Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,378,156 G→A 5.7% G368G (GGC→GGT menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,378,1560GA 5.7% 222.0 / 2.4 70G368G (GGC→GGTmenD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
Reads supporting (aligned to +/- strand):  ref base G (39/27);  new base A (2/2);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

CCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGG  >  NC_000913/2378010‑2378300
                                                                                                                                                  |                                                                                                                                                
ccGCTGGCCCCACGGTTGCTGTACACCGTGTAACCTGCCGGAAGTTGCGAAAGCGCATCTATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGc                                                                                                                                                 <  3:26603/148‑1 (MQ=255)
 cGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAAACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGcc                                                                                                                                                >  8:386433/1‑148 (MQ=255)
   cTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAAACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCaaa                                                                                                                                             <  7:386433/149‑1 (MQ=255)
         ccACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATAAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCAAGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCAt                                                                                                                                        >  2:168397/1‑148 (MQ=255)
            cGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcg                                                                                                                                    <  6:373454/149‑1 (MQ=255)
                 gCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcggcgg                                                                                                                                <  5:202222/148‑1 (MQ=255)
                  cTGTACACCTGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCggg                                                                                                                               <  5:176721/148‑1 (MQ=255)
                  cTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCggg                                                                                                                               <  3:312348/148‑1 (MQ=255)
                   tGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGc                                                                                                                              >  5:342072/1‑148 (MQ=255)
                   tGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGAAGCGCAACTGGCGCATCGc                                                                                                                                                 >  5:341104/1‑129 (MQ=255)
                   tGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGAAGCGCAACTGGCGCATCGc                                                                                                                                                 <  6:341104/129‑1 (MQ=255)
                        acCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAAc                                                                                                                        <  3:148014/149‑1 (MQ=255)
                        acCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAAc                                                                                                                        >  1:65034/1‑149 (MQ=255)
                        acCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAAc                                                                                                                        <  5:256095/149‑1 (MQ=255)
                          cGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCg                                                                                                                      >  4:341256/1‑149 (MQ=255)
                              tAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTg                                                                                                                  >  4:377596/1‑149 (MQ=255)
                                      cGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCt                                                                                                          <  2:29473/149‑1 (MQ=255)
                                               cGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCa                                                                                                 <  2:277544/149‑1 (MQ=255)
                                                  aaGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGgc                                                                                              >  4:302663/1‑149 (MQ=255)
                                                      gcATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCgg                                                                                          >  5:70644/1‑149 (MQ=255)
                                                         tcaatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGAt                                                                                       >  8:382579/1‑149 (MQ=255)
                                                          caatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGAtt                                                                                      >  6:162807/1‑149 (MQ=255)
                                                          caatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCACCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTc                                                                                                      <  7:258412/133‑1 (MQ=255)
                                                          caatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCACCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTc                                                                                                      >  2:247573/1‑133 (MQ=255)
                                                          caatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCACCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTc                                                                                                      >  8:258412/1‑133 (MQ=255)
                                                          caatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCACCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTc                                                                                                      <  1:247573/133‑1 (MQ=255)
                                                           aatcaGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGAttt                                                                                     <  6:342072/149‑1 (MQ=255)
                                                               aGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCaa                                                                                  <  2:65034/148‑1 (MQ=255)
                                                               aGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAAc                                                                                 <  6:313385/149‑1 (MQ=255)
                                                               aGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAAc                                                                                 >  5:49551/1‑149 (MQ=255)
                                                                   gTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCAc                                                                             >  5:371647/1‑149 (MQ=255)
                                                                        accaGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAggg                                                                        >  7:310279/1‑149 (MQ=255)
                                                                                 ttACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGttt                                                               <  5:162807/149‑1 (MQ=255)
                                                                                    ccAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                            >  1:194393/1‑149 (MQ=255)
                                                                                    ccAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                            >  8:377490/1‑149 (MQ=255)
                                                                                    ccAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                            >  3:79982/1‑149 (MQ=255)
                                                                                     caacaaACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAAc                                                                                 <  5:366033/127‑1 (MQ=255)
                                                                                     caacaaACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAAc                                                                                 >  6:366033/1‑127 (MQ=255)
                                                                                     caacaaACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCt                                                           <  6:316036/149‑1 (MQ=255)
                                                                                     caacaaACAATTGCCCCAGTTCAGGCAGATAGTCGCAGATCCGATGCGCCAGTAGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCAGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCAGGCGtttttat                                                           >  2:324479/1‑147 (MQ=255)
                                                                                       acaatcaaTTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCACCGCACCAGGGCTGGCGTTTTTCTGc                                                         >  6:138943/1‑149 (MQ=255)
                                                                                       acaaacaaTTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGc                                                         >  5:61676/1‑149 (MQ=255)
                                                                                         aaacaaTTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCg                                                       >  5:55534/1‑149 (MQ=255)
                                                                                         aaacaaTTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCg                                                       >  4:164812/1‑149 (MQ=255)
                                                                                         aaacaaTTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCAGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCg                                                       >  5:198563/1‑149 (MQ=255)
                                                                                           acaaTTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGa                                                     >  8:49099/1‑149 (MQ=255)
                                                                                               ttGCCCCTGTTCAGGCAGATAGTCGCAGATGCTATGCGCCAGTTGCGCTTCGCCAAACTCAGCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCa                                                 <  1:324479/149‑1 (MQ=255)
                                                                                                  ccccTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCt                                              >  6:241347/1‑149 (MQ=255)
                                                                                                   cccTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTc                                             >  1:308506/1‑149 (MQ=255)
                                                                                                     cTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTc                                             >  3:86326/1‑147 (MQ=255)
                                                                                                      tGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAACCGCATCACGGCGGGCAATACCCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccag                                          >  6:283575/1‑149 (MQ=255)
                                                                                                      tGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccag                                          >  1:336833/1‑149 (MQ=255)
                                                                                                        ttCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcc                                            >  8:368187/1‑145 (MQ=255)
                                                                                                        ttCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcc                                            <  7:368187/145‑1 (MQ=255)
                                                                                                          cAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                                      >  8:83868/1‑149 (MQ=255)
                                                                                                          cAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                                      >  7:364893/1‑149 (MQ=255)
                                                                                                          cAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                                      >  7:311683/1‑149 (MQ=255)
                                                                                                          cAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                                      >  3:368342/1‑149 (MQ=255)
                                                                                                             gCAGATAGTCGCAGATGCGATGCTCCAGTTGCGCTTCGCCAAACGCTTCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGTTTTCAACGCTCCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCg                                   <  1:168397/149‑1 (MQ=255)
                                                                                                             gCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCg                                   <  8:364893/149‑1 (MQ=255)
                                                                                                              cAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGTGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCgg                                  <  7:83868/149‑1 (MQ=255)
                                                                                                               aGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCgg                                  >  8:165894/1‑148 (MQ=255)
                                                                                                                 aTAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCaa                               >  8:301346/1‑149 (MQ=255)
                                                                                                                    gTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAata                             <  3:341256/148‑1 (MQ=255)
                                                                                                                          gatgcgatgcGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCaa                      <  3:302663/149‑1 (MQ=255)
                                                                                                                            tgcgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAtt                    <  7:301346/149‑1 (MQ=255)
                                                                                                                              cgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTaa                  <  6:70644/149‑1 (MQ=255)
                                                                                                                              cgatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTaa                  <  4:79982/149‑1 (MQ=255)
                                                                                                                               gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGACGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAg                 >  7:132082/1‑149 (MQ=255)
                                                                                                                                              cTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCCGTCGGCAATATTGGCAATTAAGCGACGTCCGCGCTgg  >  8:10847/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGG  >  NC_000913/2378010‑2378300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: