Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,328,762 C→T 7.8% W117* (TGG→TGA)  yciK ← putative EmrKY‑TolC system oxoacyl‑(acyl carrier protein) reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,328,7620CT 7.8% 155.5 / 2.9 51W117* (TGG→TGA) yciKputative EmrKY‑TolC system oxoacyl‑(acyl carrier protein) reductase
Reads supporting (aligned to +/- strand):  ref base C (27/20);  new base T (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

TGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGA  >  NC_000913/1328615‑1328909
                                                                                                                                                   |                                                                                                                                                   
tGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGc                                                                                                                                                     >  4:19013/1‑148 (MQ=255)
    tatGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGAc                                                                                                                                                >  6:257483/1‑149 (MQ=255)
      tGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCt                                                                                                                                              >  1:225242/1‑149 (MQ=255)
          caccAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCg                                                                                                                                           >  5:89662/3‑148 (MQ=255)
           cccAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGa                                                                                                                                         <  4:155752/149‑1 (MQ=255)
           cacAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTGCTGCCAGACCTGCGGa                                                                                                                                         >  7:177158/3‑149 (MQ=255)
                  ggCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGt                                                                                                                                   <  6:89662/148‑1 (MQ=255)
                   gCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGtt                                                                                                                                  <  5:257483/148‑1 (MQ=255)
                   gCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTc                                                                                                                                 <  7:104856/149‑1 (MQ=255)
                     tCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGc                                                                                                                               >  4:181613/1‑149 (MQ=255)
                       gTCCCTGACGTCCAACGCTTGATTAAGTAAAGAGCAGTGAACCGGCGTCCGATTTTAGTAATAACGGAAGCAGTGCCTGGGTGTGCATAAAGGTGGCATTAAGGTTGACGTGCTTGACGTCCTGCCAGTCCTGCGGATTTTGTTCGCt                                                                                                                              <  8:315088/148‑1 (MQ=255)
                             gACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTggg                                                                                                                       >  7:311732/1‑149 (MQ=255)
                              aCGGCCAACGCTTGATGACGTAACGACCAGTGAACCGGCGGCCGCTTTGAGTAATAAAGGCAGCAGTGCCTGGGGGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGGCCTGCCAGACCTGCGGATTTTGTTCGCTCATTgg                                                                                                                        <  3:179390/147‑1 (MQ=255)
                               cGTCCAACGCTTGATGAAGTAAAGACCAGTGACCCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGc                                                                                                                      <  3:183667/148‑1 (MQ=255)
                                    aaCGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACAt                                                                                                                >  2:29302/1‑149 (MQ=255)
                                              gaAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCaa                                                                                                      >  8:7028/1‑149 (MQ=255)
                                               aaGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATGTTGTTCGCTCATTGGGCAAACATCGCCGAGCAAt                                                                                                     >  3:101281/1‑149 (MQ=255)
                                                aGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGCAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATc                                                                                                    <  4:215798/149‑1 (MQ=255)
                                                           gTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGGCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGc                                                                                         <  3:19013/149‑1 (MQ=255)
                                                               aCCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCaaaa                                                                                     <  2:225242/149‑1 (MQ=255)
                                                                     gTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTAGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATGATGCAAAACACCag                                                                               >  2:62125/1‑148 (MQ=255)
                                                                               aGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCa                                                                                               >  1:164672/1‑123 (MQ=255)
                                                                               aGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCa                                                                                               <  2:164672/123‑1 (MQ=255)
                                                                                 taataaAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGa                                                                    >  7:97823/1‑148 (MQ=255)
                                                                                    taaAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGTCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAAt                                                                >  8:101849/1‑149 (MQ=255)
                                                                                    taaAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGTCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAAt                                                                >  8:106695/1‑149 (MQ=255)
                                                                                    taaAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAAt                                                                >  4:116093/1‑149 (MQ=255)
                                                                                             cAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATACGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCaa                                                       <  1:293193/149‑1 (MQ=255)
                                                                                              attGCCTGGGTGAGCATAAAGTTGGAATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAAt                                                      <  6:302275/147‑1 (MQ=255)
                                                                                              aGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAAt                                                      <  3:181613/149‑1 (MQ=255)
                                                                                                        tGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGc                                             >  3:144402/1‑148 (MQ=255)
                                                                                                          aGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTCGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCa                                           <  7:7028/148‑1 (MQ=255)
                                                                                                                   gTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAgttgt                                      >  5:157552/1‑144 (MQ=255)
                                                                                                                   gTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAgttgt                                      <  6:157552/144‑1 (MQ=255)
                                                                                                                    tGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCaa                                >  5:119631/1‑149 (MQ=255)
                                                                                                                      gCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAAtt                              >  2:258324/1‑149 (MQ=255)
                                                                                                                        aTTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAAtttt                            >  6:74617/1‑149 (MQ=255)
                                                                                                                              gTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACg                      >  8:300942/1‑149 (MQ=255)
                                                                                                                              gTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACg                      >  4:241998/1‑149 (MQ=255)
                                                                                                                                  aCCTGCATGACGTCCTGTCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCa                  <  7:101849/149‑1 (MQ=255)
                                                                                                                                  aCCTGCATGACGTCCTGTCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCa                  <  7:106695/149‑1 (MQ=255)
                                                                                                                                  aCCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCa                  <  8:177158/149‑1 (MQ=255)
                                                                                                                                       cATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGt               >  1:177125/1‑147 (MQ=255)
                                                                                                                                       cATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCa             <  4:144402/149‑1 (MQ=255)
                                                                                                                                         tGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCCGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAg            >  1:274401/1‑148 (MQ=255)
                                                                                                                                          gACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGc                   <  6:232648/140‑1 (MQ=255)
                                                                                                                                          gACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGc                   >  5:232648/1‑140 (MQ=255)
                                                                                                                                             gTCCTGCCAGACCTGCGGATTTTGTTCGCTCCTTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAAtttt                            >  1:61463/1‑128 (MQ=255)
                                                                                                                                             gTCCTGCCAGACCTGCGGATTTTGTTCGCTCCTTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAAtttt                            <  2:61463/128‑1 (MQ=255)
                                                                                                                                             gTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAAt       >  8:139927/1‑149 (MQ=255)
                                                                                                                                              tCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATc      >  8:103062/1‑149 (MQ=255)
                                                                                                                                               ccTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAGTCCGGCATTATGCAAAAAACCATCCAGACGCGGATATTTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGGGCAGGTCAGCAAATc      >  6:124326/1‑148 (MQ=255)
                                                                                                                                                   ccAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCgaga  <  5:74617/148‑1 (MQ=255)
                                                                                                                                                   ccAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCgag   <  2:177125/147‑1 (MQ=255)
                                                                                                                                                   ccAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCgag   <  8:311732/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCATATGCACCCCAGTTGGCTCGTCCCTGACGTCCAACGCTTGATGAAGTAAAGACCAGTGAACCGGCGTCCGATTTGAGTAATAAAGGAAGCAGTGCCTGGGTGAGCATAAAGGTGGCATTAACGTTGACCTGCATGACGTCCTGCCAGACCTGCGGATTTTGTTCGCTCATTGGGCAAACATCGCCGAGCAATCCGGCATTATGCAAAACACCATCCAGACGCGGATAATTAACGGCAATGCGCTGTGCCAGTTGTTGGCAATTTTCGGACGTGCAGGTCAGCAAATCGAGA  >  NC_000913/1328615‑1328909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑