Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,094,624 C→T 9.3% S30S (AGC→AGT)  hisH → imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,094,6240CT 9.3% 126.0 / 3.3 43S30S (AGC→AGT) hisHimidazole glycerol phosphate synthase, glutamine amidotransferase subunit
Reads supporting (aligned to +/- strand):  ref base C (17/22);  new base T (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

CTGCGCCAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGG  >  NC_000913/2094476‑2094771
                                                                                                                                                    |                                                                                                                                                   
cTGCGCCAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGc                                                                                                                                                     <  4:195194/149‑1 (MQ=255)
 tGCGCCAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGcc                                                                                                                                                    >  2:100489/1‑149 (MQ=255)
   cgcCAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGt                                                                                                                                                  <  1:158911/149‑1 (MQ=255)
      cAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAGCGTGGTGCTCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCCCGGTTATGAACCCAAAGTCAGCCGTGAc                                                                                                                                               >  1:164309/1‑149 (MQ=255)
      cAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGAc                                                                                                                                               >  8:287560/1‑149 (MQ=255)
           cATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGTCGTGACCCGGa                                                                                                                                          >  1:151055/1‑149 (MQ=255)
           cATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGa                                                                                                                                          <  4:221756/149‑1 (MQ=255)
                gcgTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGTCGTGACCCGGAcgtcg                                                                                                                                     <  2:151055/149‑1 (MQ=255)
                 cgTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGGGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGAcgtcgt                                                                                                                                    <  5:152897/149‑1 (MQ=255)
                      aaGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGc                                                                                                                               <  8:43898/149‑1 (MQ=255)
                           gATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGcc                                                                                                                          >  8:127159/1‑149 (MQ=255)
                            aTACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCg                                                                                                                         <  2:66125/149‑1 (MQ=255)
                              aCCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGa                                                                                                                        >  8:255/1‑148 (MQ=255)
                               cccTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCGGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATa                                                                                                                      <  6:149633/149‑1 (MQ=255)
                                    cccTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACt                                                                                                                  <  7:318916/148‑1 (MQ=255)
                                       tcgtcgAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGttt                                                                                                              >  5:66655/1‑149 (MQ=255)
                                         gtcgAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGtttt                                                                                                             <  7:255/148‑1 (MQ=255)
                                         gtcgAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGttt                                                                                                              <  2:55193/147‑1 (MQ=255)
                                          tcgAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTa                                                                                                           >  5:126746/1‑149 (MQ=255)
                                          tcgAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTa                                                                                                           <  6:52363/149‑1 (MQ=255)
                                                   gTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGtt                                                                                                  <  6:126746/149‑1 (MQ=255)
                                                        gTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACACAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGTCGTTGGCAc                                                                                             >  6:266606/1‑149 (MQ=255)
                                                           aTGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTgc                                                                                          <  2:18197/149‑1 (MQ=255)
                                                                 gtggtgATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAgc                                                                                    <  4:305014/149‑1 (MQ=255)
                                                                       aTCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGa                                                                               <  8:182624/148‑1 (MQ=255)
                                                                       aTCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGAt                                                                              <  7:287560/149‑1 (MQ=255)
                                                                                aCCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAgg                                                                      >  3:295195/1‑148 (MQ=255)
                                                                                        cgcCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAgc                                                             >  6:162833/1‑149 (MQ=255)
                                                                                         gcCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAgcg                                                            >  7:93555/1‑149 (MQ=255)
                                                                                               cTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCt                                                      <  7:127159/149‑1 (MQ=255)
                                                                                                tGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGTATCAGGTACGTGAGCGCGAGCTg                                                     >  6:23933/1‑149 (MQ=255)
                                                                                                       ggTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGAtc                                              >  3:199177/1‑149 (MQ=255)
                                                                                                       ggTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGAtc                                              >  2:266344/1‑149 (MQ=255)
                                                                                                                 gCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCaaa                                      <  3:72356/147‑1 (MQ=255)
                                                                                                                 gCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCaaa                                      >  4:72356/1‑147 (MQ=255)
                                                                                                                   cATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGttt                                                  >  3:107654/1‑133 (MQ=255)
                                                                                                                   cATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGttt                                                  <  4:107654/133‑1 (MQ=255)
                                                                                                                        cgcgTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTAcc                             >  1:177577/1‑149 (MQ=255)
                                                                                                                         gcgTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGa                                                         <  7:213908/120‑1 (MQ=255)
                                                                                                                         gcgTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGa                                                         >  8:213908/1‑120 (MQ=255)
                                                                                                                                     tGAACCCAAAGTCAGTCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGt                                >  6:267082/1‑133 (MQ=255)
                                                                                                                                     tGAACCCAAAGTCAGTCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGt                                <  5:267082/133‑1 (MQ=255)
                                                                                                                                                tCAGCCGTGAACCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGAGGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTa     <  5:266606/149‑1 (MQ=255)
                                                                                                                                                  aGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAg    <  6:66655/148‑1 (MQ=255)
                                                                                                                                                   gCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAggg  <  2:177577/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGCGCCAGGCCATCCGCGTGGAAGGCGATACCCTGCCCTCGTCGAAAGGAGTGCTGTAATGAACGTGGTGATCCTTGATACCGGCTGCGCCAACCTGAACTCGGTGAAGTCTGCCATTGCGCGTCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACTGCGCAAGCGGCGATGGATCAGGTACGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGG  >  NC_000913/2094476‑2094771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑