Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,174,304 C→A 6.4% V288F (GTT→TTT)  ycfT ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,174,3040CA 6.4% 233.4 / 4.0 71V288F (GTT→TTT) ycfTinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base A (3/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

CGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATA  >  NC_000913/1174156‑1174452
                                                                                                                                                    |                                                                                                                                                    
cGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAAc                                                                                                                                                      <  5:26120/149‑1 (MQ=255)
     tgAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGAGCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGaa                                                                                                                                                 >  2:210637/1‑149 (MQ=255)
      gAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAAt                                                                                                                                                >  5:320799/1‑149 (MQ=255)
       aaaGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAAt                                                                                                                                                <  3:159388/148‑1 (MQ=255)
       aaaGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATg                                                                                                                                               >  6:213325/1‑149 (MQ=255)
       aaaGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATg                                                                                                                                               >  3:325292/1‑149 (MQ=255)
       aaaGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATg                                                                                                                                               <  2:304221/149‑1 (MQ=255)
                 tACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGggtgg                                                                                                                                     >  4:362916/1‑149 (MQ=255)
                    cAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGgtgt                                                                                                                                  <  5:315141/149‑1 (MQ=255)
                    aaGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTATACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGAAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGgtgt                                                                                                                                  <  1:32123/148‑1 (MQ=255)
                             cGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCaa                                                                                                                         <  4:312685/149‑1 (MQ=255)
                             cGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCaa                                                                                                                         >  2:63796/1‑149 (MQ=255)
                               cAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATg                                                                                                                       <  8:197884/149‑1 (MQ=255)
                                 aCAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGgt                                                                                                                     <  7:323451/149‑1 (MQ=255)
                                     tACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTg                                                                                                                 <  4:283561/149‑1 (MQ=255)
                                                 aaCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGc                                                                                                                                              <  3:163128/108‑1 (MQ=255)
                                                 aaCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAAt                                                                                                                        <  4:173969/130‑1 (MQ=255)
                                                 aaCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAAt                                                                                                                        >  3:173969/1‑130 (MQ=255)
                                                 aaCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATcaca                                                                                                     <  3:258706/149‑1 (MQ=255)
                                                 aaCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATcaca                                                                                                     <  7:384982/149‑1 (MQ=255)
                                                 aaCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAAACAGAATGc                                                                                                                                              >  4:163128/1‑108 (MQ=255)
                                                  aCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAAACAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACAt                                                                                                    >  2:82303/1‑149 (MQ=255)
                                                  aCTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAAACAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACAt                                                                                                    >  2:87638/1‑149 (MQ=255)
                                                       cAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTcagc                                                                                               <  3:362916/149‑1 (MQ=255)
                                                       cAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATCCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTcagc                                                                                               >  1:363468/1‑149 (MQ=255)
                                                        aaaGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTcag                                                                                                >  2:31569/1‑147 (MQ=255)
                                                         aaGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTcagcag                                                                                             >  7:45766/1‑149 (MQ=255)
                                                          aGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAgg                                                                                            >  3:162778/1‑149 (MQ=255)
                                                           gggATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGAGCAGCGTGCTGCGTTCATTTGCGCTAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGGAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGc                                                                                           <  1:210637/149‑1 (MQ=255)
                                                             gATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCtg                                                                                         <  3:105558/149‑1 (MQ=255)
                                                             gATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCtg                                                                                         <  1:31569/149‑1 (MQ=255)
                                                             gATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTCTAGGAGACAATAACATTAAGCAGGCtg                                                                                         >  2:80749/1‑149 (MQ=255)
                                                                  aCCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCtggt                                                                                       >  8:111781/1‑146 (MQ=255)
                                                                  aCCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCtggt                                                                                       <  7:111781/146‑1 (MQ=255)
                                                                    cagcagGAGTGTCAGTTCGACTTGCGGCGACCAGAGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGGGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAgc                                                                                  <  1:72951/149‑1 (MQ=255)
                                                                      gcagGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAgcg                                                                                 >  7:364724/1‑148 (MQ=255)
                                                                       cagGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCa                                                                               >  5:158726/1‑149 (MQ=255)
                                                                         ggAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATa                                                                             >  2:307365/1‑149 (MQ=255)
                                                                          gAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATAc                                                                            <  4:325292/149‑1 (MQ=255)
                                                                                  gTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAAACAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGt                                                                    <  1:87638/149‑1 (MQ=255)
                                                                                  gTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAAACAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGt                                                                    <  1:82303/149‑1 (MQ=255)
                                                                                     cGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTg                                                                  <  8:376987/148‑1 (MQ=255)
                                                                                           gcggcgACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTa                                                            <  1:63796/148‑1 (MQ=255)
                                                                                           gcggcgACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTa                                                            <  1:308544/148‑1 (MQ=255)
                                                                                                   cAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTc                                                               <  4:311800/137‑1 (MQ=255)
                                                                                                   cAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTc                                                               >  3:311800/1‑137 (MQ=255)
                                                                                                      cGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTAt                                                           <  2:260561/138‑1 (MQ=255)
                                                                                                      cGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTAt                                                           >  1:260561/1‑138 (MQ=255)
                                                                                                           tgcGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCa                                           >  7:120311/1‑149 (MQ=255)
                                                                                                           tgcGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCa                                           >  1:210375/1‑149 (MQ=255)
                                                                                                            gcGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCAt                                          >  8:296850/1‑149 (MQ=255)
                                                                                                              gTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGa                                        <  5:213325/149‑1 (MQ=255)
                                                                                                                  aTTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcac                                    >  6:191995/1‑149 (MQ=255)
                                                                                                                   tttGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcac                                    >  8:72617/1‑148 (MQ=255)
                                                                                                                   tttGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcac                                    >  1:149517/1‑148 (MQ=255)
                                                                                                                        gcAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTCTAGGAAACAATAACAATAAGCAGGCTGGTGGAGCGCATACCGAATCGTTGGTCGTTTTGATAATACAGCTTCATGATCACCACAAt                              >  8:388365/1‑149 (MQ=255)
                                                                                                                          aaGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATATCAATGGTCTAGGAAACAATAAAAATAAGAAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACCGCTTCATGGTcacaaacatc                             >  6:1296/1‑141 (MQ=255)
                                                                                                                            gCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTg                                                                  <  4:213931/109‑1 (MQ=255)
                                                                                                                            gCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTg                                                                  >  3:213931/1‑109 (MQ=255)
                                                                                                                            gCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATATAACa                                                 <  4:240381/126‑1 (MQ=255)
                                                                                                                            gCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAGAACa                                                 >  3:240381/1‑126 (MQ=255)
                                                                                                                              agagTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATAc                        <  8:120311/149‑1 (MQ=255)
                                                                                                                               gagTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATAcc                       <  8:364724/149‑1 (MQ=255)
                                                                                                                                 gTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCa                      <  5:191995/148‑1 (MQ=255)
                                                                                                                                     aGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGa                  <  6:320799/148‑1 (MQ=255)
                                                                                                                                        tGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGacaa               >  3:122694/1‑148 (MQ=255)
                                                                                                                                          aaTATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATc                                                                                                        <  7:395060/57‑1 (MQ=255)
                                                                                                                                          aaTATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATc                                                                                                        >  8:395060/1‑57 (MQ=255)
                                                                                                                                          aaTATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTCTTCGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAAcag            >  4:94798/1‑149 (MQ=255)
                                                                                                                                            tatTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAAcagca          <  2:210375/149‑1 (MQ=255)
                                                                                                                                                  aaCCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACagcagc        >  1:9521/1‑145 (MQ=255)
                                                                                                                                                   aCCAGAATGCGATGGGTGGTGTAGATAGCAATGGTCTTGGAGACAATAAAATTAAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCgaga    >  2:90736/1‑148 (MQ=255)
                                                                                                                                                    ccAGAATGCGATGGGTGTGGTAGATAGCAATGGTGTTGTAGCCAAACCCATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCCGCGACAACAGCAGCGAGATa  <  2:330214/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGCTGTGAAAGTTTTCTTACCAGCAAGCCCGCAACAGTACAGATGAACAAACTGACAAAGGGATAAACCAGCAGGAGTGTCAGTTCGACTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATA  >  NC_000913/1174156‑1174452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑