Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,223,493 G→A 5.6% A437T (GCT→ACT)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,4930GA 5.6% 226.5 / 2.5 72A437T (GCT→ACT) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base A (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

ATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTG  >  NC_000913/2223345‑2223630
                                                                                                                                                    |                                                                                                                                         
aTACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTcgccg                                                                                                                                           <  7:204860/149‑1 (MQ=255)
          catcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGgcg                                                                                                                                  >  1:36750/1‑148 (MQ=255)
          catcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGgcg                                                                                                                                  >  7:19520/1‑148 (MQ=255)
           atcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCgca                                                                                                                                <  7:171992/149‑1 (MQ=255)
            tcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCgcag                                                                                                                               >  6:255311/1‑149 (MQ=255)
              tgctgTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCgcagc                                                                                                                              >  7:311279/1‑148 (MQ=255)
                     aaaaaTGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGt                                                                                                                       >  3:52354/1‑148 (MQ=255)
                      ataaTGGCGGGCGATGGAGTGGGAGAAGCCATATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTAAGCAGGAGGAAGTAAGCATAGCGTTTTTACACCGTTTCGCCTCTGCTGGCGAAGCAATTCGtt                                                                                                                      <  4:268465/146‑1 (MQ=255)
                      aaaaTGGAGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACTCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGtt                                                                                                                      <  2:158665/148‑1 (MQ=255)
                       aaaTGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTAt                                                                                                                    >  8:117385/1‑149 (MQ=255)
                           ggcgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAgg                                                                                                                >  4:90388/1‑149 (MQ=255)
                              gggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCgg                                                                                                             >  6:324812/1‑149 (MQ=255)
                               ggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGt                                                                                                            <  1:282790/149‑1 (MQ=255)
                                   aTGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGACCGAACGCGATTACTTTGTCTGTACGCCGCAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTAATCAGGCGGTGCAt                                                                                                        >  8:158410/1‑149 (MQ=255)
                                      gCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTc                                                                                                      <  7:100599/148‑1 (MQ=255)
                                              gAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGt                                                                                             >  2:78507/1‑149 (MQ=255)
                                               aaGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTc                                                                                            >  5:1186/1‑149 (MQ=255)
                                                 gCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCg                                                                                           >  2:140809/1‑148 (MQ=255)
                                                  ccAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGaa                                                                                         <  1:78507/149‑1 (MQ=255)
                                                  ccAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGaa                                                                                         <  8:311279/149‑1 (MQ=255)
                                                    aaaTCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGa                                                                                       >  2:149740/1‑149 (MQ=255)
                                                    aaaTCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGa                                                                                       <  2:212466/149‑1 (MQ=255)
                                                     aaTCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGa                                                                                       >  2:8559/1‑148 (MQ=255)
                                                            gCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTg                                                                                <  4:52354/148‑1 (MQ=255)
                                                              tggtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCg                                                                             >  4:90027/1‑149 (MQ=255)
                                                                 tggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTg                                                                          >  6:322441/1‑149 (MQ=255)
                                                                 tggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTg                                                                          >  1:52015/1‑149 (MQ=255)
                                                                  ggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTgg                                                                         <  2:190037/149‑1 (MQ=255)
                                                                   gATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGa                                                                        >  8:298293/1‑149 (MQ=255)
                                                                    attattTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCACTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGa                                                                        >  7:231081/1‑148 (MQ=255)
                                                                    attattTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCACTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGa                                                                        >  1:197826/1‑148 (MQ=255)
                                                                     ttattTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGgta                                                                      >  3:125742/1‑149 (MQ=255)
                                                                            aaaCAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGctct                                                               >  4:211744/1‑149 (MQ=255)
                                                                            aaaCAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGctct                                                               >  1:317091/1‑149 (MQ=255)
                                                                               cAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTgcg                                                            <  8:19520/149‑1 (MQ=255)
                                                                               cAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTgcg                                                            <  3:90388/149‑1 (MQ=255)
                                                                               cAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTgcg                                                            <  2:36750/149‑1 (MQ=255)
                                                                               cAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTgcg                                                            >  2:256179/1‑149 (MQ=255)
                                                                                   ccGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTgcggcg                                                         >  5:150856/1‑148 (MQ=255)
                                                                                    cGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGt                                                        >  8:323347/1‑148 (MQ=255)
                                                                                           gATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGacac                                                >  1:20141/1‑149 (MQ=255)
                                                                                               tCTTCGTCTCTACGCCGGAGGAAGGCAGCAAAGCCTCTTTACACCGTTTCGCCGCCGCGGCCGCAGCAATTCGTTCTCCGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGa                                             <  2:6562/148‑1 (MQ=255)
                                                                                                cTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTATCGACACCGAg                                            <  5:253851/148‑1 (MQ=255)
                                                                                                cTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTATCGACACCGAg                                            >  6:253851/1‑148 (MQ=255)
                                                                                                cTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAg                                            <  7:323347/148‑1 (MQ=255)
                                                                                                cTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAg                                            <  6:150856/148‑1 (MQ=255)
                                                                                                cTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGt                                           >  1:92084/1‑149 (MQ=255)
                                                                                                  ttGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTgg                                         >  2:4974/1‑149 (MQ=255)
                                                                                                   tGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGt                                           <  1:8559/146‑1 (MQ=255)
                                                                                                   tGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTg                                          <  5:322441/147‑1 (MQ=255)
                                                                                                    gTCTGTACGCCGGAGGAAGGCAGCAAAGCTTTTTTACACAGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGt                                        <  4:281555/148‑1 (MQ=255)
                                                                                                     tCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTa                                       <  6:1186/148‑1 (MQ=255)
                                                                                                     tCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTAt                                      <  7:272585/149‑1 (MQ=255)
                                                                                                               ggaggaAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGGTATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTAc                            <  4:125742/149‑1 (MQ=255)
                                                                                                                 aggaAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTAccg                          <  7:117385/149‑1 (MQ=255)
                                                                                                                   gaAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTAccgcc                        >  6:313727/1‑149 (MQ=255)
                                                                                                                     aGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTaa                                                      >  3:50084/1‑117 (MQ=255)
                                                                                                                     aGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTaa                                                      <  4:50084/117‑1 (MQ=255)
                                                                                                                       gcagcaAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTAccgccg                       >  6:2576/1‑146 (MQ=255)
                                                                                                                          gcaAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATc                 <  2:52015/149‑1 (MQ=255)
                                                                                                                             aaGCGTTTTTACACCGTTTCGCCACTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGAc              <  8:231081/149‑1 (MQ=255)
                                                                                                                             aaGCGTTTTTACACCGTTTCGCCACTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGAc              <  2:197826/149‑1 (MQ=255)
                                                                                                                                cGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGAcagc           >  8:50239/1‑149 (MQ=255)
                                                                                                                                cGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGAcagc           <  5:324812/149‑1 (MQ=255)
                                                                                                                                       acacCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTg     <  2:24444/148‑1 (MQ=255)
                                                                                                                                       acacCGTTTCGCCGCTGCGGGCGCAGCAATTAGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGGGTTGGATATCGCTATGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCTCCGGAGATCGACAGCCAGCTgg    >  5:93495/1‑149 (MQ=255)
                                                                                                                                        cacCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTgg    <  3:90027/148‑1 (MQ=255)
                                                                                                                                        cacCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGt   <  2:92084/149‑1 (MQ=255)
                                                                                                                                        cacCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGt   <  2:20141/149‑1 (MQ=255)
                                                                                                                                        cacCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGt   <  1:256179/149‑1 (MQ=255)
                                                                                                                                         acCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGCTCGACAGCGAGCTGGTg  >  1:117779/1‑149 (MQ=255)
                                                                                                                                         acCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTg  <  1:140809/149‑1 (MQ=255)
                                                                                                                                            gTTTCGCCGCTGCGGGATCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACCTTCTGGCGTTGGATATCTCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGCTCGACAGACAGCTGGTg  >  7:143789/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
ATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCAGCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTG  >  NC_000913/2223345‑2223630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑