Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,862,926 C→G 7.8% G52G (GGC→GGG)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,862,9260CG 7.8% 166.2 / 3.8 50G52G (GGC→GGG) gapAglyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base C (26/20);  new base G (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAAAGTAGGTATCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCT  >  NC_000913/1862778‑1863072
                                                                                                                                                    |                                                                                                                                                   
tCAAAGTAGGTATCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGc                                                                                                                                                     >  7:4422/1‑149 (MQ=255)
         gTATCAACGGTTTTGGCCGTATCGGTCGCAGTGTTTTCCGTGCTGCTCAGAAACGTTCTGCCATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCCCTCACGGCCGGTTCGAc                                                                                                                                            <  4:80371/149‑1 (MQ=255)
            tCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGGCGTTTCGACgg                                                                                                                                          >  5:133188/1‑148 (MQ=255)
            tCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGGCGTTTCGACgg                                                                                                                                          >  7:189583/1‑148 (MQ=255)
            tCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACgg                                                                                                                                          >  1:203601/1‑148 (MQ=255)
                    tttGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTg                                                                                                                                  >  7:127794/1‑148 (MQ=255)
                      tGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGaa                                                                                                                                <  8:4422/148‑1 (MQ=255)
                      tGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGaa                                                                                                                                <  7:198557/148‑1 (MQ=255)
                        gCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGt                                                                                                                              <  8:276282/148‑1 (MQ=255)
                           gTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGa                                                                                                                            >  8:92578/1‑147 (MQ=255)
                            tATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGt                                                                                                                                    <  3:104768/138‑1 (MQ=255)
                            tATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGt                                                                                                                                    >  4:104768/1‑138 (MQ=255)
                               cGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACg                                                                                                                      <  5:278976/149‑1 (MQ=255)
                                  tCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGt                                                                                                                    >  5:267550/1‑148 (MQ=255)
                                      aTTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCt                                                                                                               >  8:292960/1‑149 (MQ=255)
                                                          gAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTCATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTTACGGT‑aaaaaaa                                                                                          >  3:159415/1‑149 (MQ=255)
                                                                  cTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCgtgt                                                                                   <  2:203601/148‑1 (MQ=255)
                                                                     aCATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTa                                                                                 <  6:267550/147‑1 (MQ=255)
                                                                        tCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCt                                                                            <  7:92578/149‑1 (MQ=255)
                                                                        tCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCt                                                                            >  2:255529/1‑149 (MQ=255)
                                                                           agaTCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGaa                                                                         >  8:175589/1‑149 (MQ=255)
                                                                           agaTCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGaa                                                                         >  8:90075/1‑149 (MQ=255)
                                                                            gaTCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAAc                                                                        >  3:117283/1‑149 (MQ=255)
                                                                                gTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGGCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGa                                                                    <  6:133188/149‑1 (MQ=255)
                                                                                gTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGGCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGa                                                                    <  8:189583/149‑1 (MQ=255)
                                                                                gTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTg                                                                     <  8:127794/148‑1 (MQ=255)
                                                                                     aaTCAACGACCTGTTAGACGCTGATTACATGGCATGCATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTTCCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCgg                                                               >  2:325976/1‑149 (MQ=255)
                                                                                     aaTCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATcc                                                                                       >  2:333301/1‑125 (MQ=255)
                                                                                          aCGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTg                                                                     >  8:57158/1‑138 (MQ=255)
                                                                                          aCGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTg                                                                     <  7:57158/138‑1 (MQ=255)
                                                                                          aCGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAAc                                                          >  3:119860/1‑149 (MQ=255)
                                                                                              ccTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTg                                                       >  7:260328/1‑148 (MQ=255)
                                                                                               cTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGa                                                      >  7:304230/1‑148 (MQ=255)
                                                                                                 gTTAGCCGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTCCCGCTGAACGTGATCCGGCTAACCTGAAAt                                                   >  8:62161/1‑149 (MQ=255)
                                                                                                  ttAGACGCTTATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGaaa                                                    <  1:80208/147‑1 (MQ=255)
                                                                                                    aGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATgg                                                 <  4:159415/148‑1 (MQ=255)
                                                                                                        gCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACg                                             >  4:36624/1‑148 (MQ=255)
                                                                                                            aTTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGaaa                                                    >  5:327379/1‑137 (MQ=255)
                                                                                                            aTTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGaaa                                                    <  6:327379/137‑1 (MQ=255)
                                                                                                            aTTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGtt                                        >  6:235864/1‑149 (MQ=255)
                                                                                                             ttACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTg                                       <  7:292960/149‑1 (MQ=255)
                                                                                                                    gCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGa                                <  7:176506/149‑1 (MQ=255)
                                                                                                                     catgcatgCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTTCCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGAc                               <  1:325976/149‑1 (MQ=255)
                                                                                                                      aTACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAAGGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACg                              <  5:285475/149‑1 (MQ=255)
                                                                                                                       tACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGt                             >  5:124937/1‑149 (MQ=255)
                                                                                                                                aaaTATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTg                     >  1:117217/1‑148 (MQ=255)
                                                                                                                                       aCTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAAc              >  1:105937/1‑149 (MQ=255)
                                                                                                                                           cACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAACTCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt          <  3:17644/148‑1 (MQ=255)
                                                                                                                                           cACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTc         <  7:90075/149‑1 (MQ=255)
                                                                                                                                                aCGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTc    >  3:214218/1‑149 (MQ=255)
                                                                                                                                                  ggCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCct  >  7:190420/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAAAGTAGGTATCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTCCGTGCTGCTCAGAAACGTTCTGACATCGAGATCGTTGCAATCAACGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGT‑AAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCT  >  NC_000913/1862778‑1863072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑