Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,049,040 C→T 7.2% T1368I (ACA→ATA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,049,0400CT 7.2% 167.2 / 2.7 56T1368I (ACA→ATA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (28/23);  new base T (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGAC  >  NC_000913/2048893‑2049188
                                                                                                                                                   |                                                                                                                                                    
aTACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTTATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACa                                                                                                                                                     <  4:26003/149‑1 (MQ=255)
aTACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTACCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACa                                                                                                                                                     <  4:26004/149‑1 (MQ=255)
    ccGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGCGGCCAGTCTGGAAAATGGTAGCTACACGCTTAGCACATCAATTAATGTCAACGCTGATTCGTCGACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGtct                                                                                                                                                 >  1:50442/1‑149 (MQ=255)
    ccGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCCCCACGCTTAGCACATCAATTAATGTAAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGact                                                                                                                                                 >  4:100728/1‑149 (MQ=255)
                aGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTACCTCCCCGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCgaga                                                                                                                                     >  8:73172/1‑149 (MQ=255)
                 gAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCgaga                                                                                                                                     <  6:109752/148‑1 (MQ=255)
                   aTCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACaa                                                                                                                                  >  7:93709/1‑149 (MQ=255)
                    tCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAAc                                                                                                                                 <  7:111021/149‑1 (MQ=255)
                        gAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGt                                                                                                                             <  7:26765/149‑1 (MQ=255)
                         aGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTc                                                                                                                            <  6:109358/149‑1 (MQ=255)
                              gaCCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGta                                                                                                                        >  8:77385/1‑148 (MQ=255)
                                  ggATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATATAGTCTCCGCAGGCGAGACAACCAGACTCTAAAtt                                                                                                                    >  8:240466/1‑148 (MQ=255)
                                  ggATACCGTTGAGGCAAGTCTGGAAAATGGTAGATCCACGCTTAGCCCATCAATTAATGTCAACGCTGATGCGTCTACTGCACATCTCACCTTGCTACAGGCACTTTTTGATATAGTCTCCGCAGGCGATACAACCAGTCTGTATAtt                                                                                                                    >  8:236033/1‑148 (MQ=255)
                                    aTACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAg                                                                                                                 <  6:106582/149‑1 (MQ=255)
                                               gCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACCTCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATaa                                                                                                      >  3:175623/1‑149 (MQ=255)
                                               gCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGTTGAAGGATaa                                                                                                      >  8:140259/1‑149 (MQ=255)
                                               gCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATaa                                                                                                      <  7:99669/149‑1 (MQ=255)
                                               gCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATaa                                                                                                      <  2:188244/149‑1 (MQ=255)
                                               gCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATaa                                                                                                      <  4:175107/149‑1 (MQ=255)
                                                ccAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAAt                                                                                                     <  2:217118/149‑1 (MQ=255)
                                                ccAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAAt                                                                                                     <  2:289722/149‑1 (MQ=255)
                                                  aGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTa                                                                                                   <  1:184402/149‑1 (MQ=255)
                                                                      cACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCcag                                                                                >  3:299571/1‑148 (MQ=255)
                                                                       aCGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCcagca                                                                              <  8:93709/149‑1 (MQ=255)
                                                                       aCGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCcag                                                                                >  6:48218/1‑147 (MQ=255)
                                                                          cTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCagga                                                                           <  1:302497/149‑1 (MQ=255)
                                                                           ttAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCaggag                                                                          >  3:22539/1‑149 (MQ=255)
                                                                                 caTCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCCCCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAAcc                                                                    >  4:235181/1‑149 (MQ=255)
                                                                                 caTCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAAcc                                                                    <  7:73172/149‑1 (MQ=255)
                                                                                  aTCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATAAAGTCTCCGCAGGCGAGACAACAAGACTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAAccc                                                                   >  5:305610/1‑149 (MQ=255)
                                                                                   tCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCt                                                                  >  6:52629/1‑149 (MQ=255)
                                                                                       ttAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGc                                                              >  2:295747/1‑149 (MQ=255)
                                                                                               aaCGCTGATGCGTCTACGGCACATCTCCCCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCAc                                                       >  2:158927/1‑148 (MQ=255)
                                                                                               aaCGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCAc                                                       >  8:232043/1‑148 (MQ=255)
                                                                                                    tGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGt                                                  >  2:12544/1‑148 (MQ=255)
                                                                                                    tGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGt                                                  >  7:114108/1‑148 (MQ=255)
                                                                                                           tCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGt                                          <  4:299571/149‑1 (MQ=255)
                                                                                                                ggCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCACCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAcca                                     >  2:232722/1‑148 (MQ=255)
                                                                                                                     aTCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAg                                 >  3:248990/1‑148 (MQ=255)
                                                                                                                      tctcACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGt                                >  6:151035/1‑148 (MQ=255)
                                                                                                                          aCCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGtaataa                           >  6:246254/1‑149 (MQ=255)
                                                                                                                            cTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGt                                                            <  6:82820/114‑1 (MQ=255)
                                                                                                                            cTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGt                                                            >  5:82820/1‑114 (MQ=255)
                                                                                                                            cTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGt                                          <  7:244086/132‑1 (MQ=255)
                                                                                                                            cTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGt                                          >  8:244086/1‑132 (MQ=255)
                                                                                                                                   cAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtata                    <  3:262616/147‑1 (MQ=255)
                                                                                                                                     ggCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCACCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatatata                <  5:246254/149‑1 (MQ=255)
                                                                                                                                      gCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtac               <  7:232043/149‑1 (MQ=255)
                                                                                                                                        aCTTTTTGATATAGTCTGCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtacta             <  7:240466/149‑1 (MQ=255)
                                                                                                                                        aCTTTTTGATATAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtacta             <  7:236033/149‑1 (MQ=255)
                                                                                                                                        aCTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtacta             >  7:122082/1‑149 (MQ=255)
                                                                                                                                         cTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtactac            <  1:158927/149‑1 (MQ=255)
                                                                                                                                          tttttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtactac            >  5:184474/1‑148 (MQ=255)
                                                                                                                                          tttttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtactac            >  6:252890/1‑148 (MQ=255)
                                                                                                                                            tttGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTaccaa         >  3:116494/1‑149 (MQ=255)
                                                                                                                                                aTACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCac     >  8:50617/1‑149 (MQ=255)
                                                                                                                                                   cAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacgac  >  3:102748/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGAC  >  NC_000913/2048893‑2049188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑