Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,384,851 G→A 7.7% A245A (GCG→GCA)  ycjX → DUF463 family protein, puatative P‑loop NTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,384,8510GA 7.7% 154.4 / 3.2 52A245A (GCG→GCA) ycjXDUF463 family protein, puatative P‑loop NTPase
Reads supporting (aligned to +/- strand):  ref base G (27/20);  new base A (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTG  >  NC_000913/1384710‑1384999
                                                                                                                                             |                                                                                                                                                    
ttATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCg                                                                                                                                               <  4:108031/149‑1 (MQ=255)
     tcCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAg                                                                                                                                          >  2:26611/1‑149 (MQ=255)
       caccacTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCAAAACTGGCTCAGGCCGATAAGCa                                                                                                                                        >  2:39313/1‑149 (MQ=255)
        accacTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCa                                                                                                                                        >  6:119212/1‑148 (MQ=255)
          cacTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATAc                                                                                                                                     >  5:103085/1‑149 (MQ=255)
                    agaaGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCgg                                                                                                                            >  5:119609/4‑148 (MQ=255)
                    agCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCgg                                                                                                                            >  5:119614/1‑148 (MQ=255)
                     gCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGaa                                                                                                                          <  5:69219/149‑1 (MQ=255)
                     gCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGaa                                                                                                                          <  6:103085/149‑1 (MQ=255)
                             tGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTgcgc                                                                                                                  >  2:171970/1‑149 (MQ=255)
                              gCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGcgcg                                                                                                                 >  3:217766/1‑149 (MQ=255)
                               cACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTgcgc                                                                                                                  >  1:330988/1‑147 (MQ=255)
                                 cTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCAGTGGCCGGATGTCGATACGTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCTATGCCGGAATGCTGCGCGAgc                                                                                                              >  3:79967/1‑149 (MQ=255)
                                         aGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAat                                                                                                      >  4:115164/1‑149 (MQ=255)
                                          gCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAat                                                                                                      >  7:290350/1‑148 (MQ=255)
                                             tGGGCGCTTTGTCTTGCCGCGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAattat                                                                                                   >  6:306104/1‑148 (MQ=255)
                                                       gTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCgag                                                                                          >  8:318983/1‑147 (MQ=255)
                                                        tCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAg                                                                                       >  5:29963/1‑149 (MQ=255)
                                                            gCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAggtg                                                                                    <  1:171970/148‑1 (MQ=255)
                                                            gCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAggtg                                                                                    <  3:286769/148‑1 (MQ=255)
                                                            gCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAgg                                                                                      <  8:220345/146‑1 (MQ=255)
                                                             ccGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAggtggt                                                                                  >  3:31525/1‑149 (MQ=255)
                                                                ggAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGaa                                                                               <  6:166018/149‑1 (MQ=255)
                                                                  agaTATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAgg                                                                             <  7:318983/149‑1 (MQ=255)
                                                                    atatGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAgggg                                                                           <  5:119212/149‑1 (MQ=255)
                                                                          cAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCtat                                                                     <  5:65260/149‑1 (MQ=255)
                                                                           aGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCtata                                                                    <  6:29963/149‑1 (MQ=255)
                                                                                cgcCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGaa                                                                <  2:193842/148‑1 (MQ=255)
                                                                                    cgcgCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCAtt                                                           <  3:154697/149‑1 (MQ=255)
                                                                                     gcgcTGCAATTCTTCCCGTGGCCGTATGTCGATACCTGGGGCTAGTCCAAACTTGCGCAGGACGATAAGCATACCAATGCAGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGTTGAAGGGGTTCTATAAGAATCAttt                                                          >  6:134527/1‑149 (MQ=255)
                                                                                     gcgcTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGTCCGATAAGCATACAAATGCAGGAATGCTGCGCGAGCGCGTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCAttt                                                          >  6:139509/1‑149 (MQ=255)
                                                                                      cgcTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCAtttt                                                         <  1:26611/149‑1 (MQ=255)
                                                                                      cgcTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCAtttt                                                         <  5:262095/149‑1 (MQ=255)
                                                                                           cAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTg                                                      <  4:71251/147‑1 (MQ=255)
                                                                                           cAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTg                                                      <  4:217766/147‑1 (MQ=255)
                                                                                            aaTTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTgcgc                                                   >  5:177487/1‑149 (MQ=255)
                                                                                              ttcttcCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCtt                                                 >  1:296102/1‑149 (MQ=255)
                                                                                              ttcttcCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCtt                                                 >  1:33661/1‑149 (MQ=255)
                                                                                                    ccGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTCACCg                                           >  8:157747/1‑149 (MQ=255)
                                                                                                           cGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGAtt                                    >  5:210721/1‑149 (MQ=255)
                                                                                                                gTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATATGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCt                               >  2:313597/1‑149 (MQ=255)
                                                                                                                   gATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCCTACCAATGCCGGAAGGCTGCGCGAGAGCTTTAATTATTACTGCGAGAAGGTTGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCtggt                            <  4:79967/149‑1 (MQ=255)
                                                                                                                      aCCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGa                         >  1:291772/1‑149 (MQ=255)
                                                                                                                          ggggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAAATATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGc                     >  4:99960/1‑149 (MQ=255)
                                                                                                                             gCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTg                  >  4:2500/1‑149 (MQ=255)
                                                                                                                                aGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTTATTATTACTGCGATAAGGTTGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCaa               <  6:119609/149‑1 (MQ=255)
                                                                                                                                aGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCaa               <  6:119614/149‑1 (MQ=255)
                                                                                                                                 gTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAAc              <  3:2500/149‑1 (MQ=255)
                                                                                                                                 gTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAAc              >  7:211844/1‑149 (MQ=255)
                                                                                                                                  tCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAAcc             >  3:305255/1‑149 (MQ=255)
                                                                                                                                     aaaCTGGCGCAGGCCGATAAGCATACCAATGCCGGCATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACctct          >  7:46219/1‑149 (MQ=255)
                                                                                                                                       aCTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCa        <  2:330988/149‑1 (MQ=255)
                                                                                                                                             gcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTg  <  1:39313/149‑1 (MQ=255)
                                                                                                                                             gcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTg  <  4:305255/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTG  >  NC_000913/1384710‑1384999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑