Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,217,116 T→G 5.8% intergenic (+120/‑212) ymgC → / → ycgG Blue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,217,1160TG 5.8% 321.0 / 6.9 110intergenic (+120/‑212)ymgC/ycgGBlue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (54/49);  new base G (2/5);  total (56/54)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGCTCTCTG  >  NC_000913/1216968‑1217262
                                                                                                                                                    |                                                                                                                                                  
ttaATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGtt                                                                                                                                                    >  8:102486/1‑149 (MQ=255)
   aTGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc                                                                                                                                                 >  4:25960/1‑149 (MQ=255)
   aTGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGCGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtct                                                                                                                                                  <  4:108243/148‑1 (MQ=255)
        aGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTATAGctct                                                                                                                                            >  5:199649/1‑149 (MQ=255)
          ggAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc                                                                                                                                           >  6:74789/1‑148 (MQ=255)
          ggAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc                                                                                                                                           >  5:236114/1‑148 (MQ=255)
           gAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCa                                                                                                                                         >  2:146797/1‑149 (MQ=255)
               ccGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCt                                                                                                                                     >  7:161675/1‑149 (MQ=255)
                  tGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTTAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGa                                                                                                                                  >  2:6865/1‑149 (MQ=255)
                   gCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGCGTCAAGAATGGAGAGCGAGAACTCTCCATTCTTGa                                                                                                                                  >  4:96842/1‑148 (MQ=38)
                           aGAACTTGAGTAAGGTTTTAGATTAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTg                                                                                                                            <  2:58911/146‑1 (MQ=255)
                              aCTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCGCCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATcc                                                                                                                      >  8:30983/1‑149 (MQ=255)
                               cTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATccc                                                                                                                     >  2:166636/1‑149 (MQ=255)
                               cTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCCCGCTCTCCATTCTTGACGCCTGATATccc                                                                                                                     <  8:31424/149‑1 (MQ=255)
                                ttGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCg                                                                                                                    >  1:320840/1‑149 (MQ=255)
                                   aGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGcc                                                                                                                  <  8:160264/148‑1 (MQ=255)
                                   aGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGCGAGAACTCTCCATTCTTGACACCTGatattgcgga                                                                                                                  <  2:87860/148‑7 (MQ=25)
                                      aaGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAAc                                                                                                              >  7:127232/1‑149 (MQ=255)
                                         gTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGTAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTa                                                                                                           >  3:21424/1‑149 (MQ=255)
                                         gTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTa                                                                                                           >  4:144998/1‑149 (MQ=255)
                                         gTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATACCAGGTGTCAAGAATGGAGAGTTCTCGctc                                                                                                                                             <  1:285587/115‑1 (MQ=255)
                                         gTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATACCAGGTGTCAAGAATGGAGAGTTCTCGctc                                                                                                                                             >  2:285587/1‑115 (MQ=255)
                                          ttttAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTAt                                                                                                          >  1:316419/1‑149 (MQ=255)
                                             tAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTAttt                                                                                                        <  7:204471/148‑1 (MQ=255)
                                              aGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTAtttt                                                                                                       <  2:54146/148‑1 (MQ=255)
                                               gAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGt                                                                                                     <  6:39561/149‑1 (MQ=255)
                                               gAATAAACATCAGCTGTATCACCATGCTGATGCAAAGAGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGCCGGCTGATATCCCCCCTAACTAATTTTg                                                                                                      >  6:54238/1‑148 (MQ=255)
                                                aaTAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTa                                                                                                    <  5:74789/149‑1 (MQ=255)
                                                aaTAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTa                                                                                                    <  3:302865/149‑1 (MQ=255)
                                                     aCATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGcc                                                                                               <  8:386008/149‑1 (MQ=255)
                                                       aTCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGAAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTa                                                                                             <  5:45746/149‑1 (MQ=255)
                                                          aGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACAt                                                                                          >  7:365140/1‑149 (MQ=255)
                                                            cTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTa                                                                                        <  2:102720/149‑1 (MQ=255)
                                                               tATCACCATGCTGATGCAAAGTGTGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGACAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATg                                                                                      <  2:270140/148‑1 (MQ=255)
                                                               tATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTAt                                                                                       <  8:365140/147‑1 (MQ=255)
                                                               tATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGc                                                                                     <  7:233780/149‑1 (MQ=255)
                                                               tATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGc                                                                                     >  5:357868/1‑149 (MQ=255)
                                                                aTCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCt                                                                                    >  6:40209/1‑149 (MQ=255)
                                                                  cACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTc                                                                                   >  7:144115/1‑148 (MQ=255)
                                                                  cACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTc                                                                                   >  1:331069/1‑148 (MQ=255)
                                                                     cATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGc                                                                                                                   >  2:195936/1‑113 (MQ=255)
                                                                     cATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGc                                                                                                                   <  1:195936/113‑1 (MQ=255)
                                                                     cATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCaat                                                                                >  7:264707/1‑148 (MQ=255)
                                                                        gCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCaataat                                                                             >  5:312881/1‑148 (MQ=255)
                                                                          tGATGCAAATTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGTTGTCAATAATTGAGAGTTCTCGCGATCCATTATTGACGCCTGATCTCCCGACTAACTTATTTTGTACTGCCTACATTATTCTCAAtaattaa                                                                          >  1:73372/1‑149 (MQ=255)
                                                                            aTGCAAAGTGAGCATTTTAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAAtaattaa                                                                          <  1:6865/147‑1 (MQ=255)
                                                                            aTGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAAtaattaa                                                                          <  1:146797/147‑1 (MQ=255)
                                                                            aTGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTGCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAAtaattaat                                                                         <  1:166503/148‑1 (MQ=255)
                                                                              gCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTACATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATggg                                                                      >  1:339882/1‑149 (MQ=255)
                                                                               cAAAGTTAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATCTCAGGTGTCAAGAAGGGAGATTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTCCCTTATTTTGTACTGCCTACCTTATGCTCAATAATTAATGGGt                                                                     <  2:73372/149‑1 (MQ=255)
                                                                               cAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATggg                                                                      <  3:144998/148‑1 (MQ=255)
                                                                               cAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGt                                                                     <  5:113921/149‑1 (MQ=255)
                                                                                aaaGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGtt                                                                    <  4:237129/149‑1 (MQ=255)
                                                                                 aaGTGCGCACTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCGCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTa                                                                   <  6:39407/149‑1 (MQ=255)
                                                                                 aaGTGATCATATCAGGCGTTATTCATACTTATTATGTCCGCAATATCAGGCGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTTATATCCCTCCTAACTTATTTTTTACTGCCTACATTATGCTCAATAATTAATGGGTTa                                                                   <  4:318676/149‑1 (MQ=255)
                                                                                 aaGTGAGCATTTCTGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTa                                                                   <  6:34312/149‑1 (MQ=255)
                                                                                       gCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATc                                                               >  4:209331/1‑147 (MQ=255)
                                                                                         aTTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGa                                                           <  3:25960/149‑1 (MQ=255)
                                                                                            tCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATg                                                         <  1:166636/148‑1 (MQ=255)
                                                                                              aGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATg                                                         >  5:221556/1‑146 (MQ=255)
                                                                                              aGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGga                                                       <  2:320840/148‑1 (MQ=255)
                                                                                              aGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGga                                                       <  2:331069/148‑1 (MQ=255)
                                                                                                   ttATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATgg                                                                       >  2:109130/1‑127 (MQ=255)
                                                                                                   ttATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATgg                                                                       <  1:109130/127‑1 (MQ=255)
                                                                                                         tttCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATc                                           >  3:251325/1‑149 (MQ=255)
                                                                                                            cTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCtt                                         <  7:102486/148‑1 (MQ=255)
                                                                                                              tattatGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCgg                                      >  6:110567/1‑149 (MQ=255)
                                                                                                               attatGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGc                                                                                                                               >  6:391592/1‑59 (MQ=255)
                                                                                                                  atGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGt                                   <  8:379677/148‑1 (MQ=255)
                                                                                                                  atGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGt                                   <  1:187507/148‑1 (MQ=255)
                                                                                                                   tGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTg                                  <  2:339882/148‑1 (MQ=255)
                                                                                                                   tGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATGTTATCTTCGGCGTgg                                 >  7:23152/1‑149 (MQ=255)
                                                                                                                     tCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGa                                >  2:199515/1‑148 (MQ=255)
                                                                                                                      ccGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCaa                                                                                 >  1:213498/1‑98 (MQ=255)
                                                                                                                      ccGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCaa                                                                                 <  2:213498/98‑1 (MQ=255)
                                                                                                                       cGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGACTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGatat                             >  4:31768/1‑149 (MQ=255)
                                                                                                                        gggatatCAGGCGTCAAGAATGGAGAGCGAGAACTCTCCATTCTTGACACCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATAtt                            >  4:376262/4‑149 (MQ=25)
                                                                                                                        gCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATAtt                            >  7:233701/1‑149 (MQ=255)
                                                                                                                             atCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTa                       >  4:100642/1‑149 (MQ=255)
                                                                                                                              tCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTa                       <  6:125325/148‑1 (MQ=255)
                                                                                                                              tCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTAt                      <  7:39868/149‑1 (MQ=255)
                                                                                                                              tCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTAt                      >  4:337343/1‑149 (MQ=255)
                                                                                                                              tCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGACATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTAt                      <  7:67001/149‑1 (MQ=255)
                                                                                                                                    atataGAATGGTTTGTTCTCGGTCTCCATTCTTGAAGACTTATTTGCCGGCTTACTTATTTTTTACTTGCTACATTAAGCTAAATAATTAATGGGTTAGATGAAGTTGGATAAAATTTTATCTTCGGCGTGGATATTTCTTATGctctc                <  7:216817/145‑1 (MQ=255)
                                                                                                                                       aaGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTAc             >  6:127918/1‑149 (MQ=255)
                                                                                                                                       aaGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTAc             >  4:260610/1‑149 (MQ=255)
                                                                                                                                        aGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTAc             >  5:66477/1‑148 (MQ=255)
                                                                                                                                         gAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCa           >  2:164701/1‑149 (MQ=255)
                                                                                                                                         gAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCa           <  3:94511/149‑1 (MQ=255)
                                                                                                                                         gAATGGAGAGATCTCGCTCTCCATACATGACCCAAGATATCCCGCATAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATTCGTTACATAAAGATGGATAAAATTTTAACTTCGGCGTGGATATTACTTATGCTATCTagat           >  8:216817/1‑146 (MQ=255)
                                                                                                                                          aaTGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCAt          <  6:393466/149‑1 (MQ=255)
                                                                                                                                          aaTGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCAt          <  8:233701/149‑1 (MQ=255)
                                                                                                                                              gagaGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTTTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGct       <  8:235448/148‑1 (MQ=255)
                                                                                                                                              gagaGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATgg                                                                       >  6:165474/1‑84 (MQ=255)
                                                                                                                                              gagaGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATgg                                                                       <  5:165474/84‑1 (MQ=255)
                                                                                                                                               agagTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGctct     <  2:113401/149‑1 (MQ=255)
                                                                                                                                                gagTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGGGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGctctc    <  4:45846/149‑1 (MQ=255)
                                                                                                                                                 agTGCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGctctct   <  2:316419/149‑1 (MQ=255)
                                                                                                                                                   ttCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGCTCTCTg  >  8:101549/1‑148 (MQ=255)
                                                                                                                                                   ttCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGCTCTCTg  >  7:283103/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCGCCTAACTTATTTTGTACTGCCTACATTATGCTCAATAATTAATGGGTTAGATCAAGATGGAGAAAATTTTATCTTCGGCGTGGATATTTCTTATGCTCTCTACCATGCTCTCTG  >  NC_000913/1216968‑1217262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: