Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 939,974 C→T 9.5% L183L (CTG→TTG)  serS → seryl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913939,9740CT 9.5% 120.2 / 3.0 42L183L (CTG→TTG) serSseryl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (17/21);  new base T (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTA  >  NC_000913/939827‑940122
                                                                                                                                                   |                                                                                                                                                    
tttGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCt                                                                                                                                                     >  2:95324/1‑149 (MQ=255)
tttGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCt                                                                                                                                                     >  6:304128/1‑149 (MQ=255)
      gTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATAc                                                                                                                                               <  5:180214/149‑1 (MQ=255)
       ttCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATAcc                                                                                                                                              >  2:150493/1‑149 (MQ=255)
         cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGc                                                                                                                                                   >  7:311499/1‑142 (MQ=255)
         cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGc                                                                                                                                                   <  8:311499/142‑1 (MQ=255)
          gTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGaa                                                                                                                                           >  4:190933/1‑149 (MQ=255)
           tGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAAc                                                                                                                                          >  6:171584/1‑149 (MQ=255)
           tGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAAc                                                                                                                                          <  7:44979/149‑1 (MQ=255)
                         tGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGt                                                                                                                            >  8:221201/1‑149 (MQ=255)
                            gTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGt                                                                                                                            >  8:4825/1‑146 (MQ=255)
                             tGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGc                                                                                                                                  <  6:326006/139‑1 (MQ=255)
                             tGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGc                                                                                                                                  >  5:326006/1‑139 (MQ=255)
                                 aTGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTa                                                                                                                    <  6:54360/149‑1 (MQ=255)
                                  tGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTAt                                                                                                                   <  1:334885/149‑1 (MQ=255)
                                       tctGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTcc                                                                                                              >  2:127476/1‑149 (MQ=255)
                                       tctGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTcc                                                                                                              >  2:132137/1‑149 (MQ=255)
                                           gCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                                                                          <  8:96077/149‑1 (MQ=255)
                                                         gCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                                                                          >  5:87037/1‑135 (MQ=255)
                                                         gCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                                                                          <  6:87037/135‑1 (MQ=255)
                                                               gTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGATTGCGCTGGATCTGCATACCGACCAGCCTGGCTACAGTGAGAAGTATGTTCCGTACCTGGTTAACCAGGCCACGCt                                                                                      >  8:310531/1‑149 (MQ=255)
                                                                      tGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTgtacgg                                                                                >  4:153144/1‑148 (MQ=255)
                                                                        aCTGGTTCCCGCTTTGTGGTAATGAACGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTgtacggt                                                                               <  5:304128/147‑1 (MQ=255)
                                                                              tCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATTTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGCCACGCTGTCCGGAACGGGTc                                                                        >  2:51854/1‑148 (MQ=255)
                                                                              tCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATTTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTc                                                                        >  2:51851/1‑148 (MQ=255)
                                                                                          gTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGGTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAAtt                                                           >  6:180054/1‑149 (MQ=255)
                                                                                              tGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCt                                                       >  3:285988/1‑149 (MQ=255)
                                                                                                      cAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATTTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGCCACGCTGGACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCt                                               <  1:51854/149‑1 (MQ=255)
                                                                                                      cAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATTTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCt                                               <  1:51851/149‑1 (MQ=255)
                                                                                                         aTTGCTCGCATGCACCGCGCACTGGCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGtt                                            <  8:272790/149‑1 (MQ=255)
                                                                                                          ttGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTTGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTc                                           <  3:171925/149‑1 (MQ=255)
                                                                                                             cTCGCATGCACCGCGCACTGTCGGAGTTTATGGTGGATCTGCATACCGAACAGCATGGCTACTGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCa                                         <  6:84501/148‑1 (MQ=255)
                                                                                                             cTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCa                                         <  5:171584/148‑1 (MQ=255)
                                                                                                                gCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATAc                                      <  3:190933/148‑1 (MQ=255)
                                                                                                                   tGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTCGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGATCCATACTCGt                                  >  1:51577/1‑149 (MQ=255)
                                                                                                                        cgcgCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCt                             <  1:127476/149‑1 (MQ=255)
                                                                                                                        cgcgCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGGTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCt                             <  1:132137/149‑1 (MQ=255)
                                                                                                                            cACTGTCGCAGTTTACGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCTGgaa                         <  7:310531/149‑1 (MQ=255)
                                                                                                                                     aGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCa                <  1:150493/149‑1 (MQ=255)
                                                                                                                                          aTGCTGCATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCCGGACACGCTGTACGGTACGGGGCAACTGCCGAAATTTGCTGGCGATCTGTTCCCTACTCGTCCGCTGGAAGAAGAAGCAGaca            <  2:307013/148‑1 (MQ=255)
                                                                                                                                           tGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGATCCGTCCCTGGTTAACCAGGACACGCTGTACGGTACGTGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGacac           <  4:203744/148‑1 (MQ=255)
                                                                                                                                             cTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCa         <  3:153144/148‑1 (MQ=255)
                                                                                                                                                   cTGCATACCGAACAGCATGGCTACAGTGAGAACTATGATCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTCCTGGCTATCTGTTCCATACTCGTCCCCTGGAAGAAGAAGCAGACACCAGTAACTa  >  6:286743/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGGCGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTA  >  NC_000913/939827‑940122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑