,606 G→A 8.2% S55S (AGC→AGT)  cusS ← copper‑sensing histidine kinase in two‑component regulatory system with CusR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913594,6060GA 8.2% 185.1 / 5.2 61S55S (AGC→AGT) cusScopper‑sensing histidine kinase in two‑component regulatory system with CusR
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base A (3/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

CACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGA  >  NC_000913/594461‑594753
                                                                                                                                                 |                                                                                                                                                   
cACCGGGGGAGTGATACACCGTTTCACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCt                                                                                                                                                   >  6:313202/1‑148 (MQ=255)
 aCCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTaa                                                                                                                                                 >  8:51359/1‑149 (MQ=255)
 aCCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTaa                                                                                                                                                 >  3:257781/1‑149 (MQ=255)
   cGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAAtc                                                                                                                                               >  5:251006/1‑149 (MQ=255)
     ggggAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTATTACCCGTTCAAGGGTGGCGCTAAtctc                                                                                                                                             <  4:90970/149‑1 (MQ=255)
       ggAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCtt                                                                                                                                           >  1:248014/1‑149 (MQ=255)
             aTACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAAt                                                                                                                                     >  1:7672/1‑149 (MQ=255)
             aTACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGAGTTAGTACCCGTTCAAGGGTGGCGCTAATCTCGTTTAAAt                                                                                                                                     >  8:278195/1‑149 (MQ=255)
                 acCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCAtt                                                                                                                                 <  5:37718/149‑1 (MQ=255)
                        tACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCAtt                                                                                                                                 >  6:47831/1‑142 (MQ=255)
                        tACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCAtt                                                                                                                                 <  5:47831/142‑1 (MQ=255)
                        tACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGGCGGGCTTGCGTTTCGTCCGGGGGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATc                                                                                                                           <  6:165914/148‑1 (MQ=255)
                          cccTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTg                                                                                                                        >  1:64144/1‑149 (MQ=255)
                                       ccAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCaaaa                                                                                                              <  2:248014/146‑1 (MQ=255)
                                       ccAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATg                                                                                                            <  4:235488/148‑1 (MQ=255)
                                         aGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAAc                                                                                                         >  3:75390/1‑149 (MQ=255)
                                         aGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGAGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAAc                                                                                                         >  2:212037/1‑149 (MQ=255)
                                          gggAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACt                                                                                                        >  2:330115/1‑149 (MQ=255)
                                            gAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACttt                                                                                                      <  4:311343/149‑1 (MQ=255)
                                            gAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACttt                                                                                                      >  8:173891/1‑149 (MQ=255)
                                               aTCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTAc                                                                                                   <  6:251006/149‑1 (MQ=255)
                                               aTCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTAc                                                                                                   >  2:177300/1‑149 (MQ=255)
                                                  aaCACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTTATAACCTGCTCGGCAAAATGAACTTTTACt                                                                                                  >  8:285264/1‑147 (MQ=255)
                                                  aaCACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACt                                                                                                  >  3:312006/1‑147 (MQ=255)
                                                    cacaTTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGt                                                                                              <  2:230556/149‑1 (MQ=255)
                                                           gAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCAt                                                                                       <  7:173891/149‑1 (MQ=255)
                                                             aTAACCACTGTCGATATCTTCCATCGTCATTAAGCGTCGGGCTTGAGGTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATg                                                                                      <  4:257781/148‑1 (MQ=255)
                                                             aTAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGa                                                                                     <  1:212037/149‑1 (MQ=255)
                                                                aCCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATc                                                                                   >  6:156317/1‑148 (MQ=255)
                                                                  cACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCAt                                                                                >  2:58556/1‑149 (MQ=255)
                                                                       aCGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCa                                                                             <  2:296193/147‑1 (MQ=255)
                                                                               ttCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGaaa                                                                    >  3:208534/1‑148 (MQ=255)
                                                                               ttCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGaaa                                                                    >  1:33828/1‑148 (MQ=255)
                                                                               ttCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGaaa                                                                    >  8:248894/1‑148 (MQ=255)
                                                                                     cGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAAcgccg                                                             >  8:16371/1‑149 (MQ=255)
                                                                                     cGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAAcgccg                                                             >  7:50895/1‑149 (MQ=255)
                                                                                       tCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAAcgccgc                                                            >  5:230937/1‑148 (MQ=255)
                                                                                          ttAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGAtg                                                        >  7:281185/1‑149 (MQ=255)
                                                                                                 tCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCa                                                 <  1:330115/149‑1 (MQ=255)
                                                                                                     gCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACCTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCt                                             >  8:246462/1‑149 (MQ=255)
                                                                                                         gCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGa                                           <  1:110735/147‑1 (MQ=255)
                                                                                                                gTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACCTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAg                                   <  7:246462/148‑1 (MQ=255)
                                                                                                                 tCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGt                                 >  4:45567/1‑149 (MQ=255)
                                                                                                                   aGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCaaa                                                                                                               >  2:48276/1‑69 (MQ=255)
                                                                                                                   aGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCaaa                                                                                                               <  1:48276/69‑1 (MQ=255)
                                                                                                                      gTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGc                            <  5:313202/149‑1 (MQ=255)
                                                                                                                      gTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGc                            <  7:86303/149‑1 (MQ=255)
                                                                                                                         aTTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCggg                         <  4:312006/149‑1 (MQ=255)
                                                                                                                              gTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGc                     <  7:16371/148‑1 (MQ=255)
                                                                                                                              gTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGc                     <  2:64144/148‑1 (MQ=255)
                                                                                                                              gTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGc                     <  7:99415/148‑1 (MQ=255)
                                                                                                                                 cccGTTCAAGGGTGGCACTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCAt                                                                                >  8:191414/1‑86 (MQ=255)
                                                                                                                                 cccGTTCAAGGGTGGCACTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCAt                                                                                >  8:191408/1‑86 (MQ=255)
                                                                                                                                 cccGTTCAAGGGTGGCACTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCAt                                                                                <  7:191414/86‑1 (MQ=255)
                                                                                                                                 cccGTTCAAGGGTGGCACTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCAt                                                                                <  7:191408/86‑1 (MQ=255)
                                                                                                                                     ttCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCATGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCTAAGGCGAAAAACGCCGCGATGGTGGCCAGGCGGATAAAAAAGGTCAGGCGGGTTGCCAGCGaa              >  2:97849/1‑148 (MQ=255)
                                                                                                                                       cAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGaaaa            <  4:75390/148‑1 (MQ=255)
                                                                                                                                        aaGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAAc           <  7:248894/148‑1 (MQ=255)
                                                                                                                                        aaGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACg          >  8:265870/1‑149 (MQ=255)
                                                                                                                                         aGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACgg         <  3:45567/149‑1 (MQ=255)
                                                                                                                                            gTGGCACTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGgcg      >  2:1628/1‑149 (MQ=255)
                                                                                                                                                 gcTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGa  >  6:71275/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACATTGGAATAACCACTGACGATATCTTCCAGCGTCATTAAGCGTCGGGCTTGCGTTTCGTCAGGGTGATTTAGTACCCGTTCAAGGGTGGCGCTAATCTCTTTTAAATCATTAATATCCTGCTCGGCAAAATGAACTTTTACTGAGTGGATCATGATCCATGCAAAGGCGAAAAACGCCGCGATGGTGGCCAGGCTGATAAAAAAGGTCAGGCGGGTTGCCAGCGAAAACGGGCGCTGA  >  NC_000913/594461‑594753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑