Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 895,325 T→C 5.1% I112T (ATC→ACC)  potG → putrescine ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913895,3250TC 5.1% 241.9 / 2.6 78I112T (ATC→ACC) potGputrescine ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (43/31);  new base C (2/2);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCC  >  NC_000913/895179‑895473
                                                                                                                                                  |                                                                                                                                                    
tATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGCACATCg                                                                                                                                                    >  1:262120/1‑149 (MQ=255)
    cTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCttt                                                                                                                                                <  4:143099/149‑1 (MQ=255)
                 aacaacCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAg                                                                                                                                   <  1:177239/149‑1 (MQ=255)
                    aacCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGa                                                                                                                                 >  3:131463/1‑148 (MQ=255)
                     acCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGAc                                                                                                                                >  3:177129/1‑148 (MQ=255)
                             ccGGACAGATACTGCTTGATGGCGTCGATTTGTCACAGGGTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTa                                                                                                                          <  2:106583/146‑1 (MQ=255)
                              cGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGa                                                                                                                      <  2:309599/149‑1 (MQ=255)
                                gACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGaaa                                                                                                                    >  6:42072/1‑149 (MQ=255)
                                  cAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGc                                                                                                                  <  8:274329/149‑1 (MQ=255)
                                      tAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATTATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACAGTGCAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGaa                                                                                                              <  7:117831/149‑1 (MQ=255)
                                       aaTGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATTTGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAGGCGGaaa                                                                                                             >  8:60691/1‑149 (MQ=255)
                                         tGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAAtt                                                                                                           <  4:131463/149‑1 (MQ=255)
                                           cTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGGCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGc                                                                                                         <  1:139873/149‑1 (MQ=255)
                                            ttGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGc                                                                                                         >  5:259823/1‑148 (MQ=255)
                                            ttGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGACCTGAAACAGGACAAACTACCGAAAGCGGAAATTGcc                                                                                                        >  2:116323/1‑149 (MQ=255)
                                              gATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCa                                                                                                       <  6:107554/148‑1 (MQ=255)
                                               aTGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGcc                                                                                                                                           >  4:164767/1‑111 (MQ=255)
                                               aTGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGcc                                                                                                                                           <  3:164767/111‑1 (MQ=255)
                                                 ggCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCg                                                                                                   >  5:126688/1‑149 (MQ=255)
                                                 ggCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCg                                                                                                   >  1:199379/1‑149 (MQ=255)
                                                  gCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCgg                                                                                                  <  1:98184/149‑1 (MQ=255)
                                                  gCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCgg                                                                                                  >  3:200792/1‑149 (MQ=255)
                                                   cGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCggg                                                                                                 >  8:297172/1‑149 (MQ=255)
                                                       gATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCaa                                                                                             >  8:66446/1‑149 (MQ=255)
                                                             tCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGAt                                                                                       <  7:42934/149‑1 (MQ=255)
                                                              cacaGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATTGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATg                                                                                      >  3:234583/1‑149 (MQ=255)
                                                               acaGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGTCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCAAGCCGGGTAAATTAGAtt                                                                                      >  8:314266/1‑147 (MQ=255)
                                                               acaGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTACGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGATACAGGACAAACTACCGATAGCGGAAATTGCCAGCCGGGTCATGGAGATGc                                                                                     >  4:250675/1‑149 (MQ=255)
                                                                 aGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGACCATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCCGCCGGGTCAATGAGATGCTc                                                                                   >  2:249077/1‑149 (MQ=255)
                                                                    ttCCGCCTTACCTGCGCCCCATCAATATGATGTTTCATTCTTACGCGCTGTTTCCGCATATGACCGTGGAACTGAAGATCGCTTTTGAGCTGACACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAAt                                                                                            <  7:166589/137‑1 (MQ=255)
                                                                    ttCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACACCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGc                                                                                >  2:41650/1‑149 (MQ=255)
                                                                    ttCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACACCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGc                                                                                >  2:36780/1‑149 (MQ=255)
                                                                    ttCCGCCTTACCTGCGCCACATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGACCTGAAACAGGACAAAATACCGAAAGCGGAAATTGCCAGCCGGGTCAAt                                                                                            >  8:166589/1‑137 (MQ=255)
                                                                       cGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTg                                                                             >  1:207126/1‑149 (MQ=255)
                                                                         ccTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGt                                                                           <  5:65378/149‑1 (MQ=255)
                                                                           ttACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTg                                                                          >  7:200829/1‑148 (MQ=255)
                                                                             aCCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCa                                                                        <  6:259823/148‑1 (MQ=255)
                                                                               cTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTAGGCCTggaatcatc                                                                     >  5:10176/1‑142 (MQ=255)
                                                                                  cggcccATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATTACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAACCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGAGCATATg                                                                    <  5:329595/144‑1 (MQ=255)
                                                                                   gcCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAg                                                                 <  8:200829/149‑1 (MQ=255)
                                                                                     cccATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAg                                                                 >  4:90950/1‑147 (MQ=255)
                                                                                     cccATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAg                                                                 >  4:291558/1‑147 (MQ=255)
                                                                                       cATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGt                                                             >  5:323947/1‑149 (MQ=255)
                                                                                        aTCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGtt                                                            >  3:267054/1‑149 (MQ=255)
                                                                                           aaTATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGc                                                         >  4:86588/1‑149 (MQ=255)
                                                                                                 atgTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACg                                                   >  4:261783/1‑149 (MQ=255)
                                                                                                    tttCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGt                                                                                                >  4:228960/1‑101 (MQ=255)
                                                                                                    tttCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGt                                                                                                <  3:228960/101‑1 (MQ=255)
                                                                                                    tttCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCaa                                                >  2:285133/1‑149 (MQ=255)
                                                                                                     ttCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCaaa                                               >  1:176040/1‑149 (MQ=255)
                                                                                                        aGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCaa                                                      <  4:219316/139‑1 (MQ=255)
                                                                                                        aGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCaa                                                      >  3:219316/1‑139 (MQ=255)
                                                                                                         gTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCg                                            >  5:100647/1‑148 (MQ=255)
                                                                                                           cTTACGCGCTGGTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCAt                                         <  6:220760/149‑1 (MQ=255)
                                                                                                            ttACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATc                                        >  2:17170/1‑149 (MQ=255)
                                                                                                              agtctcTGTTTCAGAATATGACCGTCTTACAGAACATCGCTTTTTGCATGAATCAGTTCTAAGTACCTAAATCGTAATTTGCGAGCCGGGTGAATGAGATGCTCGTCCTGGTGCATATGCAGTAGTTCGCCAAACGCAAACCGCAt                                         <  2:182150/141‑1 (MQ=255)
                                                                                                              aCGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCa                                       <  3:86588/148‑1 (MQ=255)
                                                                                                              aCGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCa                                       <  5:281100/148‑1 (MQ=255)
                                                                                                              aCGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCa                                       <  2:199379/148‑1 (MQ=255)
                                                                                                                gcgcTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTACGCCACCCGCAAACCGCATCAGCt                                    >  4:61798/1‑149 (MQ=255)
                                                                                                                   cTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTc                                 <  6:126688/149‑1 (MQ=255)
                                                                                                                         ccGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCggtgg                           >  8:263100/1‑149 (MQ=255)
                                                                                                                         ccGCATATGACCGTGGAACAGAACACCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCggtg                            <  1:36780/148‑1 (MQ=255)
                                                                                                                         ccGCATATGACCGTGGAACAGAACACCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCggtg                            <  1:41650/148‑1 (MQ=255)
                                                                                                                          cGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCggtggt                          <  5:42072/149‑1 (MQ=255)
                                                                                                                          cGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTCCATATGCAGGAGTTCGCCAAACGCACACCGCATCAGCTTTCCggtggt                          >  5:117982/1‑149 (MQ=255)
                                                                                                                           gCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCggtggt                          >  7:114319/1‑148 (MQ=255)
                                                                                                                            cATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCa                        <  2:176040/149‑1 (MQ=255)
                                                                                                                              tatGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGc                      <  7:66446/149‑1 (MQ=255)
                                                                                                                              tatGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGc                      >  3:36605/1‑149 (MQ=255)
                                                                                                                                tGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCg                     <  1:17170/148‑1 (MQ=255)
                                                                                                                                  aCCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGAc                   >  8:79880/1‑148 (MQ=255)
                                                                                                                                    cGTGGAACAGAACATCGCTTTTGACCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAAc                <  1:116323/149‑1 (MQ=255)
                                                                                                                                      tGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACgt              <  4:177129/149‑1 (MQ=255)
                                                                                                                                         aacagaacaTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGACAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGtgtg           >  1:85722/1‑149 (MQ=255)
                                                                                                                                          acagaacaTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGtgg          >  2:328245/1‑149 (MQ=255)
                                                                                                                                           cagaacaTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGtggc         >  2:50238/1‑149 (MQ=255)
                                                                                                                                                 aTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGtggccctggc   >  1:175060/1‑149 (MQ=255)
                                                                                                                                                  tCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGtggccctggcc  >  6:163663/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TATGCTGGCAGGTTTCGAACAACCTTCTGCCGGACAGATAATGCTTGATGGCGTCGATTTGTCACAGGTTCCGCCTTACCTGCGCCCCATCAATATGATGTTTCAGTCTTACGCGCTGTTTCCGCATATGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCC  >  NC_000913/895179‑895473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: