New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 232564	54 (1.450)	8 (0.240)	8/226	2.9	13.8%	intergenic (+15/+33)	yafE/mltD	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
	?	NC_000913	= 232571	51 (1.510)					intergenic (+22/+26)	yafE/mltD	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Frequency below/above cutoff threshold.

TTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGT  >  NC_000913/232437‑232711                                                                                                                                       |                                                                                                                                             ttCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTtta                                                                                                                                 <  1:184169/148‑1 (MQ=255)    gCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTtattat                                                                                                                             >  5:76500/1‑149 (MQ=255)    gCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTtattat                                                                                                                             >  4:133670/1‑149 (MQ=255)           ccAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                                      <  6:76500/149‑1 (MQ=255)               cAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCa                                                                                                                  <  3:133670/149‑1 (MQ=255)                 gagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGATTCCCCcggtgccttttttatttatgccgctttatg                                                                                                                <  1:165180‑M2/149‑31 (MQ=255)                            cAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGATTCCCCcggtgccttttttatttatgccgctttatgtgcatctacca                                                                                                     >  2:165180‑M2/1‑108 (MQ=255)                                   gagaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          >  2:5723/1‑137 (MQ=255)                                   gagaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          <  1:5723/137‑1 (MQ=255)                                                   ttGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGc                                                                                                        >  3:68602/1‑123 (MQ=255)                                                   ttGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGc                                                                                                        <  4:68602/123‑1 (MQ=255)                                                   ttGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCAACGGGGTAATAGGTGCATTTTTATTATCTGGTTTTTCAGGAATATGGCATGTTGTTGTTTTTCAAAAACAACg                                                                               >  8:49983/1‑148 (MQ=255)                                                     gCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          <  1:72436/119‑1 (MQ=255)                                                     gCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          >  2:72436/1‑119 (MQ=255)                                                       aGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGc                                                                          <  1:162261/149‑1 (MQ=255)                                                        gAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAga                                                                          >  3:163636/1‑147 (MQ=255)                                                          atgatgGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTa                                                                       >  2:48355/1‑149 (MQ=255)                                                                gCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGcc                                                                  <  1:48355/148‑1 (MQ=255)                                                                 cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                >  7:50743/1‑117 (MQ=255)                                                                 cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                <  8:50743/117‑1 (MQ=255)                                                                 cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                                >  1:147840/1‑149 (MQ=255)                                                                        aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGTCACCGGGGGACTCGGTGCCTTTTTATTATCTGGTTTGTCAGTAATCTGGCATGTGGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCa                                                          <  7:49983/148‑1 (MQ=255)                                                                           aGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGAtt                                                      >  6:73216/1‑149 (MQ=255)                                                                             tGATATCATCATGGTAGATGCTCATAAATAGGGATAAATAAATAAGGCACCGGGGGAATCGGTTCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                                <  2:100357/137‑1 (MQ=255)                                                                             tGATATCATCATGGTAGATGCACATAAAGAGGCATAAATAAAAAAGGCACAGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCCGCTTATCGCCTg                                                                >  1:100357/1‑137 (MQ=255)                                                                               atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttg                                                                                                  >  6:14058/1‑101 (MQ=255)                                                                               atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttg                                                                                                  <  5:14058/101‑1 (MQ=255)                                                                               atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCg                                                    >  7:32766/1‑147 (MQ=255)                                                                                 atcatcATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                >  8:78331/1‑149 (MQ=255)                                                                                   catcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgt                                                                                                    <  3:45948/95‑1 (MQ=255)                                                                                   catcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgt                                                                                                    >  4:45948/1‑95 (MQ=255)                                                                                    atcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAAt                                                                                                                                              >  3:8844/1‑52 (MQ=255)                                                                                    atcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAAt                                                                                                                                              <  4:8844/52‑1 (MQ=255)                                                                                           tAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaaca                                                                                  >  3:41479/1‑105 (MQ=255)                                                                                           tAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaaca                                                                                  <  4:41479/105‑1 (MQ=255)                                                                                             gATGCACATAAAGCGGCATAAATAAAAAATGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCa                                    <  4:163636/149‑1 (MQ=255)                                                                                               tGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAgc                                  <  8:32766/149‑1 (MQ=255)                                                                                                    aTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCAt                              >  1:13975/1‑148 (MQ=255)                                                                                                      aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCa                                                  <  8:98120/126‑1 (MQ=255)                                                                                                      aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCa                                                  >  7:98120/1‑126 (MQ=255)                                                                                                      aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                <  1:117682/128‑1 (MQ=255)                                                                                                      aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                >  2:117682/1‑128 (MQ=255)                                                                                                              ataaataaAAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgc                                                                  <  5:99998‑M2/102‑77 (MQ=255)                                                                                                              ataaataaAAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgc                                                                  >  6:99998‑M2/1‑26 (MQ=255)                                                                                                                aaataaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatc                                                                         <  4:161006‑M2/93‑70 (MQ=255)                                                                                                                aaataaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatc                                                                         >  3:161006‑M2/1‑24 (MQ=255)                                                                                                                   taaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgcaa                                                                <  4:12568‑M2/99‑79 (MQ=255)                                                                                                                   taaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgcaa                                                                >  3:12568‑M2/1‑21 (MQ=255)                                                                                                                      aaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTc                                      <  6:100808/122‑1 (MQ=255)                                                                                                                      aaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTc                                      >  5:100808/1‑122 (MQ=255)                                                                                                                        aaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGcc         >  3:121843/1‑149 (MQ=255)                                                                                                                          ggCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGt       <  5:73216/149‑1 (MQ=255)                                                                                                                             aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATc                             >  1:117006/1‑124 (MQ=255)                                                                                                                             aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATc                             <  2:117006/124‑1 (MQ=255)                                                                                                                             aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                      <  7:33901/131‑1 (MQ=255)                                                                                                                             aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                      >  8:33901/1‑131 (MQ=255)                                                                                                                             aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGc    <  2:147840/149‑1 (MQ=255)                                                                                                                              ccGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCg   >  2:137328/1‑149 (MQ=255)                                                                                                                               cGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTGGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGt  <  7:78331/149‑1 (MQ=255)                                                                                                                                 ggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCAc            >  6:95435/1‑137 (MQ=255)                                                                                                                                 ggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCAc            <  5:95435/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                             TTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGT  >  NC_000913/232437‑232711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.