Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 927,369 C→A 5.4% E22* (GAG→TAG)  aat ← leucyl/phenylalanyl‑tRNA‑protein transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913927,3690CA 5.4% 298.3 / 3.7 87E22* (GAG→TAG) aatleucyl/phenylalanyl‑tRNA‑protein transferase
Reads supporting (aligned to +/- strand):  ref base C (43/39);  new base A (2/3);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGC  >  NC_000913/927223‑927515
                                                                                                                                                  |                                                                                                                                                  
ttCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCAc                                                                                                                                                  >  1:225883/1‑149 (MQ=255)
ttCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCAc                                                                                                                                                  <  6:39425/149‑1 (MQ=255)
     gCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTaa                                                                                                                                              <  8:112366/148‑1 (MQ=255)
     gCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAAt                                                                                                                                             >  6:303395/1‑149 (MQ=255)
      ccATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATg                                                                                                                                            <  8:206771/149‑1 (MQ=255)
       cATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATgc                                                                                                                                           <  2:67490/149‑1 (MQ=255)
       cATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGCCTCACGTAATgc                                                                                                                                           >  3:217805/1‑149 (MQ=255)
         tAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATgcgc                                                                                                                                         >  1:301713/1‑149 (MQ=255)
              ccGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTcc                                                                                                                                    <  8:210405/149‑1 (MQ=255)
                 cgcgGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCggg                                                                                                                                 <  4:191515/149‑1 (MQ=255)
                     gggATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGgggaag                                                                                                                             >  3:163346/1‑149 (MQ=255)
                     gggATAGGGCGACCACCAGAGGATGGGGGCGCCTGGAGAAAACCAAGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGgggaag                                                                                                                             <  8:398626/149‑1 (MQ=255)
                         tCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGgggaagggaa                                                                                                                         <  2:301713/149‑1 (MQ=255)
                                accaccAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGTCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTAtt                                                                                                                  <  5:309049/149‑1 (MQ=255)
                                accaccAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCAATAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTAtt                                                                                                                  <  6:216450/149‑1 (MQ=255)
                                 ccaccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTAtt                                                                                                                  >  3:140529/1‑148 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAATCCATTTACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGACGTTAGGCTCAAGTAATGCGCCTTCAGGTGAAGGGAAGGCTATTGAAt                                                                                                              >  6:292491/1‑149 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGgggaa                                                                                                                              <  7:112827/133‑1 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGgggaa                                                                                                                              >  8:112827/1‑133 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAAt                                                                                                              >  7:148072/1‑149 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAAt                                                                                                              >  1:181948/1‑149 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTAACGTAATGCGCCTTCCGgggaa                                                                                                                              <  2:350361/133‑1 (MQ=255)
                                    ccaGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTAACGTAATGCGCCTTCCGgggaa                                                                                                                              >  1:350361/1‑133 (MQ=255)
                                       gagGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGc                                                                                                           <  4:163346/149‑1 (MQ=255)
                                       gagGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGc                                                                                                           <  6:392155/149‑1 (MQ=255)
                                               ggTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGc                                                                                                   <  5:284660/149‑1 (MQ=255)
                                                gTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCt                                                                                                  >  6:289356/1‑149 (MQ=255)
                                                gTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCt                                                                                                  >  6:52303/1‑149 (MQ=255)
                                                   gCCTGGAGAAAACCACGGAAAAATACCACTCTGGTAAGCCATTAACAGGCGCGCAGGGATAAGATCGCCCCCAAGTGCCAGCATGCCGTTAGGCTCACGTAATGCTCCTTCCGGGGAAGGGAAGGCTATTGAATGGAGAGAAAGCTgca                                                                                               >  4:312313/1‑147 (MQ=255)
                                                   gCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGaa                                                                                               >  5:359102/1‑149 (MQ=255)
                                                    ccTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAAc                                                                                              <  3:120241/149‑1 (MQ=255)
                                                     cTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCg                                                                                                          >  3:90614/1‑136 (MQ=255)
                                                     cTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCg                                                                                                          <  4:90614/136‑1 (MQ=255)
                                                     cTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAAc                                                                                              >  4:150275/1‑148 (MQ=255)
                                                     cTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAAc                                                                                              >  7:83908/1‑148 (MQ=255)
                                                      tGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAAc                                                                                              >  2:296804/1‑147 (MQ=255)
                                                                cACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGAc                                                                                  >  2:282910/1‑149 (MQ=255)
                                                                cACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTAACGTAATGCGCATTCCGGGGAAGGGAAGGCTATTGAATGGAGAGAACGCTGAACAAGGCGAATGAc                                                                                  >  4:149872/1‑149 (MQ=255)
                                                                    gAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGc                                                                               >  4:18391/1‑148 (MQ=255)
                                                                    gAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCa                                                                              <  1:296804/149‑1 (MQ=255)
                                                                            ccACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCa                                                                      <  6:212085/149‑1 (MQ=255)
                                                                            ccACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCa                                                                      <  5:113737/149‑1 (MQ=255)
                                                                              aCGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACg                                                                    >  5:109506/1‑149 (MQ=255)
                                                                                  tGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAg                                                                >  8:57120/1‑149 (MQ=255)
                                                                                    gTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATgg                                                                                                            >  4:306153/1‑103 (MQ=255)
                                                                                    gTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATgg                                                                                                            <  3:306153/103‑1 (MQ=255)
                                                                                    gTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCATCCGGGGAAGGGAAGGCTATAGAATTGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTc                                                              >  2:103558/1‑149 (MQ=255)
                                                                                         ccATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATc                                                         <  1:319762/149‑1 (MQ=255)
                                                                                          cATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCaaaa                                                                           <  4:341966/130‑1 (MQ=255)
                                                                                          cATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCaaaa                                                                           >  3:341966/1‑130 (MQ=255)
                                                                                            ttAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGtt                                                      >  8:52912/1‑149 (MQ=255)
                                                                                            ttAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGtt                                                      >  3:89215/1‑149 (MQ=255)
                                                                                            ttAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGtt                                                      <  2:225883/149‑1 (MQ=255)
                                                                                                  ggCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCaataa                                                >  7:16384/1‑149 (MQ=255)
                                                                                                  ggCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCaataa                                                >  3:317365/1‑149 (MQ=255)
                                                                                                   gcgcgcAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCaataat                                               <  3:158965/149‑1 (MQ=255)
                                                                                                   gcgcgcAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCAATTGAATGGCGAGAAAGCTGACCCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCaataa                                                >  4:303867/1‑148 (MQ=255)
                                                                                                   gcgcgcAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGATAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGACCCAGGCGCATGACCGAAAACCTCCACGCAAGTCGGCTCGTTCaataa                                                >  2:269929/1‑148 (MQ=255)
                                                                                                    cgcgcAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAataata                                              <  6:359102/149‑1 (MQ=255)
                                                                                                    cgcgcAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAataata                                              <  3:150892/149‑1 (MQ=255)
                                                                                                      cgcATGGCTAAGATAGCCCCCAAGTTCCATCAGGCCTGTAGGCTAACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGGAAAACTCCACGCAAGTCGGATCGTTCAATAATAGc                                            <  3:149872/149‑1 (MQ=255)
                                                                                                      cgcAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGc                                            <  1:282910/149‑1 (MQ=255)
                                                                                                         atggCCAAGATCGGCCCCAAGGGCCAGCAGGCGGTTAGGCTCAAGTAATGCGCCGTCCGGGGGAGGGAAGGCTATTGTATGGCGAGAATGCTGAAACCGGCGCATGACCGCAAAACTCCACTCAAGTGGGATCGTTGAATTATACCttt                                         <  4:338213/147‑2 (MQ=255)
                                                                                                         aGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTa                                         >  2:146398/1‑149 (MQ=255)
                                                                                                                aGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAAccc                                  >  1:359474/1‑149 (MQ=255)
                                                                                                                 gATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCt                                 <  1:146398/149‑1 (MQ=255)
                                                                                                                 gATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCt                                 <  5:303395/149‑1 (MQ=255)
                                                                                                                 gATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTAATGAATGGCGAGAAAGCTGAACAAGGCGCATGACCGCAAAACACCACGCAAGTCGGACCGTTCAATAATAGCTTACAAACCCt                                 >  2:263613/1‑149 (MQ=255)
                                                                                                                   tCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGc                               <  7:57120/149‑1 (MQ=255)
                                                                                                                     gCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCtt                             <  2:181948/149‑1 (MQ=255)
                                                                                                                      cccccAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCCAACCTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAACCCCTGCtt                             >  7:360762/1‑148 (MQ=255)
                                                                                                                          cAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACt                        >  5:253178/1‑149 (MQ=255)
                                                                                                                           aaGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTg                       <  6:377634/149‑1 (MQ=255)
                                                                                                                            aGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTgg                      <  7:52912/149‑1 (MQ=255)
                                                                                                                             gTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTgg                      >  7:226920/1‑148 (MQ=255)
                                                                                                                                cgatcagGCCGTTAGGCTAACGTAATGCTCCTTCCGGGGAAGGTAAGGATATTGAATGGCGAGAAAGCTTAACAAGGCGCATGACAGCAAAACTCCACGCAATTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGtaa                   <  3:312313/144‑1 (MQ=255)
                                                                                                                                ccAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGtaa                   >  7:52267/1‑148 (MQ=255)
                                                                                                                                    cagGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACg              >  1:10128/1‑149 (MQ=255)
                                                                                                                                       gCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCaaaa                                                                           >  6:46359/1‑85 (MQ=255)
                                                                                                                                       gCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCaaaa                                                                           <  5:46359/85‑1 (MQ=255)
                                                                                                                                       gCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGccc           <  8:16384/149‑1 (MQ=255)
                                                                                                                                         cGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCt         <  5:289356/149‑1 (MQ=255)
                                                                                                                                          gTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTg        >  3:38555/1‑149 (MQ=255)
                                                                                                                                             aGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTc      <  5:52303/148‑1 (MQ=255)
                                                                                                                                                cTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGGATGCCCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGc  <  5:292491/149‑1 (MQ=255)
                                                                                                                                                 tCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGc  >  2:166906/1‑148 (MQ=255)
                                                                                                                                                 tCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTAGAACTGGTAATAACGCCCCTGTCTGGc  >  3:221674/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCTAAGATCGCCCCCAAGTGCCAGCAGGCCGTTAGGCTCACGTAATGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGC  >  NC_000913/927223‑927515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: