Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,398,145 C→T 7.1% V160M (GTG→ATG)  uspE ← stress‑induced protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,398,1450CT 7.1% 167.6 / 2.8 56V160M (GTG→ATG) uspEstress‑induced protein
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base T (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

ATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCA  >  NC_000913/1398003‑1398291
                                                                                                                                              |                                                                                                                                                  
aTCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGccac                                                                                                                                              <  1:81604/149‑1 (MQ=255)
aTCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGGATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGCTACGGCTCTTCACTGGCGAGATTCACCGccac                                                                                                                                              <  6:139194/149‑1 (MQ=255)
 tCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGccac                                                                                                                                              >  5:268508/1‑148 (MQ=255)
         ttAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCtt                                                                                                                                     >  6:80214/1‑149 (MQ=255)
           aaTTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGc                                                                                                                                   >  2:217279/1‑149 (MQ=255)
            aTTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGc                                                                                                                                   <  1:291598/148‑1 (MQ=255)
                       cGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGcca                                                                                                                                               <  2:81832/125‑1 (MQ=255)
                       cGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGcca                                                                                                                                               >  1:81832/1‑125 (MQ=255)
                       cGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGc                                                                                                                        >  3:120171/1‑148 (MQ=255)
                         gATAAGCGCCAACCAGATGAACTTAGGTATGGTTGACTTGTTCTGACAGTTCGATCGTCTCTTTGCACAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGACACCAGCGCCTTGCCTACTTCCGGCCAc                                                                                                                     >  8:20280/1‑149 (MQ=255)
                             accgcCAACAAGATGATCTTCGGGATGGTTGACTTGTTCTGGCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGATCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCTCCTTGACTCCTTCTGGCCACGGc                                                                                                                  <  6:138184/146‑1 (MQ=255)
                                gcCAACCAGATGAACTTCGGTATGGTTGACGTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGt                                                                                                              <  5:44229/149‑1 (MQ=255)
                                        gATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTAATTGAGCGAATTATTATAAGGCTCTTCACTGGCGAGATTAACAGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGATGGTCTTTAAc                                                                                                       >  8:227362/1‑148 (MQ=255)
                                            aaCTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACTAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATc                                                                                                   <  3:103683/148‑1 (MQ=255)
                                            aaCTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATcc                                                                                                  <  4:279266/149‑1 (MQ=255)
                                                 cGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCacac                                                                                              <  6:268508/148‑1 (MQ=255)
                                                        gTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCtt                                                                                       <  4:48588/148‑1 (MQ=255)
                                                              ttGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCAtt                                                                                >  8:16410/1‑149 (MQ=255)
                                                                gTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTGACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGGCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGTCAttt                                                                               <  8:277576/148‑1 (MQ=255)
                                                                 ttCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCTCGGCTGTTCTTTCACCATCCACACCGGGCTTGGGCATTTGc                                                                             <  4:137527/149‑1 (MQ=255)
                                                                 ttCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGc                                                                             <  1:217279/149‑1 (MQ=255)
                                                                 ttCTGCCAGTTCGAGCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGc                                                                             <  1:15269/149‑1 (MQ=255)
                                                                      ccAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCCCCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCAGGTCTTTCACCATCCACACCGGGCTTGGGCCTTTGCGTaa                                                                         >  1:139076/1‑148 (MQ=255)
                                                                       cAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCAcc                                                                                                      >  1:136908/1‑118 (MQ=255)
                                                                       cAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCAcc                                                                                                      <  2:136908/118‑1 (MQ=255)
                                                                       cAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACa                                                                       <  3:299873/149‑1 (MQ=255)
                                                                             gATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGc                                                                  >  7:33433/1‑148 (MQ=255)
                                                                              aTCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCa                                                                <  4:116048/149‑1 (MQ=255)
                                                                               tCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGc                                                                             <  1:237152/135‑1 (MQ=255)
                                                                               tCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGc                                                                             >  2:237152/1‑135 (MQ=255)
                                                                                  tctcTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTcc                                                            >  7:120790/1‑149 (MQ=255)
                                                                                        tGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTg                                                        <  7:16410/147‑1 (MQ=255)
                                                                                         gACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGc                                                      <  5:296579/148‑1 (MQ=255)
                                                                                          aCCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCg                                                     >  6:109759/1‑148 (MQ=255)
                                                                                           ccAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGt                                                    >  3:134027/1‑148 (MQ=255)
                                                                                            cAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCg                                                     <  3:149192/146‑1 (MQ=255)
                                                                                            cAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCg                                                     >  4:149192/1‑146 (MQ=255)
                                                                                               tttttCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTaaaaa                                               >  3:39005/1‑149 (MQ=255)
                                                                                                 tttCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAAt                                              >  4:246770/1‑148 (MQ=255)
                                                                                                     aTTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCATCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCg                                         >  7:177339/1‑149 (MQ=255)
                                                                                                     aTTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCATCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCg                                         >  1:7415/1‑149 (MQ=255)
                                                                                                         aGCGCATTATGATACGGCTCTTCACTGGCGAGATTCATCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTc                                     <  2:7415/149‑1 (MQ=255)
                                                                                                         aGCGCATTATGATACGGCTCTTCACTGGCGAGATTCATCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTc                                     <  8:177339/149‑1 (MQ=255)
                                                                                                              aTTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGAc                                >  2:257951/1‑149 (MQ=255)
                                                                                                              aTTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGAc                                >  2:102898/1‑149 (MQ=255)
                                                                                                                   gATACGGATCTTCACTGGCCAGATTCACCGCCACCACCGACATGCCTCCTTCCGGCCACGGCTCGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGTCGTAAAAATCACCGCTTCCAGACGGTCa                           >  6:236492/1‑149 (MQ=255)
                                                                                                                    aTACGGATCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACTGATGCCAGTCCGTTGGCGTAAAAATGACGGCTTGCAGACGGTCa                           <  6:161073/148‑1 (MQ=255)
                                                                                                                     tACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAACTCACCGCTTCCAGCCGGTCATg                         >  2:272769/1‑149 (MQ=255)
                                                                                                                      agggCTCTTCACTGGCGAGATTCACCGCCACCTGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACAGCGGGGGTGGGCATTTGCGTAACAGATGGCAGTCCGTTGGCGTAATAATCACCGCTTCCAGTCGGTCATg                         <  2:139076/146‑1 (MQ=255)
                                                                                                                       cGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGtt                       <  4:132051/149‑1 (MQ=255)
                                                                                                                          ctctTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGt                    <  1:257951/149‑1 (MQ=255)
                                                                                                                                    gCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCAtttt          >  6:57107/1‑149 (MQ=255)
                                                                                                                                       agaTTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAg       >  6:192512/1‑149 (MQ=255)
                                                                                                                                        gaTTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGc      <  8:176388/149‑1 (MQ=255)
                                                                                                                                        gaTTCACCGCCACCAGCGCCTTGCCCCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAg       >  6:261108/1‑148 (MQ=255)
                                                                                                                                             aCCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGCTGCCAGTCCGTTGGCGTAAAAATCACCGCTTCGAGACGGTCATGTTGGTGCGCCATTTTTAGCACCa  >  3:68413/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCA  >  NC_000913/1398003‑1398291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑