Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,444,337 C→T 10.2% D429D (GAC→GAT)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,444,3370CT10.2% 110.9 / 3.7 39D429D (GAC→GAT) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (17/18);  new base T (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCGATGAAATCCGCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAC  >  NC_000913/1444189‑1444469
                                                                                                                                                    |                                                                                                                                    
tCGATGAAATCCGCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAc                                                                                                                                      <  6:152573/149‑1 (MQ=255)
  gATGAAATCCGCTGTCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGCACTGGGCGATTTCGTGCTGCATATGGCTGGGCTTGCGAATGATTTTCTCCCTGAcgc                                                                                                                                    >  8:249842/1‑149 (MQ=255)
  gATGAAATCCGCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgc                                                                                                                                    >  8:277251/1‑149 (MQ=255)
  gATGAAATCCGCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgc                                                                                                                                    >  1:234067/1‑149 (MQ=255)
         tCCGCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgctggccgc                                                                                                                             <  7:286170/149‑1 (MQ=255)
            gCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgctggccgctgg                                                                                                                          <  7:180770/149‑1 (MQ=255)
            gCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgctggccgctgg                                                                                                                          <  2:286650/149‑1 (MQ=255)
                gCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGt                                                                                                                      <  3:78905/149‑1 (MQ=255)
                 ccTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATTAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGATGCTGGCCGCTGGCAGTg                                                                                                                     >  6:242417/1‑149 (MQ=255)
                 ccTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGATGCTGGCCGCTGGCAGTg                                                                                                                     >  6:246907/1‑149 (MQ=255)
                 ccTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTg                                                                                                                     <  3:26853/149‑1 (MQ=255)
                   tttAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGgc                                                                                                                   >  7:22653/1‑149 (MQ=255)
                      aGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGgcgc                                                                                                                 >  3:286155/1‑148 (MQ=255)
                           gtgtAAAAGTCACCCAACGTGGGGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTgg                                                                                                           <  3:130005/149‑1 (MQ=255)
                               aaaaGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgctggcc                                                                                                                               <  6:184572/125‑1 (MQ=255)
                               aaaaGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGAcgctggcc                                                                                                                               >  5:184572/1‑125 (MQ=255)
                                  aGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAg                                                                                                    <  5:228252/149‑1 (MQ=255)
                                      aCCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAgg                                                                                                   >  1:3592/1‑146 (MQ=255)
                                      aCCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGa                                                                                                 <  2:3592/148‑1 (MQ=255)
                                        ccAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGtt                                                                                              >  3:48233/1‑149 (MQ=255)
                                         cAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTggg                                                                                                          <  2:331608/136‑1 (MQ=255)
                                         cAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTggg                                                                                                          >  1:331608/1‑136 (MQ=255)
                                         cAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGttt                                                                                             >  7:226816/1‑149 (MQ=255)
                                           aCGTTGGGTTGCCGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATTGCTTGGGTGGCGAATGATTTTCTCCCTGATGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTa                                                                                           <  5:242417/149‑1 (MQ=255)
                                           aCGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGATGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTa                                                                                           <  5:246907/149‑1 (MQ=255)
                                              tggtgTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTacac                                                                                        <  7:277251/149‑1 (MQ=255)
                                                   ttGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGAt                                                                                    <  1:190934/148‑1 (MQ=255)
                                                     gacggacgTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGaa                                                                                 >  1:9221/1‑149 (MQ=255)
                                                                      cATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCg                                                                <  8:22653/149‑1 (MQ=255)
                                                                               ggCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAg                                                       <  1:274564/149‑1 (MQ=255)
                                                                                 cgcATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCCCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGt                                                     <  3:213867/149‑1 (MQ=255)
                                                                                     ttcaaaTGAACGGTGCGATTTCTGTCTGCATATGGCTGGGGGGGCGAATGATTTTCTCCCTGCCGCGGGCCGCGGGCAGTGGCGCGACTGGTGAAAAGGGAGTGTTACACAGATGAATGCCACCTGGGATGTCGCAGGAAAAGGGGAGt                                                 <  8:129491/146‑1 (MQ=255)
                                                                                       aaaaTGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTg                                                <  8:226816/148‑1 (MQ=255)
                                                                                         aaTGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCa                                             <  5:83007/149‑1 (MQ=255)
                                                                                             aaCTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGAc                                         <  2:234067/149‑1 (MQ=255)
                                                                                                                   tatGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGAtc                   <  1:185703/149‑1 (MQ=255)
                                                                                                                    atggatggGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGc                                      <  2:203675/129‑1 (MQ=255)
                                                                                                                    atggatggGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGc                                      >  1:203675/1‑129 (MQ=255)
                                                                                                                          gggCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCAc            >  6:261387/1‑149 (MQ=255)
                                                                                                                                gCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGttt      >  2:248564/1‑149 (MQ=255)
                                                                                                                                gCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGttt      >  5:82126/1‑149 (MQ=255)
                                                                                                                                 cGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTc     >  2:171384/1‑149 (MQ=255)
                                                                                                                                 cGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTc     >  1:319960/1‑149 (MQ=255)
                                                                                                                                  gAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTc     >  7:304267/1‑148 (MQ=255)
                                                                                                                                   aaTGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGa   >  5:200127/1‑149 (MQ=255)
                                                                                                                                    aTGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAc  <  5:261387/149‑1 (MQ=255)
                                                                                                                                      gATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAc  >  1:29477/1‑147 (MQ=255)
                                                                                                                                                cTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGa                                  >  8:112431/1‑105 (MQ=255)
                                                                                                                                                    |                                                                                                                                    
TCGATGAAATCCGCTGGCCTTTAGCGGGTGTAAAAGTCACCCAACGTGGTGTTGACGGACGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAC  >  NC_000913/1444189‑1444469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑