New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4349222 =125 (1.410)4 (0.050) 4/242 NT 3.6% intergenic (‑478/+93) dcuB/dcuR C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor/response regulator in two‑component regulatory system with DcuS
?NC_000913 4349263 = 101 (1.250)intergenic (‑519/+52) dcuB/dcuR C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor/response regulator in two‑component regulatory system with DcuS
Rejected: Frequency below/above cutoff threshold.

CGTCCAGTTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGCCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4349380‑4349222
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aacttgtgccagGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAA  >  NC_000913/4349263‑4349410
                                                                                                                                                                                                                                                                                                                   
CGTCCAGTTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAA                                                                                                                                                                <  1:73893/149‑1
  TCCAGTTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACT                                                                                                                                                              >  1:4083/1‑149
     AGTTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGT                                                                                                                                                           <  7:8340/149‑1
       TTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGC                                                                                                                                                         <  8:7975/149‑1
          ACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGC                                                                                                                                                         >  6:308472/1‑146
           CCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGC                                                                                                                                                         >  3:329260/1‑145
            CGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGC                                                                                                                                                         >  7:23422/1‑144
                                       TCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGC                                                                                                                                                         >  1:276832/1‑117
                                       TCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGC                                                                                                                                                         <  2:276832/117‑1
                                                                                                                    ggCTGGCACAAGTTACATCCTTCATCAGTAAATCTTGTGCCAGGGGTATTTTGCATTACGGTAATTATTATTACTTACAGATAACCAGCGATCCGTTATTGG                                                                                           <  5:260571/100‑1
                                                                                                                    CCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGCCAGGGGAATTTTGCATTACGGAAAAAATAATTACTAACAGATAACCAGCGATCCGTTATTGt                                                                                           >  6:260571/1‑101
                                                                                                                          CACAAGTTACATCCTTCATCAGTAAAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGT                                                                  >  1:198771/1‑121
                                                                                                                          CACAAGTTACATCCTTCATCAGTAAAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGT                                                                  <  2:198771/121‑1
                                                                                                                                                    ACTTGTGCCAGGGGTATTTTGCATTACGGTAATTATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGA            <  5:342106/149‑1
                                                                                                                                                     CTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGA            >  3:319580/1‑148
                                                                                                                                                      TTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGA            <  4:311319/147‑1
                                                                                                                                                      TTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGA            >  3:311319/1‑147
                                                                                                                                                        GTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATA          <  4:9036/147‑1
                                                                                                                                                           CCAGGGGTATTTTGCATTACGGTAATAATTATTTCTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGT     <  2:136348/149‑1
                                                                                                                                                           CCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGT     <  2:250767/149‑1
                                                                                                                                                           CCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGG      <  5:135037/148‑1
                                                                                                                                                            CAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGT     >  2:232770/1‑148
                                                                                                                                                             AGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGA   <  6:65734/149‑1
                                                                                                                                                             AGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGA   <  7:64287/149‑1
                                                                                                                                                             AGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTG    >  5:65734/1‑148
                                                                                                                                                             AGGGGTATTTTGCATAACGGTAATAATTATTACTTACACATAACCAGCGAACCGATATTGGCAATATTGTATCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGAAGCCATAATGGATACAGGTG    >  2:333994/1‑148
                                                                                                                                                              GGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAA  >  4:302160/1‑149
                                                                                                                                                              GGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAA  <  6:256438/149‑1
                                                                                                                                                              GGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAA  >  2:227564/1‑149
                                                                                                                                                              GGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAA  <  8:184287/149‑1
                                                                                                                                                                                                                                                                                                                   
CGTCCAGTTTACCGTTACCGTATTCAGGCAGAACACTACTCACTACTGAAACAATATTGCCAATAACGGATCGCTGGTTATCTGTAAGTAATAATTATTACCGTAATGCAAAATACCCCTGGCACAAGTTACATCCTTCATCAGTAAAACTTGTGCCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4349380‑4349222
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aacttgtgccagGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAA  >  NC_000913/4349263‑4349410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.