Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,715,258 A→G 6.6% A21A (GCT→GCC) ‡ yfiE ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,715,2580AG 6.6% 320.7 / 9.8 107A21A (GCT→GCC) ‡yfiEputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (48/51);  new base G (5/2);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 111 total aligned reads displayed.

GTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACC  >  NC_000913/2715110‑2715403
                                                                                                                                                    |                                                                                                                                                 
gTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGaa                                                                                                                                                   <  3:36865/149‑1 (MQ=255)
gTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGaa                                                                                                                                                   <  1:112564/149‑1 (MQ=255)
gTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGGCGATTGTGTACAGCACAATTTTTGCGaa                                                                                                                                                   <  1:117908/149‑1 (MQ=255)
 ttCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAg                                                                                                                                                  >  7:31295/1‑149 (MQ=255)
       aaTGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGc                                                                                                                                             <  7:31907/148‑1 (MQ=255)
       aaTGGGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGc                                                                                                                                             <  2:245185/148‑1 (MQ=255)
        aTGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACATCACAATTTTTGCGAAGCTCGCa                                                                                                                                            >  3:320509/1‑148 (MQ=255)
           tgCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCTCAATTTTTGCGAAGCTCGCaaaaa                                                                                                                                        <  8:104070/149‑1 (MQ=255)
             cGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCATTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAGCTTCGCaaaaa                                                                                                                                        <  7:150973/147‑1 (MQ=255)
             cGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAGCGTCGCaaaaa                                                                                                                                        >  8:150973/1‑147 (MQ=255)
                cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAAc                                                                                                                                   <  6:141822/149‑1 (MQ=255)
                   caACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCct                                                                                                                                >  8:251029/1‑149 (MQ=255)
                    aaCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCctc                                                                                                                               >  3:94887/1‑149 (MQ=255)
                    aaCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCctc                                                                                                                               >  7:14883/1‑149 (MQ=255)
                    aaCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAACCTCGCAAAAAGGTACCctc                                                                                                                               >  6:107446/1‑149 (MQ=255)
                             ccTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCcaccac                                                                                                                      >  6:209157/1‑149 (MQ=255)
                             ccTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCcaccac                                                                                                                      >  4:340017/1‑149 (MQ=255)
                                  gcgGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGttt                                                                                                                 <  8:31295/149‑1 (MQ=255)
                                   cgtgTAAGGCACATCCGTAGTCCAATTTTCTCAATTAACTGGACTGAAAACTCCTTCTCATGCTGCTGAATATGAAAAGTCTCCGTCTATTGTGTTCAGCACTATTTTTGCGAAGGTCGCAAAAAGGAACCCTCTTGCACCACGGtttt                                                                                                                <  8:132026/146‑1 (MQ=255)
                                   cgGGTAAGGCACATCCGTCGGGCAATTTTCTCAAATATCTGGACTGAAAACTCCTGCTCTAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGTtttt                                                                                                                <  2:33105/149‑1 (MQ=255)
                                     ggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGtttt                                                                                                                >  2:223749/1‑147 (MQ=255)
                                     ggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTaa                                                                                                              <  2:96575/149‑1 (MQ=255)
                                     ggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTaa                                                                                                              <  1:312268/149‑1 (MQ=255)
                                     ggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTaa                                                                                                              <  8:270042/149‑1 (MQ=255)
                                       tAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACACCTCCACAAGCTGCTGAATATGAAAAGTCACCGTCGATTCTGTACAGAACAATTTTTGCGACGCTCGCAAAAAGGAACCCTCTTCCACCAAGGTTTTAAGc                                                                                                            >  4:126879/1‑149 (MQ=255)
                                          ggCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGttt                                                                                                                 <  5:342543/141‑1 (MQ=255)
                                          ggCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGttt                                                                                                                 >  6:342543/1‑141 (MQ=255)
                                           gCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTCGCGAGCTTCGCaaaaa                                                                                                                                        <  1:200467/117‑1 (MQ=255)
                                           gCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTCGCGAGCTTCGCaaaaa                                                                                                                                        >  2:200467/1‑117 (MQ=255)
                                            cacaTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGtaat                                                                                                       <  7:172130/149‑1 (MQ=255)
                                            cacaTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGtaat                                                                                                       <  7:371358/149‑1 (MQ=255)
                                             acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGaa                                                                                                                                    >  7:271079/1‑119 (MQ=255)
                                             acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGaa                                                                                                                                    <  8:271079/119‑1 (MQ=255)
                                               aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGtaataa                                                                                                     >  5:105801/1‑148 (MQ=255)
                                               aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGtaataa                                                                                                     >  7:290997/1‑148 (MQ=255)
                                               aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATaaa                                                                                                    <  7:251029/149‑1 (MQ=255)
                                                tCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATaaaa                                                                                                   <  3:122519/149‑1 (MQ=255)
                                                       gCCAATTTTCTCAAATAACTGTACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCCCCGTCGATTGTGTACAGCACAATTTTTGAGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGAGTAATAAAACGgcg                                                                                              >  4:348096/1‑147 (MQ=255)
                                                       gCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGgcg                                                                                              >  2:345869/1‑147 (MQ=255)
                                                        ccAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGgcg                                                                                              >  8:62212/1‑146 (MQ=255)
                                                          aaTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGAt                                                                                         <  1:21633/149‑1 (MQ=255)
                                                                  cAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGCTCCATAAcc                                                                                 >  8:329931/1‑149 (MQ=255)
                                                                   aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAccc                                                                                >  2:79856/1‑149 (MQ=255)
                                                                   aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAcac                                                                                >  6:254633/1‑147 (MQ=255)
                                                                   aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAc                                                                                  <  4:16772/147‑1 (MQ=255)
                                                                    aaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTa                                                                                                               >  7:160989/1‑117 (MQ=255)
                                                                    aaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTa                                                                                                               <  8:160989/117‑1 (MQ=255)
                                                                    aaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGGCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGACCCCTCTTCCCCCACGGTTTTAAGCGTAAGAAAACGGCGCAGCTCCATAAccc                                                                                >  6:316220/1‑148 (MQ=255)
                                                                               tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCg                                                                     >  1:370/1‑148 (MQ=255)
                                                                               tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCg                                                                     >  6:63838/1‑148 (MQ=255)
                                                                                   aaCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGAt                                                                                         >  1:270852/1‑124 (MQ=255)
                                                                                   aaCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGAt                                                                                         <  2:270852/124‑1 (MQ=255)
                                                                                           cTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAAt                                                         <  2:290189/148‑1 (MQ=255)
                                                                                           cTCAAGCTGATGATTATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTTCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAAt                                                         <  5:210241/148‑1 (MQ=255)
                                                                                              aaGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTGTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCTAACCTATCGAAAATATCGAATCTa                                                      <  4:320509/148‑1 (MQ=255)
                                                                                              aaGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCCCCCCGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATc                                                        >  1:235670/1‑146 (MQ=255)
                                                                                              aaGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATc                                                        >  7:313722/1‑146 (MQ=255)
                                                                                              aaGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATAGAATc                                                        >  6:258689/1‑146 (MQ=255)
                                                                                                gctggtGAATCTGAAAAGTCACCGGCGTTTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGAGTAATAAAACGGCGCAGATCGATAACCCCACACCTATCGAAAATATCGAATCTAGaa                                                   <  4:9293/149‑1 (MQ=255)
                                                                                                gctgctGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGaa                                                   <  3:340017/149‑1 (MQ=255)
                                                                                                gctgctGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGaa                                                   <  8:208762/149‑1 (MQ=255)
                                                                                                  tgctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATATCCCCAAACCTATCGAAAATATCGAATCTAGAata                                                 >  5:34330/1‑149 (MQ=255)
                                                                                                  tgctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAccc                                                                                <  7:212718/118‑1 (MQ=255)
                                                                                                  tgctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAccc                                                                                >  8:212718/1‑118 (MQ=255)
                                                                                                  tgctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAata                                                 >  1:23390/1‑149 (MQ=255)
                                                                                                     tgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAAtata                                               >  3:129607/1‑148 (MQ=255)
                                                                                                     tgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAAACATCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAAtata                                               >  2:36112/1‑148 (MQ=255)
                                                                                                      gAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaa                                              >  6:224305/1‑148 (MQ=255)
                                                                                                         tatGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaaaaa                                           >  5:13547/1‑148 (MQ=255)
                                                                                                             aaaaGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCGGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcatt                                       <  6:170804/148‑1 (MQ=255)
                                                                                                             aaaaGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcatt                                       >  1:328139/1‑148 (MQ=255)
                                                                                                                aGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcattcatt                                   >  5:289539/1‑149 (MQ=255)
                                                                                                                 gTCACCCTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATACCCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAttt                                  >  1:49106/1‑149 (MQ=255)
                                                                                                                  tCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAtttt                                 >  1:345882/1‑149 (MQ=255)
                                                                                                                   cACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAttttt                                <  4:94887/149‑1 (MQ=255)
                                                                                                                   cACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAttttt                                >  2:331321/1‑149 (MQ=255)
                                                                                                                    acagTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCAAGGTTTTACGCGTAATAAAACGTCGCAGATCCATAACCCCAAAGCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAttttt                                <  5:107446/145‑1 (MQ=255)
                                                                                                                      cGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaaaa                                            >  2:373325/1‑134 (MQ=255)
                                                                                                                      cGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaaaa                                            <  1:373325/134‑1 (MQ=255)
                                                                                                                      cGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTa                             <  5:63838/149‑1 (MQ=255)
                                                                                                                       gTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTaa                            <  6:289539/149‑1 (MQ=255)
                                                                                                                       gTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATAGAATCTAGAATATAAAAACATTCATTTTTTTaa                            <  6:105801/149‑1 (MQ=255)
                                                                                                                        tCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTaaa                           >  7:261572/1‑149 (MQ=255)
                                                                                                                              gtgtACAGCACAATTTTTGCTAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTc                      <  1:345869/148‑1 (MQ=255)
                                                                                                                              gtgtACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTc                      <  2:328139/148‑1 (MQ=255)
                                                                                                                              gtgtACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTc                      <  5:352368/148‑1 (MQ=255)
                                                                                                                                 tACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCgtg                  >  1:181137/1‑149 (MQ=255)
                                                                                                                                   cAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCTCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTc                <  3:348096/149‑1 (MQ=255)
                                                                                                                                   cAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTc                <  8:14883/149‑1 (MQ=255)
                                                                                                                                    aGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCg               >  2:41130/1‑149 (MQ=255)
                                                                                                                                     gCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATATAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCgg              <  3:42393/149‑1 (MQ=255)
                                                                                                                                      cacaATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCggg             >  5:208433/1‑149 (MQ=255)
                                                                                                                                      cacaATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCggg             >  3:125576/1‑149 (MQ=255)
                                                                                                                                        tcctttttGCGAGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGt            >  4:10331/4‑148 (MQ=255)
                                                                                                                                        caATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTa           <  2:370/149‑1 (MQ=255)
                                                                                                                                          ctttttGCGAGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACt         >  1:324829/2‑149 (MQ=255)
                                                                                                                                          aTTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACt         <  1:36112/149‑1 (MQ=255)
                                                                                                                                           tttttGCGAGCTTCGCAAAAAGGACCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTATTTTAAATGTTCCGTGTCGGGTACTg        >  1:196152/1‑149 (MQ=255)
                                                                                                                                              ttGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTc      <  8:261572/148‑1 (MQ=255)
                                                                                                                                                 cGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTAcc  <  1:223749/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACC  >  NC_000913/2715110‑2715403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: