Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,456,664 C→T 8.9% R79C (CGT→TGT)  paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,456,6640CT 8.9% 133.4 / 2.9 45R79C (CGT→TGT) paaEring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base C (19/22);  new base T (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CAGGAGGCGTATCGCTTTCGCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGC  >  NC_000913/1456520‑1456805
                                                                                                                                                |                                                                                                                                             
cAGGAGGCGTATCGCTTTCGCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGttt                                                                                                                                           >  6:32496/1‑149 (MQ=255)
    aggCGTATCGCTTTCGCCCCGGTCAACATTTTACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTcc                                                                                                                                       >  5:162356/1‑149 (MQ=255)
        gTATCGCTTTCGCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCt                                                                                                                                   <  6:138686/149‑1 (MQ=255)
             gCTTTAGCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGcc                                                                                                                              <  3:240634/149‑1 (MQ=255)
                   gCCCCGGTCAACATTTTACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGaa                                                                                                                        <  6:162356/149‑1 (MQ=255)
                   gCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGaa                                                                                                                        >  1:32008/1‑149 (MQ=255)
                   gCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGaa                                                                                                                        <  6:146491/149‑1 (MQ=255)
                    ccccGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGCGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAac                                                                                                                       <  7:121849/149‑1 (MQ=255)
                     cccGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAaca                                                                                                                      <  7:321553/149‑1 (MQ=255)
                      ccGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAacac                                                                                                                     <  8:295663/149‑1 (MQ=255)
                                   gACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTa                                                                                                        >  7:35927/1‑149 (MQ=255)
                                      cTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGATGTTTCTCCCGCTATGCCAGCGaa                                                                                                                        >  6:221474/1‑130 (MQ=255)
                                      cTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGATGTTTCTCCCGCTATGCCAGCGaa                                                                                                                        <  1:334043/130‑1 (MQ=255)
                                      cTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGATGTTTCTCCCGCTATGCCAGCGaa                                                                                                                        >  2:334043/1‑130 (MQ=255)
                                      cTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGATGTTTCTCCCGCTATGCCAGCGaa                                                                                                                        <  5:221474/130‑1 (MQ=255)
                                          aaaGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCt                                                                                                 <  1:210553/149‑1 (MQ=255)
                                           aaGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCGGCCAGGGTATGACGCTg                                                                                                <  5:287597/149‑1 (MQ=255)
                                           aaGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTg                                                                                                >  5:163726/1‑149 (MQ=255)
                                            aGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTgg                                                                                               <  1:149773/149‑1 (MQ=255)
                                              ccAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAg                                                                                             >  7:80925/1‑149 (MQ=255)
                                               cAGCTTTGATGGTGAGGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAgg                                                                                            >  7:232957/1‑149 (MQ=255)
                                                                aaTTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGtt                                                                                                                                            <  2:322244/84‑1 (MQ=255)
                                                                aaTTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGtt                                                                                                                                            >  1:322244/1‑84 (MQ=255)
                                                                aaTTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAgg                                                                             >  1:122470/1‑147 (MQ=255)
                                                                aaTTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAgg                                                                             <  2:122470/147‑1 (MQ=255)
                                                                 aTTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGc                                                                          <  5:32496/149‑1 (MQ=255)
                                                                     cggcGTTGTTACGCCATTTGCCGCAGCTATCTGCCTGGCTAAAATCGTGTGGCGGTGAAAGGTATTGAAGGCGGTCGTTTCTGCCGCTATGCCCGCGAACAGATCCGCGAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCAttt                                                                      <  1:278144/146‑1 (MQ=255)
                                                                       cctttggtACTCCATTAGCCGCAGCTATCTGACTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAATGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGTTCATGGTGCCGCAGGGGCATTTCg                                                                    <  6:32008/146‑1 (MQ=255)
                                                                       ccGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCg                                                                    >  1:68277/1‑149 (MQ=255)
                                                                        cGTTGTTACTCCATTTGCCGCAGCTATCTGACTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCgg                                                                   >  8:2485/1‑149 (MQ=255)
                                                                               aCTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAg                                                            >  3:68452/1‑149 (MQ=255)
                                                                                   cATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCg                                                         >  1:64622/1‑148 (MQ=255)
                                                                                     tttGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAg                                                      <  2:32008/149‑1 (MQ=255)
                                                                                        gCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGc                                                    <  8:35927/148‑1 (MQ=255)
                                                                                                tATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGc                                             >  2:249529/1‑147 (MQ=255)
                                                                                                 aTCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAg                                          >  7:162982/1‑149 (MQ=255)
                                                                                                      ccTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAgg                                         <  4:113062/145‑1 (MQ=255)
                                                                                                           cGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATc                                <  8:80925/149‑1 (MQ=255)
                                                                                                            gAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCt                               <  8:162982/149‑1 (MQ=255)
                                                                                                                    tgtgGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATg                                                                                                      >  7:233055/1‑70 (MQ=255)
                                                                                                                         cGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTgcag                    >  7:90375/1‑147 (MQ=255)
                                                                                                                                   cATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTa        >  3:238447/1‑149 (MQ=255)
                                                                                                                                     ttGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTAt       >  2:310685/1‑148 (MQ=255)
                                                                                                                                       gAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTa     <  2:68277/148‑1 (MQ=255)
                                                                                                                                         aGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGc  <  6:163726/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CAGGAGGCGTATCGCTTTCGCCCCGGTCAACATTTGACCTTAAAAGCCAGCTTTGATGGTGAAGAATTACGCCGTTGTTACTCCATTTGCCGCAGCTATCTGCCTGGCGAAATTAGTGTGGCGGTGAAAGCCATTGAAGGCGGACGTTTCTCCCGCTATGCCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGC  >  NC_000913/1456520‑1456805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑