Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 659,204 C→T 7.1% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,2040CT 7.1% 263.3 / 6.7 85intergenic (+54/+47)tatE/lipATatABCE protein translocation system subunit/lipoate synthase
Reads supporting (aligned to +/- strand):  ref base C (37/41);  new base T (4/2);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

AAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCG  >  NC_000913/659056‑659350
                                                                                                                                                    |                                                                                                                                                  
aaGGGTTCAAGAAGGCGATGAATCATGACGATGCAGCGGCGAAATAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGACCAGGACGCTCCCGCAATATCTTCTTCGATACAAAAAACCCCCTTc                                                                                                                                                    <  4:232712/149‑1 (MQ=255)
             ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGttt                                                                                                                                       >  4:140359/1‑149 (MQ=255)
             ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGttt                                                                                                                                       <  7:316124/149‑1 (MQ=255)
               cGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGCGTGACGTGGCGAGCAGGACGGTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttt                                                                                                                                     <  8:218333/149‑1 (MQ=255)
               cGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttt                                                                                                                                     <  4:375680/149‑1 (MQ=255)
                gATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGtttttt                                                                                                                                    <  6:378682/149‑1 (MQ=255)
                    aatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTAt                                                                                                                                  >  5:43254/1‑147 (MQ=255)
                    aatgaTGACGATGCGGCGGCGAAAAAAGGCGCAGACGTTGATCTTGAGGCGGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAATAGCGGGTTTTTTAtat                                                                                                                                <  2:318321/149‑3 (MQ=255)
                     atgatgACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATca                                                                                                                                <  4:183444/148‑1 (MQ=255)
                     atgatgACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATca                                                                                                                                <  6:52806/148‑1 (MQ=255)
                      tgatgaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATcaga                                                                                                                              <  6:174744/149‑1 (MQ=255)
                               tgctgcGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaa                                                                                                                     <  7:61181/149‑1 (MQ=255)
                                 ctgcGGCGAAAAAAGGCGGAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATACATAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaagt                                                                                                                   <  3:16690/149‑1 (MQ=255)
                                 ctgcGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCACGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaag                                                                                                                    <  5:88321/148‑1 (MQ=255)
                                    cggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAat                                                                                                                >  8:325857/1‑149 (MQ=255)
                                      gcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAatta                                                                                                              >  2:337553/1‑149 (MQ=255)
                                        gAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAattat                                                                                                             >  4:125512/1‑148 (MQ=255)
                                        gAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACACGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAattat                                                                                                             >  7:241084/1‑148 (MQ=255)
                                         aaaaaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAttatta                                                                                                           <  2:400528/149‑1 (MQ=255)
                                           aaaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAttatta                                                                                                           >  7:24893/1‑147 (MQ=255)
                                           aaaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACa                                                                                                         <  1:117338/149‑1 (MQ=255)
                                           aaaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACa                                                                                                         <  4:228551/149‑1 (MQ=255)
                                            aatggCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAg                                                                                                        <  1:153956/146‑1 (MQ=255)
                                             aaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAgg                                                                                                       <  1:218205/149‑1 (MQ=255)
                                             aaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAgg                                                                                                       <  3:125512/149‑1 (MQ=255)
                                                  gcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                  >  2:221470/1‑149 (MQ=255)
                                                  gcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                  >  1:14414/1‑149 (MQ=255)
                                                  gcAGACGGTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                  >  2:227740/1‑149 (MQ=255)
                                                    aGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACtt                                                                                                <  8:271981/149‑1 (MQ=255)
                                                        gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAAc                                                                                             >  8:91989/1‑148 (MQ=255)
                                                        gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAAc                                                                                             <  7:91989/148‑1 (MQ=255)
                                                        gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACt                                                                                            >  5:356976/1‑149 (MQ=255)
                                                         ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaa                                                                                                                     <  2:312552/123‑1 (MQ=255)
                                                         ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgtaa                                                                                                                     >  1:312552/1‑123 (MQ=255)
                                                         ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                           <  5:130643/149‑1 (MQ=255)
                                                            aTCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                        >  1:214885/1‑149 (MQ=255)
                                                             tCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                                                                 >  3:55671/1‑139 (MQ=255)
                                                             tCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                                                                 <  4:55671/139‑1 (MQ=255)
                                                                 cAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATc                                                                                      <  6:356976/146‑1 (MQ=255)
                                                                 cAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATc                                                                                      <  6:261549/146‑1 (MQ=255)
                                                                    gCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGCCGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCGTTc                                                                                >  5:72413/1‑149 (MQ=255)
                                                                        aaaaGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCt                                                                            >  8:298990/1‑149 (MQ=255)
                                                                         aaaGCTCTGTCATTTAGAGTGTCGTGGCGAGCAGGTCGGTCAGTCATTATCTTGTTCGATACAAAAAACCCGCTTCAATATGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAATTAACTTCCATGCCTTTCGCCTg                                                                           <  8:337035/149‑1 (MQ=255)
                                                                             ctctctCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCaaa                                                                       <  7:117011/149‑1 (MQ=255)
                                                                              tctctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAAt                                                                      >  8:97472/1‑149 (MQ=255)
                                                                              tctctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAAt                                                                      >  1:128171/1‑149 (MQ=255)
                                                                               ctctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATc                                                                     >  7:321250/1‑149 (MQ=255)
                                                                                  tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAATTCGGc                                                                  >  6:93808/1‑149 (MQ=255)
                                                                                  tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                                                                    <  2:14715/147‑1 (MQ=255)
                                                                                    aTAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                 <  3:140359/148‑1 (MQ=255)
                                                                                       aaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTgg                                                              <  5:217386/148‑1 (MQ=255)
                                                                                        agagTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTa                                                            <  2:214885/149‑1 (MQ=255)
                                                                                         gagTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaa                                                           <  7:97472/149‑1 (MQ=255)
                                                                                           gTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTATGTAATTATTACAGGATTACTTAACTTCCATCCCTTTAGCCTGCAAATCGGCGTGGTaag                                                          >  5:80807/1‑148 (MQ=255)
                                                                                              aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaagaa                                                        >  1:179421/1‑147 (MQ=255)
                                                                                               cGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAAt                                                                      <  6:53347/132‑1 (MQ=255)
                                                                                               cGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAAt                                                                      >  5:53347/1‑132 (MQ=255)
                                                                                               cGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAGTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaagaagcg                                                     >  6:295486/1‑147 (MQ=255)
                                                                                                 tGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTAAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCg                                                   <  3:225212/149‑1 (MQ=255)
                                                                                                  ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCgg                                                  >  1:252801/1‑149 (MQ=255)
                                                                                                    cGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAc                                                <  2:14414/149‑1 (MQ=255)
                                                                                                       gCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACaaa                                             <  7:18274/149‑1 (MQ=255)
                                                                                                        cAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAAc                                            <  8:241084/149‑1 (MQ=255)
                                                                                                           gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                         >  3:131294/1‑149 (MQ=255)
                                                                                                           gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                         <  2:179421/149‑1 (MQ=255)
                                                                                                            aCGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGAc                                        <  2:162418/149‑1 (MQ=255)
                                                                                                                tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCa                                    >  8:53020/1‑149 (MQ=255)
                                                                                                                   cTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATg                                  >  1:137123/1‑148 (MQ=255)
                                                                                                                      aaTATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgcag                               >  4:257124/1‑148 (MQ=255)
                                                                                                                          tCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATg                           <  1:28480/148‑1 (MQ=255)
                                                                                                                              gTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATggg                         <  7:325857/146‑1 (MQ=255)
                                                                                                                              gTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                          <  7:298990/145‑1 (MQ=255)
                                                                                                                                 cGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAg                    <  7:86657/148‑1 (MQ=255)
                                                                                                                                 cGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCCGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGc                   >  2:81509/1‑149 (MQ=255)
                                                                                                                                   tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGcc                  >  6:144053/5‑148 (MQ=255)
                                                                                                                                    gataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCa                >  7:28125/4‑149 (MQ=255)
                                                                                                                                      taaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATc              >  6:44819/2‑149 (MQ=255)
                                                                                                                                      cAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATc              <  5:31538/149‑1 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCg             >  7:129136/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGCGCGGACAAACGGACCGCATGCAGCATgg                          >  4:254814/1‑136 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAACCGGACCGCATGCAGCATGGGTAAAGCCCATCg             >  1:54932/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                          <  3:254814/136‑1 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCg             >  4:107612/1‑149 (MQ=255)
                                                                                                                                        aaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGc            >  7:311666/1‑149 (MQ=255)
                                                                                                                                              ccGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAgcgc      >  1:108493/1‑149 (MQ=255)
                                                                                                                                                  ttCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCg  >  8:310940/1‑149 (MQ=255)
                                                                                                                                                    cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCg  <  2:128171/147‑1 (MQ=255)
                                                                                                                                                    cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCg  <  2:137123/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCG  >  NC_000913/659056‑659350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑