Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,108,414 C→T 10.8% M176I (ATG→ATA)  opgC ← OPG biosynthetic transmembrane succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,108,4140CT10.8% 184.5 / 7.7 67M176I (ATG→ATA) opgCOPG biosynthetic transmembrane succinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (26/33);  new base T (4/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTAT  >  NC_000913/1108273‑1108556
                                                                                                                                             |                                                                                                                                              
gAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCgag                                                                                                                                          >  7:226266/1‑148 (MQ=255)
 aaCGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGaa                                                                                                                                        <  1:300733/149‑1 (MQ=255)
   cGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAAtt                                                                                                                                      >  7:29493/1‑149 (MQ=255)
      cAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTATTATTACCGCATAAACGATGCCGAGGCATAAAAAAATAACCGATATTTTTACCATCGAGAAttttt                                                                                                                                   >  3:56170/1‑149 (MQ=255)
        aaTAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTa                                                                                                                                 >  2:127311/1‑149 (MQ=255)
         aTAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTAt                                                                                                                                >  7:210087/1‑149 (MQ=255)
            aaaCAGCGTTTGCATGAAAATAAAATTTAACATTCCATTTATCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATTCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTc                                                                                                                              <  8:249297/148‑1 (MQ=255)
                   gTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTAt                                                                                                                       >  4:181271/1‑148 (MQ=255)
                       gCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGa                                                                                                                   <  3:181271/148‑1 (MQ=255)
                        cATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGaa                                                                                                                  <  3:281607/148‑1 (MQ=255)
                              aaTAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCt                                                                                                            >  7:170858/1‑148 (MQ=255)
                                tAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTaaa                                                                                                         >  3:1770/1‑149 (MQ=255)
                                       gAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTAttt                                                                                                   <  7:261916/148‑1 (MQ=255)
                                        aaCATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTc                                                                                                  <  5:117151/148‑1 (MQ=255)
                                        aaCATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTc                                                                                                  <  5:62528/148‑1 (MQ=255)
                                        aaCATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCATCTTATTCCCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTc                                                                                                  <  8:320581/148‑1 (MQ=255)
                                           aTGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGAt                                                                                              >  2:3032/1‑149 (MQ=255)
                                            tGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATg                                                                                             >  8:318585/1‑149 (MQ=255)
                                            tGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATg                                                                                             >  3:281053/1‑149 (MQ=255)
                                             gCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACTATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATgc                                                                                            >  5:121236/1‑149 (MQ=255)
                                                 ttACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCt                                                                                         >  2:214802/1‑148 (MQ=255)
                                                    cTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTACCAGAATTTTCTAAATTATTTCTGATGCGCTTaaa                                                                                     >  1:228449/1‑149 (MQ=255)
                                                     tCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTaaa                                                                                     >  8:255351/1‑148 (MQ=255)
                                                     tCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTaaa                                                                                     >  8:144360/1‑148 (MQ=255)
                                                       aGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAat                                                                                    <  1:127311/147‑1 (MQ=255)
                                                        gAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATc                                                                                 <  1:3032/149‑1 (MQ=255)
                                                        gAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATc                                                                                 <  7:189815/149‑1 (MQ=255)
                                                         actGGGCGGATACACAATAAAAATCGTTCTGCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATcc                                                                                <  4:172556/147‑1 (MQ=255)
                                                          aTGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGTCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATcc                                                                                <  2:52792/148‑1 (MQ=255)
                                                          aTGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAAACGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCa                                                                               <  5:254776/149‑1 (MQ=255)
                                                             ggCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATa                                                                             <  5:39098/148‑1 (MQ=255)
                                                              gaggATACACAATAAAAATCGTTCTTCTTATTACCGCTTAACCGATGCCGAGGCATAAAAAAAACACCGATAGTTTTAGTTTCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTTATGCGCTTAAATATACATaag                                                                           >  3:80143/3‑147 (MQ=255)
                                                              gCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACg                                                                           <  1:123296/149‑1 (MQ=255)
                                                                      acaATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGcgt                                                                   >  2:293344/1‑149 (MQ=255)
                                                                      acaATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGcgt                                                                   >  5:160836/1‑149 (MQ=255)
                                                                        aaTAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACGATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATAATTTCTGATGAGCTTAAATATCCATACGCACAGcgtcg                                                                 >  7:62029/1‑149 (MQ=255)
                                                                        aaTAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGAt                                                                                              <  1:275478/120‑1 (MQ=255)
                                                                        aaTAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGAt                                                                                              >  2:275478/1‑120 (MQ=255)
                                                                              aaTCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGa                                                           <  8:29493/149‑1 (MQ=255)
                                                                               aTCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGAc                                                          <  7:255351/149‑1 (MQ=255)
                                                                                        cttATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACTATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTaa                                                    <  8:94360/146‑1 (MQ=255)
                                                                                        cttATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAaca                                                  <  7:84290/148‑1 (MQ=255)
                                                                                           attaCCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACTATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAacac                                                 <  6:121236/146‑1 (MQ=255)
                                                                                           attaCCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCa                                               <  4:1770/148‑1 (MQ=255)
                                                                                           attaCCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCGTAAATATCCATACGCACAGCGTCGTCATGACCACTAacac                                                 <  8:170858/146‑1 (MQ=255)
                                                                                               ccGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTaa                                           >  8:268969/1‑148 (MQ=255)
                                                                                               ccGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTaa                                           <  1:293344/148‑1 (MQ=255)
                                                                                                cGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACTATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTaaa                                          >  4:259762/1‑148 (MQ=255)
                                                                                                 gCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTaaaaa                                        <  4:310730/149‑1 (MQ=255)
                                                                                                    tAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACca                                     <  7:144360/149‑1 (MQ=255)
                                                                                                     aaCCGATGCCGAGGCATAAAAAAATCACCGATAGGTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACca                                     >  6:267338/1‑148 (MQ=255)
                                                                                                      aCCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACcac                                    <  4:281053/148‑1 (MQ=255)
                                                                                                       ccGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAg                                  <  4:31213/149‑1 (MQ=255)
                                                                                                        cGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGCCCACTAACACCAGTAAAAACCACAgg                                 >  5:151551/1‑149 (MQ=255)
                                                                                                        cGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAgg                                 <  6:160836/149‑1 (MQ=255)
                                                                                                             ccGAGGCATAAAAAAATCACCGATAGTTTTAACATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGa                            <  4:56170/149‑1 (MQ=255)
                                                                                                              cGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGa                            >  2:265777/1‑148 (MQ=255)
                                                                                                                      aaaaaaaTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTc                   >  7:47802/1‑149 (MQ=255)
                                                                                                                      aaaaaaaTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCCGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTc                   >  1:58461/1‑149 (MQ=255)
                                                                                                                       aaaaaaTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTcc                  >  6:305149/1‑149 (MQ=255)
                                                                                                                         aaaaTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCa                <  8:226266/149‑1 (MQ=255)
                                                                                                                         aaaaTCACCGATAGTTTTACCATAGAGAATTTTTTATTCGTTTTACCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCa                <  2:228449/149‑1 (MQ=255)
                                                                                                                            aTCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGc             >  3:228126/1‑149 (MQ=255)
                                                                                                                                ccGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAAc         <  8:5901/149‑1 (MQ=255)
                                                                                                                                  gATAGTTTTACTATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGgt                                >  8:29172/1‑124 (MQ=255)
                                                                                                                                  gATAGTTTTACTATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGgt                                <  7:29172/124‑1 (MQ=255)
                                                                                                                                       ttttACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTACCACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGtattat  <  3:50142/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAATTATTTCTGATGCGCTTAAATATCCATACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTAT  >  NC_000913/1108273‑1108556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑