Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,416,943	C→G	5.3%	intergenic (+52/‑138)	pta → / → yfcC	phosphate acetyltransferase/putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,943	0	C	G	5.3%	260.2 / 5.5	76	intergenic (+52/‑138)	pta/yfcC	phosphate acetyltransferase/putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base C (36/36);  new base G (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

CGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCG  >  NC_000913/2416796‑2417087                                                                                                                                                    |                                                                                                                                                 cgcAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCa                                                                                                                                                 <  8:121727/149‑1 (MQ=255)  gcAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCAc                                                                                                                                                >  8:185774/1‑149 (MQ=255)    aaGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTa                                                                                                                                              >  6:287749/1‑149 (MQ=255)        cGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTc                                                                                                                                          <  6:233989/149‑1 (MQ=255)             aaCGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGtt                                                                                                                                     >  8:54361/1‑149 (MQ=255)                gACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGttttt                                                                                                                                  >  5:14860/1‑149 (MQ=255)                gACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACAATTTCCGGttttt                                                                                                                                  >  8:65938/1‑149 (MQ=255)                  ccTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTa                                                                                                                                >  8:259631/1‑149 (MQ=255)                    tGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTAtt                                                                                                                              >  1:40850/1‑149 (MQ=255)                         cGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTctct                                                                                                                          >  4:3149/1‑148 (MQ=255)                          gTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTa                                                                                                                        <  1:281838/149‑1 (MQ=255)                           tGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTaa                                                                                                                       <  1:289737/149‑1 (MQ=255)                                     gTTGACGATATCGTCTACACCATCGCGCTGACTGCTATTCAGTCTACACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTaa                                                                                                             <  1:249115/149‑1 (MQ=255)                                      ttGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAAt                                                                                                            <  6:14860/149‑1 (MQ=255)                                        gACGATATCGTCTACACCATCGCGCGGACTGCGATGCAGTCTGCACAGCAGCAGTACTCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATc                                                                                                           <  2:280246/148‑1 (MQ=255)                                         aCGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCt                                                                                                         >  4:141557/1‑149 (MQ=255)                                            atatCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTc                                                                                                      <  3:136128/149‑1 (MQ=255)                                               tCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTg                                                                                                    >  7:71293/1‑148 (MQ=255)                                               tCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTg                                                                                                    <  8:71293/148‑1 (MQ=255)                                               tCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGa                                                                                                   <  1:318728/149‑1 (MQ=255)                                                         cATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCtt                                                                                            <  3:275402/146‑1 (MQ=255)                                                         cATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCtt                                                                                            >  4:275402/1‑146 (MQ=255)                                                         cATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCt                                                                                         >  2:162564/1‑149 (MQ=255)                                                         cATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCt                                                                                         >  8:180592/1‑149 (MQ=255)                                                          aTCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTc                                                                                                      >  5:303122/1‑135 (MQ=255)                                                          aTCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTc                                                                                                      <  6:303122/135‑1 (MQ=255)                                                           tCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGGATTAATCCTTTCTGATTATCTTGCtt                                                                                        >  6:279380/1‑148 (MQ=255)                                                             gcgcTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTaa                                                                                      <  2:8715/148‑1 (MQ=255)                                                              cgcTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACt                                                                                    <  7:54361/149‑1 (MQ=255)                                                              cgcTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACt                                                                                    <  6:303279/149‑1 (MQ=255)                                                                  gACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTgcgc                                                                                >  3:206096/1‑149 (MQ=255)                                                                   aCTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCt                                                                               <  3:3149/149‑1 (MQ=255)                                                                     tGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTg                                                                              >  5:97133/1‑148 (MQ=255)                                                                      gCGATTCAGTCTACACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATc                                                                                              <  8:3259/131‑1 (MQ=255)                                                                        gATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCAt                                                                           >  7:286085/1‑148 (MQ=255)                                                                          ttCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCaa                                                                        >  3:258207/1‑149 (MQ=255)                                                                            cAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAAt                                                                       <  6:178833/148‑1 (MQ=255)                                                                              gTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAGTGATTATTTCCGGtt                                                                                                                                     <  8:323600/84‑1 (MQ=38)                                                                              gTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAGTGATTATTTCCGGtt                                                                                                                                     >  7:323600/1‑84 (MQ=38)                                                                                 tGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTg                                                                 >  2:80392/1‑149 (MQ=255)                                                                                              tAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTAAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACtt                                                    >  7:225167/1‑149 (MQ=255)                                                                                                aTCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACttt                                                   <  2:40850/148‑1 (MQ=255)                                                                                                 tctcGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTg                                                  >  1:186273/1‑148 (MQ=255)                                                                                                 tctcGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTg                                                  >  2:94034/1‑148 (MQ=255)                                                                                                   tcGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTTATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATAGCGCCATCACACTTTGCa                                                >  5:168673/1‑148 (MQ=255)                                                                                                     gTCTTCATCCGCAGGTTTGCGCTGCGTATATCTGTACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCAtt                                              <  7:65938/148‑2 (MQ=255)                                                                                                     gTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCa                                                <  8:275463/146‑1 (MQ=255)                                                                                                     gTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCa                                                <  1:80392/146‑1 (MQ=255)                                                                                                     gTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATAc                                             >  6:251013/1‑149 (MQ=255)                                                                                                     gTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATAc                                             <  5:251013/149‑1 (MQ=255)                                                                                                     gTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATAc                                             <  7:259631/149‑1 (MQ=255)                                                                                                       catcatCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACtt                                           <  2:303224/149‑1 (MQ=255)                                                                                                         tcatcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTAc                                         >  2:304218/1‑149 (MQ=255)                                                                                                           atcCGCAGCTTTGCGCTGCTGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATATGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATATCGCCATCCCACTTTGCATACTTAc                                         <  5:249411/147‑1 (MQ=255)                                                                                                              cGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACtt                                    >  5:275208/1‑149 (MQ=255)                                                                                                               gCAGCTTTGCGCTGCGGATATCTGAACCGGAAAGAATCACTATTTCCGCTACTATATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACttt                                   >  2:209837/1‑149 (MQ=255)                                                                                                                   cTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACtttgttt                               >  2:69239/1‑149 (MQ=255)                                                                                                                        cgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTtgtg                           <  5:270793/148‑1 (MQ=255)                                                                                                                        cgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTtgtg                           <  8:286085/148‑1 (MQ=255)                                                                                                                        cgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGc                          <  1:304218/149‑1 (MQ=255)                                                                                                                          cTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCaa                        <  7:287565/149‑1 (MQ=255)                                                                                                                             cGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCttt                                                                                                       <  4:298214/67‑1 (MQ=255)                                                                                                                             cGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCttt                                                                                                       >  3:298214/1‑67 (MQ=255)                                                                                                                             cGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAgg                      <  2:186273/148‑1 (MQ=255)                                                                                                                               gATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGaa                   >  8:50662/1‑149 (MQ=255)                                                                                                                                atatCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAat                  <  4:206096/149‑1 (MQ=255)                                                                                                                                     tGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTg             >  8:132061/1‑149 (MQ=255)                                                                                                                                     tGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTg             >  8:1543/1‑149 (MQ=255)                                                                                                                                      gAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGAGTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCCCTTTGTTTTGTGCAAGGGAATATTTgc            >  5:290963/1‑149 (MQ=255)                                                                                                                                         ccGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTgcgc          >  3:58733/1‑148 (MQ=255)                                                                                                                                          cGGAAATAGTGATTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATAc                                             <  2:278537/112‑1 (MQ=255)                                                                                                                                          cGGAAATAGTGATTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATAc                                             >  1:278537/1‑112 (MQ=255)                                                                                                                                          cGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTa        <  6:228349/149‑1 (MQ=255)                                                                                                                                           ggAAATACTCACTATTTCCTGTATTTTATTCTCTTAATATGCATTATTGCTTTGTGATTATCTTGCTTAACTGCGCTGCATGAATGTATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGGAAGGGAATATTTGCGCTAt       <  7:326841/149‑1 (MQ=255)                                                                                                                                                taatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGTAATATTTGCGCTATGTCCg  <  1:69239/149‑1 (MQ=255)                                                                                                                                                taatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGAATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCTCTATGTCCg  >  1:316457/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCG  >  NC_000913/2416796‑2417087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑