Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,988,048 A→T 6.5% intergenic (‑89/‑174) azuC ← / → yecR acid‑inducible small membrane‑associated protein/lipoprotein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,988,0480AT 6.5% 235.3 / 5.9 77intergenic (‑89/‑174)azuC/yecRacid‑inducible small membrane‑associated protein/lipoprotein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (34/37);  new base T (2/3);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTG  >  NC_000913/1987902‑1988195
                                                                                                                                                  |                                                                                                                                                   
cTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACa                                                                                                                                                   <  1:331215/149‑1 (MQ=255)
 tttGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACa                                                                                                                                                   >  6:156507/1‑148 (MQ=255)
      aCGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATca                                                                                                                                              >  3:136433/1‑148 (MQ=255)
        gTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACAt                                                                                                                                           <  7:15402/149‑1 (MQ=255)
           ttGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATaaa                                                                                                                                        >  4:150022/1‑149 (MQ=255)
             gCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATa                                                                                                                                      >  5:306970/1‑149 (MQ=255)
              cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCTCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaaga                                                                                                                                      <  2:3399/148‑3 (MQ=255)
              cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaaga                                                                                                                                      <  5:187172/148‑3 (MQ=255)
                   gTTAATCAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACGCCGACCATTTGTTATACAGGTGTAAATGTCTTTGCGCTTATAATCTGCCAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCtt                                                                                                                                <  2:108131/149‑1 (MQ=255)
                         gAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATg                                                                                                                           <  6:200767/148‑1 (MQ=255)
                         gAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATg                                                                                                                           <  3:352109/148‑1 (MQ=255)
                          aaCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt                                                                                                                         >  2:59953/1‑149 (MQ=255)
                          aaCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGt                                                                                                                          <  8:251719/148‑1 (MQ=255)
                           aCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt                                                                                                                         <  6:306970/148‑1 (MQ=255)
                           aCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTta                                                                                                                        <  1:87746/149‑1 (MQ=255)
                           aCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTta                                                                                                                        >  3:179030/1‑149 (MQ=255)
                            cATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTta                                                                                                                        >  1:7745/1‑148 (MQ=255)
                                     cAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTACAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGa                                                                                                               >  1:76636/1‑148 (MQ=255)
                                      aGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATa                                                                                                             <  7:256773/149‑1 (MQ=255)
                                         aTTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATc                                                                                                          <  4:207423/149‑1 (MQ=255)
                                         aTTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATc                                                                                                          <  8:373105/149‑1 (MQ=255)
                                               cgcAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTTCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCaaaaaa                                                                                                    <  5:331261/149‑1 (MQ=255)
                                               cgcAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCaaaaaa                                                                                                    <  2:246531/149‑1 (MQ=255)
                                                 cAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCaaaaaa                                                                                                    >  5:330117/1‑147 (MQ=255)
                                                  aGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAg                                                                                                 <  4:136433/149‑1 (MQ=255)
                                                  aGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAg                                                                                                 <  8:293322/149‑1 (MQ=255)
                                                        aTCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACCCCCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGcccccc                                                                                           >  5:96174/1‑149 (MQ=255)
                                                         tCGCGGCACTCCGACCATTTGTTATACAGGTTTTATTGTCTTTGCGCTTATAATATTAGAACCATGTCAATATTCATTCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAATAATGATAATCAAAAAACAGCCCCCCt                                                                                          <  7:237287/149‑1 (MQ=255)
                                                           gcgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt                                                                                                                         >  3:130709/1‑116 (MQ=255)
                                                           gcgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt                                                                                                                         <  4:130709/116‑1 (MQ=255)
                                                               cACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCt                                                                                    <  7:14461/149‑1 (MQ=255)
                                                                aCTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCt                                                                                    >  1:32262/1‑148 (MQ=255)
                                                                 cTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGa                                                                                  <  3:254305/149‑1 (MQ=255)
                                                                  tCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGa                                                                                  >  6:167329/1‑148 (MQ=255)
                                                                  tCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGa                                                                                  >  1:388814/1‑148 (MQ=255)
                                                                    cGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGacac                                                                               >  6:64607/1‑149 (MQ=255)
                                                                    cGACCAGTTGTTATACAGGTGTTATTGTCTTTGAGCTTCTAATATGACAACCATAACAACAATCAATCTTTATGTGATCCACATCACATAAATACCCCTTTAATGTTATAAAAATGCTCATCAAACAACAGCCCCCCTATTTCTGaca                                                                                <  4:76356/148‑1 (MQ=255)
                                                                    agcccATTTGTTCTACATCTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAACTCACTCTTTATGTGATACACATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGaca                                                                                <  6:276261/145‑1 (MQ=255)
                                                                        cATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTAc                                                                           <  3:326738/149‑1 (MQ=255)
                                                                          tttGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGc                                                                                                <  8:11553/126‑1 (MQ=255)
                                                                          tttGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGc                                                                                                >  7:11553/1‑126 (MQ=255)
                                                                              ttATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCACCCCTATTTCTGACACGTACAGATgg                                                                     >  6:255288/1‑149 (MQ=255)
                                                                                  aCAGGTGTTATTGTCTTTGCGCTTATAATTTGGCATCCATCAAAAAAATCAATCTTTATGTGATACAAATCACATAAATTCACCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCGTATTTCGGACTCCGACCGATggg                                                                    >  2:308229/1‑145 (MQ=255)
                                                                                  aCAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGc                                                                    >  3:254870/1‑146 (MQ=255)
                                                                                         ttATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgc                                                          <  4:179030/149‑1 (MQ=255)
                                                                                         ttATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgc                                                          >  5:92232/1‑149 (MQ=255)
                                                                                            ttGTCTTTGCGCTTATAATATGACAACCATCACAATATTCAATCTTTATGTGATACATCTGACATAAATACCCCTTTAATGTTATAAAAATGATAATGAAAAAAAAGCCCCCCTATTTCTGAACCCTACAGATCGCAAGAAATAGCGcc                                                       >  1:210466/1‑149 (MQ=255)
                                                                                               tCTTTGCGCTTATAATATGACAACCATCAAAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATTATAATCAAAAAACAGCCCCCCGATTTCTGACACCTACAGATGGCAAGAAATAGCGCCAGc                                                    >  8:276361/1‑149 (MQ=255)
                                                                                                           caataTGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCttt                                         <  2:76636/147‑1 (MQ=255)
                                                                                                             atatGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTcc                                      >  2:111692/1‑149 (MQ=255)
                                                                                                              tatGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACCGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCg                                     >  4:19400/1‑149 (MQ=255)
                                                                                                              tatGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCg                                     >  5:9173/1‑149 (MQ=255)
                                                                                                              tatGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCg                                     <  5:156507/149‑1 (MQ=255)
                                                                                                                   cAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCAt                                >  8:356580/1‑149 (MQ=255)
                                                                                                                      ccATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCttt                                         >  1:235619/1‑137 (MQ=255)
                                                                                                                      ccATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCttt                                         <  2:235619/137‑1 (MQ=255)
                                                                                                                      ccATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGt                             >  2:58548/1‑149 (MQ=255)
                                                                                                                       cATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAAAGTTATAAAAATGATAATCAAAAAACAGCCCCAATATTTGTGACACCTACAGATGGCAAGATATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTc                            >  8:357615/1‑149 (MQ=255)
                                                                                                                        aTCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTTTCg                           >  5:220162/1‑149 (MQ=255)
                                                                                                                           acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgcag                        <  2:7745/149‑1 (MQ=255)
                                                                                                                           acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgca                         <  6:92232/148‑1 (MQ=255)
                                                                                                                           acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgca                         <  6:94265/148‑1 (MQ=255)
                                                                                                                           acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgca                         <  6:330117/148‑1 (MQ=255)
                                                                                                                           acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATATCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgca                         <  2:80164/148‑1 (MQ=255)
                                                                                                                             aaaaaTCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgcagca                      <  6:96174/149‑1 (MQ=255)
                                                                                                                              aaaaTCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCAc                     <  2:205648/149‑1 (MQ=255)
                                                                                                                                   caatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCACCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTTCCAGGCGTATTTTCCGGCCATTGTCGCAGAACTGTaa                >  6:297751/1‑149 (MQ=255)
                                                                                                                                    ggtatttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCAt                                <  7:232711/128‑1 (MQ=255)
                                                                                                                                    ggtatttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCAt                                >  8:232711/5‑132 (MQ=255)
                                                                                                                                      tcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCaa                                                                  <  8:122917/96‑1 (MQ=255)
                                                                                                                                      tcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCaa                                                                  >  7:122917/1‑96 (MQ=255)
                                                                                                                                      tatttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAcgc             >  3:29204/3‑149 (MQ=255)
                                                                                                                                        tttATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAcgcg            >  1:188081/1‑148 (MQ=255)
                                                                                                                                              tgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATa     <  1:59953/149‑1 (MQ=255)
                                                                                                                                              tgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATa     <  4:50075/149‑1 (MQ=255)
                                                                                                                                              tgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATa     <  5:64607/149‑1 (MQ=255)
                                                                                                                                                 tACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTg  >  8:310295/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTG  >  NC_000913/1987902‑1988195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: