New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	781378 =	40 (1.100)	4 (0.150) +AGTTGACTTTTAATCAATTGGTCGCAGGTTCGA	4/184	3.3	13.1%	noncoding (10/76 nt)	lysZ	tRNA‑Lys
	?	NC_000913	781791 =	32 (0.880)					intergenic (+139/‑294)	lysQ/nadA	tRNA‑Lys/quinolinate synthase, subunit A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCA  >  NC_000913/781230‑781526                                                                                                                                                     |                                                                                                                                                     cATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg                                                                                                                                                      >  4:82984/1‑149 (MQ=255)           aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                                                                                                                                             <  3:82984/148‑1 (MQ=255)                         aaGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                                                                                                                               <  8:30952/148‑1 (MQ=255)                                       gcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc                                                                                                                >  5:106119/1‑149 (MQ=255)                                              aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                                         <  6:106119/149‑1 (MQ=255)                                                     gTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAACTCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTAGGTAGAGCAGTGGACTTTTAATCAATTGCTCGCAGGTTCGAATcc                                                                                                  >  7:39494/1‑149 (MQ=255)                                                                 aagcgaGTCATCCTGCACGACCCACCAATATTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTATAATCAATTGGTCGCAGGATCGATTCCTGCACGACccac                                                                                      >  2:135764/1‑149 (MQ=255)                                                                           tCCTGCACGACCCACCAATATTAAAGATTTGACCGGAGTAAAAATCTTTCAGGTAACACCCGTATGGTAGGTTAGCACAGTTGGTAGAGAAGTTGAGTTTTAATCTATTGGTCGAAGGTTCGAAGCCTGCACGAAAAACAAATTTAAAg                                                                            >  4:115773/1‑149 (MQ=21)                                                                              tGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                                         >  4:141798/1‑101 (MQ=255)                                                                              tGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                                         <  3:141798/101‑1 (MQ=255)                                                                               gCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                                                                      >  3:113576/1‑87 (MQ=255)                                                                               gCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                                                                      <  4:113576/87‑1 (MQ=255)                                                                               gCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAggtgg                                                                        >  1:114562/1‑149 (MQ=255)                                                                                         ccaATCTTTAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTgg                                                                 >  6:109223/1‑146 (MQ=255)                                                                                          caATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                                                                          <  3:57741/88‑1 (MQ=255)                                                                                          caATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                                                                          >  4:57741/1‑88 (MQ=255)                                                                                                tAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAggtggt                                                                       <  2:178978/133‑1 (MQ=255)                                                                                                tAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAggtggt                                                                       >  1:178978/1‑133 (MQ=255)                                                                                                    gATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAg                                                   <  5:109223/149‑1 (MQ=255)                                                                                                            ccGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCa                                                                                              <  6:144580/98‑1 (MQ=255)                                                                                                            ccGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCa                                                                                              >  5:144580/1‑98 (MQ=255)                                                                                                                 tAAAAATCTTTCAGTTAACAACCGTATGGGGCGTTAGCTCAGTTGGTAGAGCAGATGACTTTTAATCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGt                                      <  3:115773/149‑1 (MQ=25)                                                                                                                     aaTCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAg                                   >  4:58198/1‑148 (MQ=255)                                                                                                                      aTCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                                                                                               >  2:185086/1‑87 (MQ=255)                                                                                                                      aTCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                                                                                               <  1:185086/87‑1 (MQ=255)                                                                                                                           tCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACgg                             <  3:58198/148‑1 (MQ=255)                                                                                                                                tAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGaa                       <  1:135764/149‑1 (MQ=255)                                                                                                                                    acCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGtt                                          <  5:35521/126‑1 (MQ=255)                                                                                                                                    acCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGtt                                          >  6:35521/1‑126 (MQ=255)                                                                                                                                      ccGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTATAGGTGGTTAc                                                                    <  5:60962/98‑1 (MQ=255)                                                                                                                                      ccGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTAc                                                                    >  6:60962/1‑98 (MQ=255)                                                                                                                                      ccGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGaa                                                    >  6:111961/1‑114 (MQ=255)                                                                                                                                      ccGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGaa                                                    <  5:111961/114‑1 (MQ=255)                                                                                                                                             ggTCGTTAGCTCAGTTTGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTa                                    >  6:2291/1‑123 (MQ=255)                                                                                                                                             ggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTa                                    <  5:2291/123‑1 (MQ=255)                                                                                                                                              gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGc                   >  2:73212/1‑139 (MQ=255)                                                                                                                                              gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGc                   <  1:73212/139‑1 (MQ=255)                                                                                                                                              gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGt         >  5:71818/1‑149 (MQ=255)                                                                                                                                                cGTTAGCTCAGTTGGTCGAGCAGTTGACTTTTACTCTAGTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTc        <  8:39494/148‑1 (MQ=18)                                                                                                                                                 gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTc        >  8:80256/1‑147 (MQ=255)                                                                                                                                                     gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCa  >  8:95275/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCA  >  NC_000913/781230‑781526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.