Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,313,034 A→G 5.2% S210S (TCT→TCC)  yciC ← UPF0259 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,313,0340AG 5.2% 242.0 / 2.9 77S210S (TCT→TCC) yciCUPF0259 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base G (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

CATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGT  >  NC_000913/1312888‑1313181
                                                                                                                                                  |                                                                                                                                                   
cATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCaaaa                                                                                                                                                     <  8:185079/147‑1 (MQ=255)
  ttttACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAaagaag                                                                                                                                                 <  5:348131/149‑1 (MQ=255)
     tACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAagag                                                                                                                                               <  5:90349/148‑1 (MQ=255)
      aCGATTCCGTAATCAAATGCTTAAGCTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAgg                                                                                                                                              <  7:170895/148‑1 (MQ=255)
              gTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGCAGAGGCAAAAag                                                                                                                                       >  2:153091/1‑147 (MQ=255)
              gTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAagca                                                                                                                                     <  5:266340/149‑1 (MQ=255)
                  tCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAagag                                                                                                                                               >  2:78333/1‑135 (MQ=255)
                  tCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAagag                                                                                                                                               <  1:78333/135‑1 (MQ=255)
                     aaTGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAg                                                                                                                              >  3:344782/1‑149 (MQ=255)
                      aTGCTTAAGGTTATTGGCGAATCAACATATTCAGGCGTAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAgt                                                                                                                             <  2:119676/149‑1 (MQ=255)
                       tGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAgtg                                                                                                                            >  1:392582/1‑149 (MQ=255)
                          ttAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAagca                                                                                                                                     <  7:232982/137‑1 (MQ=255)
                          ttAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAagca                                                                                                                                     >  8:232982/1‑137 (MQ=255)
                            aaGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGttttt                                                                                                                       >  1:70938/1‑149 (MQ=255)
                             aGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGttttt                                                                                                                       <  3:262962/148‑1 (MQ=255)
                               gTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGcc                                                                                                                    <  8:382347/149‑1 (MQ=255)
                               gTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGc                                                                                                                     >  3:105202/1‑148 (MQ=255)
                                         aaTCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGc                                                                                                          >  7:45635/1‑149 (MQ=255)
                                         aaTCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCAAGCAACCAGc                                                                                                          >  8:277385/1‑149 (MQ=255)
                                           tCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCt                                                                                                         >  7:81895/1‑148 (MQ=255)
                                           tCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTGAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCt                                                                                                         >  7:76248/1‑148 (MQ=255)
                                             aaCATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCa                                                                                                       <  4:320120/148‑1 (MQ=255)
                                             aaCATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAg                                                                                                      >  7:247074/1‑149 (MQ=255)
                                               cATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTa                                                                                                    <  7:277385/149‑1 (MQ=255)
                                               cATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTa                                                                                                    <  3:34548/149‑1 (MQ=255)
                                                   taCAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACTGTGTTTTTGCCAGCAACCAGCTCAGTACTGc                                                                                                <  8:79272/149‑1 (MQ=255)
                                                   taCAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCa                                                                                                            <  6:145168/137‑1 (MQ=255)
                                                   taCAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCa                                                                                                            >  5:145168/1‑137 (MQ=255)
                                                   taCAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGc                                                                                                >  7:79272/1‑149 (MQ=255)
                                                   taCAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGGTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGc                                                                                                <  1:5889/149‑1 (MQ=255)
                                                    aCAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCg                                                                                               >  7:272594/1‑149 (MQ=255)
                                                            aaCAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGccac                                                                                       <  8:264436/149‑1 (MQ=255)
                                                            aaCAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGccac                                                                                       <  8:247074/149‑1 (MQ=255)
                                                                 atagatGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGAc                                                                                  >  5:219045/1‑149 (MQ=255)
                                                                     atGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCa                                                                               >  1:96541/1‑148 (MQ=255)
                                                                            atatGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGcc                                                                       <  8:272594/149‑1 (MQ=255)
                                                                            atatGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGcc                                                                       <  2:103297/149‑1 (MQ=255)
                                                                             tatGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGcc                                                                       >  3:209537/1‑148 (MQ=255)
                                                                                   tGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGt                                                                  >  7:288140/1‑147 (MQ=255)
                                                                                   tGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGt                                                                  >  3:365277/1‑147 (MQ=255)
                                                                                    gAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAGGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCaa                                                                    >  7:86166/1‑144 (MQ=255)
                                                                                    gAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAGGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCaa                                                                    >  7:80666/1‑144 (MQ=255)
                                                                                    gAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAGGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCaa                                                                    <  8:86166/144‑1 (MQ=255)
                                                                                    gAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAGGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCaa                                                                    <  8:80666/144‑1 (MQ=255)
                                                                                      aaTCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCgg                                                                                              >  3:288559/1‑116 (MQ=255)
                                                                                      aaTCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCgg                                                                                              <  4:288559/116‑1 (MQ=255)
                                                                                          aGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCa                                                          >  4:221316/1‑148 (MQ=255)
                                                                                          aGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCa                                                          >  4:375761/1‑148 (MQ=255)
                                                                                               gCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTg                                                     <  3:301117/148‑1 (MQ=255)
                                                                                                cTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGc                                                    <  8:288140/148‑1 (MQ=255)
                                                                                                   aaGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATa                                                        >  8:267737/1‑141 (MQ=255)
                                                                                                   aaGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATa                                                        <  7:267737/141‑1 (MQ=255)
                                                                                                   aaGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGGTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTa                                                  <  2:146585/147‑1 (MQ=255)
                                                                                                   aaGGTGGTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGCAGCGGCCAACAGCCGCAACAGTGTTTTTGCCAGCCCCCAGCTCAGTACTGCGGGTGACACCAGACGCCTATTCGCCCAAGTCAGCCGCATACTGCTGCg                                                >  2:116337/1‑149 (MQ=255)
                                                                                                     ggTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCa                                              <  8:52944/149‑1 (MQ=255)
                                                                                                     ggTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCa                                              >  1:239199/1‑149 (MQ=255)
                                                                                                      gtgtTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCTACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCAt                                             <  4:344782/149‑1 (MQ=255)
                                                                                                        gtTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTa                                                  <  4:84372/142‑1 (MQ=255)
                                                                                                        gtTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTAcagctcg                                           >  3:84372/1‑143 (MQ=255)
                                                                                                         ttCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATc                                            >  3:209210/1‑147 (MQ=255)
                                                                                                         ttCGCCAGTAAGGCAACAATTTCCGGGGTTAATGCGGCAAAAGAATAGTCAAAAATCAGCAACTGTGTTTTTGCCAGAAACCAGCTCTGTACTGCGGGTGCCATCAGACGCATATTCGCCCAACTCAGCCGCATACTGGTACGCATCGa                                          <  5:343971/149‑1 (MQ=255)
                                                                                                           cGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAg                                         <  4:105202/148‑1 (MQ=255)
                                                                                                            gCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAgg                                        <  4:365277/148‑1 (MQ=255)
                                                                                                              cAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCa                                      >  1:51820/1‑148 (MQ=255)
                                                                                                                 tACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCaaaac                                  <  6:179294/149‑2 (MQ=255)
                                                                                                                   cGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACg                                <  8:81895/149‑1 (MQ=255)
                                                                                                                   cGGAACCAATTTCCGGGGTAAATGCGGCAAAAGAAGAGGCAAATAGCAGCAACAGTGTTTTTGACAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGAAAAAACg                                <  8:76248/149‑1 (MQ=255)
                                                                                                                      cACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGcc                              >  4:58057/1‑148 (MQ=255)
                                                                                                                      cACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGACCGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGcc                              >  7:319372/1‑148 (MQ=255)
                                                                                                                      cACCAATTTCAGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCATCTCAGTACTGCGGGTGCCACCAGACGCATATTAGCCCAAGTCATCCGCATACTGCTACGCATCGAGTCAAACACGcc                              >  3:91521/1‑148 (MQ=255)
                                                                                                                         cAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTCCGCATCGAGGCAAAAACGCCCAtt                          >  1:356878/1‑149 (MQ=255)
                                                                                                                          aaTTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAttt                         >  1:204788/1‑149 (MQ=255)
                                                                                                                           aTTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGGATACAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAtttt                        <  5:318071/149‑1 (MQ=255)
                                                                                                                           aTTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCAtttt                        <  7:23002/149‑1 (MQ=255)
                                                                                                                              tCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGt                      <  2:204788/148‑1 (MQ=255)
                                                                                                                                   ggTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTa                >  2:20902/1‑149 (MQ=255)
                                                                                                                                   ggTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTa                <  2:70938/149‑1 (MQ=255)
                                                                                                                                            ggCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCa       >  4:357198/1‑149 (MQ=255)
                                                                                                                                              cAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCAcc     >  4:366630/1‑149 (MQ=255)
                                                                                                                                               aaaaGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCAGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCg    >  5:222018/1‑149 (MQ=255)
                                                                                                                                               aaaaGAAGAGGCAAAAAGCAGCAACAGTGGTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGAAGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCg    <  8:362971/149‑1 (MQ=255)
                                                                                                                                                aaaGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCgg   >  5:148837/1‑149 (MQ=255)
                                                                                                                                                 aagaagAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGt  <  3:357198/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGGAACAGATAGATGAGCAATATGGCTGAAATCAGGTTGCTCAAGGTGTTCGCCAGTACGGCACCAATTTCCGGGGTTAATGCGGCAAAAGAAGAGGCAAAAAGCAGCAACAGTGTTTTTGCCAGCAACCAGCTCAGTACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGT  >  NC_000913/1312888‑1313181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑