Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,807,102	C→T	33.3%	P245S (CCG→TCG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,102	0	C	T	33.3%	79.1 / 55.6	66	P245S (CCG→TCG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (24/20);  new base T (9/13);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

ATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACA  >  NC_000913/1806956‑1807245                                                                                                                                                   |                                                                                                                                                aTCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCg                                                                                                                                               <  6:176203/149‑1 (MQ=255)  tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCCGGTGGTGCCACCACCgg                                                                                                                                              >  5:314290/1‑149 (MQ=255)      gAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGa                                                                                                                                           >  8:234541/1‑148 (MQ=255)        aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGaa                                                                                                                                          >  3:169118/1‑147 (MQ=255)            ccccAGCCGGACGATGTCCGCAAAGACGCGAAGGAAATCGTTAATAGCGGCAAGGCCAACCTGGTTGGCGTGTCGCTGGGTCCCCAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCCCCACCGGTGAAACGc                                                                                                                                      >  3:35567/2‑147 (MQ=255)            aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGc                                                                                                                                      >  1:145555/1‑147 (MQ=255)            aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGc                                                                                                                                      >  8:83037/1‑147 (MQ=255)            aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGc                                                                                                                                      >  1:316127/1‑147 (MQ=255)              ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa                                                                                                                                    >  1:110640/1‑147 (MQ=255)              ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTAAGGTTGTGCCACAACCGGTGAAACGCCcga                                                                                                                                  >  5:313175/1‑149 (MQ=255)                    ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCg                                                                                                                            >  6:221824/1‑149 (MQ=255)                      aCGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGtt                                                                                                                          <  3:128155/149‑1 (MQ=255)                       cGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTg                                                                                                                         <  8:179305/149‑1 (MQ=255)                         aTGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa                                                                                                                                    <  5:329040/136‑1 (MQ=255)                         aTGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa                                                                                                                                    >  6:329040/1‑136 (MQ=255)                         aTGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGgc                                                                                                                       >  5:173080/1‑149 (MQ=255)                          tGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAACCGGGGTGTCGTTTCCCTGGGTCCACACGGCGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGgcg                                                                                                                      >  2:75858/1‑149 (MQ=255)                             ccGCAAAGCCGCGCAGGAAATCGTTCATAGCGGCAAGGCCAAACGGGTTGTCGGTTCCCTGGGTCCACAATGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTTCCACCATCGGTGAAAAGCCAGAGTACCGTTGgcgcgg                                                                                                                   <  7:228516/149‑3 (MQ=255)                               gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                                                                                 >  3:163707/1‑149 (MQ=255)                               gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCGGGGTGGTGATAGTGAAAACGGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                                                                                 >  6:29446/1‑149 (MQ=255)                               gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGGCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTAGGCGCTGGt                                                                                                                 >  6:235850/1‑149 (MQ=255)                                 aaaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                                                                               <  7:130607/149‑1 (MQ=255)                                             gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCa                                                                                                          >  5:44772/1‑142 (MQ=255)                                             gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCa                                                                                                          <  6:44772/142‑1 (MQ=255)                                             gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCgg                                                                                                   >  6:10404/1‑149 (MQ=255)                                              aaaTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgc                                                                                                  <  2:145555/149‑1 (MQ=255)                                               aaTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgcg                                                                                                 <  4:248445/149‑1 (MQ=255)                                                   gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                             <  7:83037/149‑1 (MQ=255)                                                   gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                              <  5:221824/148‑1 (MQ=255)                                                   gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                              <  8:144282/148‑1 (MQ=255)                                                   gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGGCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                              <  5:296227/148‑1 (MQ=255)                                                    ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                             >  5:179106/1‑148 (MQ=255)                                                    ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                             >  1:19869/1‑148 (MQ=255)                                                      aaTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTGGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                           <  6:255222/148‑1 (MQ=255)                                                      aaTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                           <  6:173080/148‑1 (MQ=255)                                                       aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                                                                                         <  4:163707/149‑1 (MQ=255)                                                                  gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACAATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                               >  8:66047/1‑148 (MQ=255)                                                                   ccAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCa                                                                             <  3:277921/149‑1 (MQ=255)                                                                           gTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc                                                                     <  2:113510/149‑1 (MQ=255)                                                                                  tttCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAACTGCCTCTCtt                                                              >  4:201818/1‑149 (MQ=255)                                                                                      ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                           <  4:169118/148‑1 (MQ=255)                                                                                       cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                           >  7:97421/1‑147 (MQ=255)                                                                                       cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                           >  7:101996/1‑147 (MQ=255)                                                                                               acaAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTc                                                 <  2:110640/149‑1 (MQ=255)                                                                                                            ggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTa                                      <  5:192731/147‑1 (MQ=255)                                                                                                             gtgtTGATATTTTAAAGTGTATTGAGGTGGTGGGTCCTTCGGTGTTTTGCGTGAGTAGCGTTGGCGCTGGTGTGAGCATGGTCGGCGCGATGTCACTGATACTGGCAGAAAATGCCTCTCTTGAAGTGATGGTTCGTTTTGGCGTAGCt                                   <  6:246729/149‑1 (MQ=255)                                                                                                               gtTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc                                                                     <  6:280325/113‑1 (MQ=255)                                                                                                                ttGATAGTGAAAACTGTATTCAGGTGGTGCCACAACCGGTGAAAAGCCAGATTACCGTTGTCGCTGGTGACATCATGGTCTGCGCGATGACACTGAAACTGGAAGAAAATGCGTATCTTTAAGAGATGGTTCGATTTGGCGTAGCTGCg                                >  1:57170/1‑149 (MQ=255)                                                                                                                   aTAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                              <  2:316127/148‑1 (MQ=255)                                                                                                                   aTAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                               <  1:318579/147‑1 (MQ=255)                                                                                                                   aTAGTGAAAACTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                               >  2:318579/1‑147 (MQ=255)                                                                                                                    tAGTGAAAACTGTATTCAGGGGGTGCCTCCACCGGTGAAAAGCCAGTGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTGCGTTTTGGCGTAGCTGCGGGGa                            <  6:313175/149‑1 (MQ=255)                                                                                                                     aGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATCGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAg                           >  2:5826/1‑149 (MQ=255)                                                                                                                       tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                           <  7:289344/83‑1 (MQ=255)                                                                                                                       tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                           >  8:289344/1‑83 (MQ=255)                                                                                                                       tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                         >  3:213142/1‑149 (MQ=255)                                                                                                                         aaaaCTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAg                                                                            >  6:91093/1‑96 (MQ=255)                                                                                                                         aaaaCTGTATTCAGGTGGTGCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAg                                                                            <  5:91093/96‑1 (MQ=255)                                                                                                                           atcTGTATTCATGTGGTGCCACCATGGTTGAAAAGCCAGAGTACCGATGGCGCTGTTGACAGCATGGTCGGCGCGATGAGACTGAACCTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTTGCTGCGGGGAGTGCAg                      <  8:303195/146‑1 (MQ=255)                                                                                                                             cTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCca                   <  5:10404/149‑1 (MQ=255)                                                                                                                                  ttCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACt               >  1:210688/1‑148 (MQ=255)                                                                                                                                     aGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGCCAGCATGGGCGGCCCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCaa            <  5:235850/148‑1 (MQ=255)                                                                                                                                          gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAgg       >  4:98136/1‑148 (MQ=255)                                                                                                                                          gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAgg       <  7:234541/148‑1 (MQ=255)                                                                                                                                            gCCACCATCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGaa    >  4:148194/1‑149 (MQ=255)                                                                                                                                             ccaccaCCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACATCATGGTCTGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCTGGTAGTGCAGCCACACTCAATCAGGGAac   <  1:75858/149‑1 (MQ=255)                                                                                                                                              caccacCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACCCTCAATCAGGGAaca  >  1:264956/1‑149 (MQ=255)                                                                                                                                              caccacCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAaca  >  1:260526/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                ATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACA  >  NC_000913/1806956‑1807245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑